Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   QPX86_RS18500 Genome accession   NZ_CP126972
Coordinates   4396383..4398095 (+) Length   570 a.a.
NCBI ID   WP_285163482.1    Uniprot ID   -
Organism   Shewanella goraebulensis strain DAU312     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4391383..4403095
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QPX86_RS18480 ampE 4392117..4392968 (-) 852 WP_285163478.1 beta-lactamase regulator AmpE -
  QPX86_RS18485 ampD 4393118..4393666 (-) 549 WP_285163479.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  QPX86_RS18490 nadC 4393915..4394793 (+) 879 WP_285163480.1 carboxylating nicotinate-nucleotide diphosphorylase -
  QPX86_RS18495 - 4395844..4396266 (+) 423 WP_285163481.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  QPX86_RS18500 pilB 4396383..4398095 (+) 1713 WP_285163482.1 type IV-A pilus assembly ATPase PilB Machinery gene
  QPX86_RS18505 pilC 4398171..4399439 (+) 1269 WP_285163483.1 type II secretion system F family protein Machinery gene
  QPX86_RS18510 pilD 4399487..4400404 (+) 918 WP_407696605.1 prepilin peptidase Machinery gene
  QPX86_RS18515 coaE 4400406..4401011 (+) 606 WP_285163485.1 dephospho-CoA kinase -
  QPX86_RS18520 zapD 4401021..4401758 (+) 738 WP_285163486.1 cell division protein ZapD -
  QPX86_RS18525 yacG 4401799..4402008 (+) 210 WP_285163487.1 DNA gyrase inhibitor YacG -
  QPX86_RS18530 mutT 4402023..4402418 (+) 396 WP_285163488.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63091.31 Da        Isoelectric Point: 5.2571

>NTDB_id=840906 QPX86_RS18500 WP_285163482.1 4396383..4398095(+) (pilB) [Shewanella goraebulensis strain DAU312]
MATSGLNLGLSTLFIRKNLLSEEQVSVAISQSRKSKKSLVTTIIQSKLLSSREIAELCYEEYGAPLLDLSEFDFSNIPEE
ILNKKLINNHKCLPLFKRGNRLFIATSDPTNIAALEDFQFSLGLHAEAIIVEDDKLNIALEKVLEEDISGLGLDGIDEEA
LAGIEVADTDKHHEEAESESSDDAPIVIYINKILTDAIRKGASDLHFEPYEKRYRIRFRIDGILHEVSEPPVNLSNRISA
RLKVMSKLDIAERRVPQDGRIKMKLSRTKSIDFRVSTLPTIWGEKIVMRILDSSSAQLGIEKLGYEDDQRLLYEEMLAKP
QGMILVTGPTGSGKTVSLYTGLNILNTEERNISTAEDPVEINLEGVNQVHINLKAGLTFASALRSFLRQDPDVVMVGEIR
DLETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLINMGVPGYNIASSVNLIIAQRLARRLCLECKTPEDVPVEELQRLGF
TQDIINTGFTVFKPVGCEHCSGGYKGRVGIYEVMRMTDEISRTIMEGGNSLEIARQAKTSGMRDLRLSGLLKVTHGITSI
AEVNRVTSFG

