Detailed information NTG0839361

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: LLE01_RS05680
• Genome accession: NZ_CP126675
• Coordinates: 887728..888375 (+)
• Length: 215 a.a.
• NCBI ID: WP_010906131.1
• Uniprot ID: A0A3N6KQH1
• Organism: Lactococcus lactis subsp. lactis strain E.0.1
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 882728..893375

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LLE01_RS05655 (LLE01_05640)	-	882857..884035 (+)	1179	WP_021214596.1	SLC13 family permease	-
LLE01_RS05660 (LLE01_05645)	-	884208..884771 (+)	564	WP_004255233.1	GNAT family N-acetyltransferase	-
LLE01_RS05665 (LLE01_05650)	-	884875..885273 (+)	399	WP_004255236.1	hypothetical protein	-
LLE01_RS05670 (LLE01_05655)	-	885378..886310 (+)	933	WP_004255238.1	ABC transporter ATP-binding protein	-
LLE01_RS05675 (LLE01_05660)	-	886307..887668 (+)	1362	WP_021214597.1	ABC transporter permease	-
LLE01_RS05680 (LLE01_05665)	comEA	887728..888375 (+)	648	WP_010906131.1	ComEA family DNA-binding protein	Machinery gene
LLE01_RS05685 (LLE01_05670)	comEC	888356..890566 (+)	2211	WP_010906130.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
LLE01_RS05690 (LLE01_05675)	-	890851..891627 (+)	777	WP_004255244.1	alpha/beta hydrolase family protein	-
LLE01_RS05695 (LLE01_05680)	-	891811..892026 (+)	216	WP_004255250.1	F0F1 ATP synthase subunit C	-
LLE01_RS05700 (LLE01_05685)	atpB	892071..892784 (+)	714	WP_004255255.1	F0F1 ATP synthase subunit A	-
LLE01_RS05705 (LLE01_05690)	atpF	892799..893305 (+)	507	WP_010906128.1	F0F1 ATP synthase subunit B	-

Sequence

Protein

• Length: 215 a.a.
• Molecular weight: 23624.76 Da
• Isoelectric Point: 4.8659

>NTDB_id=839361 LLE01_RS05680 WP_010906131.1 887728..888375(+) (comEA) [Lactococcus lactis subsp. lactis strain E.0.1]
MDKILEKVKEYWKMIVLVVCGLIAGGIFYVLTNGQKPTTNLSVENLSSVSRQSSVSKFSEPNEKSVSKIMVDLKGAVTKP
NVYQISSDERLVDLIKEAGGFTDQADQKSINLSAKLKDEEVIYVPKIGENSSTENTDSSANSVTSQVSTTTEKININQAD
LTELQKLTGVGQKKAQDIIDFRTKNGDFKSLEDLGKVSGFGDKTLEKLKDELCFE

Nucleotide

• Length: 648 bp

>NTDB_id=839361 LLE01_RS05680 WP_010906131.1 887728..888375(+) (comEA) [Lactococcus lactis subsp. lactis strain E.0.1]
ATGGATAAGATTTTAGAAAAAGTAAAAGAATATTGGAAAATGATTGTTTTAGTTGTTTGTGGGCTCATTGCTGGTGGGAT
TTTTTACGTTTTAACCAACGGTCAAAAGCCAACTACAAATCTGTCAGTAGAAAATTTAAGTTCTGTCAGCAGGCAAAGCT
CTGTCAGTAAATTTAGTGAACCTAATGAAAAATCTGTCAGTAAAATTATGGTTGATTTAAAAGGTGCGGTAACAAAGCCT
AATGTCTATCAAATTTCGTCAGATGAACGTCTAGTTGATTTAATTAAAGAAGCTGGTGGTTTTACTGACCAAGCTGACCA
GAAATCAATTAATCTGTCAGCAAAATTAAAAGATGAAGAAGTAATTTATGTCCCAAAAATTGGTGAAAATTCAAGCACAG
AAAATACTGACAGCTCTGCTAATTCGGTCACTAGTCAAGTCTCAACAACCACTGAAAAAATAAATATCAATCAAGCAGAT
TTGACTGAACTACAAAAATTAACAGGTGTTGGTCAGAAAAAAGCTCAAGACATCATCGATTTTCGGACGAAAAATGGTGA
TTTCAAATCACTTGAAGACTTGGGAAAAGTTTCTGGTTTTGGAGATAAAACACTAGAAAAATTGAAAGATGAGTTGTGTT
TTGAATAA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (70-125)

• Helix-hairpin-helix motif (HHH_3) (151-212)

3D structure

• AlphaFold DB: A0A3N6KQH1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Lactococcus lactis subsp. cremoris KW2	76.147	100	0.772
comEA/celA/cilE	Streptococcus mitis SK321	44.907	100	0.451
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	43.578	100	0.442
comEA/celA/cilE	Streptococcus pneumoniae Rx1	41.553	100	0.423
comEA/celA/cilE	Streptococcus pneumoniae D39	41.553	100	0.423
comEA/celA/cilE	Streptococcus pneumoniae R6	41.553	100	0.423
comEA/celA/cilE	Streptococcus mitis NCTC 12261	41.743	100	0.423
comEA	Latilactobacillus sakei subsp. sakei 23K	36.564	100	0.386