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=840906 QPX86_RS18500 WP_285163482.1 4396383..4398095(+) (pilB) [Shewanella goraebulensis strain DAU312]
ATGGCAACGTCTGGTCTTAATTTAGGTTTATCAACATTATTTATCCGCAAAAATTTGTTATCTGAAGAGCAAGTTTCTGT
TGCCATTAGTCAATCAAGAAAATCAAAAAAATCACTTGTCACCACCATAATTCAATCTAAACTACTTTCTTCGCGCGAGA
TAGCAGAGCTCTGCTATGAAGAGTATGGTGCTCCGCTTTTAGATTTATCAGAGTTTGACTTTAGTAATATTCCTGAAGAA
ATTCTTAATAAAAAGCTGATTAATAATCATAAATGTCTGCCTCTATTTAAACGAGGCAATCGACTTTTTATAGCAACTTC
TGACCCAACTAATATCGCAGCTTTAGAAGACTTCCAATTCAGCCTAGGGCTACATGCTGAAGCCATTATTGTGGAAGATG
ACAAGTTAAATATAGCGCTAGAGAAGGTACTTGAAGAAGACATTTCAGGCCTTGGTCTAGATGGTATTGACGAAGAGGCC
CTGGCTGGCATTGAAGTAGCAGATACCGACAAACACCATGAAGAAGCCGAATCAGAAAGCAGTGACGATGCCCCTATTGT
TATTTACATTAATAAAATATTAACTGATGCCATCCGTAAAGGCGCCTCAGATTTACATTTTGAACCTTACGAAAAACGCT
ACCGGATACGTTTTCGAATCGATGGTATTTTGCACGAAGTGTCCGAGCCTCCGGTCAACTTATCAAACCGTATTTCAGCT
CGATTAAAGGTGATGTCAAAGTTGGACATTGCCGAGCGCCGGGTACCACAAGATGGCCGAATTAAAATGAAGCTGTCACG
CACAAAATCAATTGATTTTCGTGTCAGTACCTTGCCCACTATTTGGGGCGAAAAGATTGTAATGCGTATTTTGGATTCAT
CTTCTGCGCAATTAGGCATTGAAAAACTCGGTTATGAAGACGACCAGCGTTTGCTTTATGAAGAAATGCTAGCAAAACCC
CAAGGGATGATTCTAGTTACAGGTCCTACTGGTTCAGGTAAGACTGTCTCGTTATACACAGGCCTTAATATTCTCAATAC
TGAAGAACGTAATATTTCCACTGCCGAAGATCCAGTTGAGATTAATCTTGAAGGGGTTAACCAAGTTCACATTAATTTAA
AGGCAGGCTTAACCTTCGCATCTGCCCTGAGATCATTTTTACGTCAAGATCCTGATGTAGTCATGGTCGGTGAAATTCGA
GATTTAGAAACCGCAGAAATTGCCATTAAAGCAGCCCAAACCGGTCACTTGGTATTATCAACACTGCATACTAACTCAGC
AGCCGAAACTCTAACCCGTTTAATCAACATGGGCGTACCAGGTTATAACATCGCAAGCTCTGTGAATTTAATTATTGCTC
AGCGTCTTGCCCGCCGCTTATGCCTTGAATGCAAAACCCCTGAAGATGTCCCTGTAGAAGAACTACAACGACTTGGCTTT
ACTCAAGATATCATTAATACTGGGTTCACTGTTTTTAAGCCTGTTGGCTGCGAGCATTGTTCTGGTGGTTATAAAGGGCG
GGTCGGTATCTATGAAGTGATGAGAATGACCGATGAAATCTCCAGAACCATTATGGAAGGTGGCAATTCGCTTGAAATAG
CGAGACAGGCTAAAACCAGTGGCATGAGAGATTTAAGATTATCAGGTTTACTCAAAGTAACCCATGGAATAACCAGTATT
GCCGAAGTTAATCGAGTAACTAGCTTTGGTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-143)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

59.147

98.772

0.584
  pilB Acinetobacter baylyi ADP1

58.289

98.421

0.574
  pilB Legionella pneumophila strain ERS1305867

56.306

98.772

0.556
  pilB Vibrio parahaemolyticus RIMD 2210633

53.191

98.947

0.526
  pilB Vibrio cholerae strain A1552

52.753

98.772

0.521
  pilB Vibrio campbellii strain DS40M4

51.418

98.947

0.509
  pilF Neisseria gonorrhoeae MS11

46.82

99.298

0.465
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.318

97.719

0.384
  pilF Thermus thermophilus HB27

38.351

97.895

0.375