Detailed information NTG0838036

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: LL303_RS09690
• Genome accession: NZ_CP126467
• Coordinates: 1909966..1910613 (-)
• Length: 215 a.a.
• NCBI ID: WP_010906131.1
• Uniprot ID: A0A3N6KQH1
• Organism: Lactococcus lactis subsp. lactis strain 303
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1904966..1915613

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LL303_RS09665 (LL303_09640)	atpF	1905035..1905541 (-)	507	WP_023164471.1	F0F1 ATP synthase subunit B	-
LL303_RS09670 (LL303_09645)	atpB	1905556..1906269 (-)	714	WP_004255255.1	F0F1 ATP synthase subunit A	-
LL303_RS09675 (LL303_09650)	-	1906314..1906529 (-)	216	WP_004255250.1	F0F1 ATP synthase subunit C	-
LL303_RS09680 (LL303_09655)	-	1906714..1907490 (-)	777	WP_004255244.1	alpha/beta hydrolase family protein	-
LL303_RS09685 (LL303_09660)	comEC	1907775..1909985 (-)	2211	WP_023164472.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
LL303_RS09690 (LL303_09665)	comEA	1909966..1910613 (-)	648	WP_010906131.1	ComEA family DNA-binding protein	Machinery gene
LL303_RS09695 (LL303_09670)	-	1910673..1912034 (-)	1362	WP_023164473.1	ABC transporter permease	-
LL303_RS09700 (LL303_09675)	-	1912031..1912963 (-)	933	WP_004255238.1	ABC transporter ATP-binding protein	-
LL303_RS09705 (LL303_09680)	-	1913068..1913466 (-)	399	WP_004255236.1	hypothetical protein	-
LL303_RS09710 (LL303_09685)	-	1913570..1914133 (-)	564	WP_004255233.1	GNAT family N-acetyltransferase	-
LL303_RS09715 (LL303_09690)	-	1914306..1915484 (-)	1179	WP_012898345.1	SLC13 family permease	-

Sequence

Protein

• Length: 215 a.a.
• Molecular weight: 23624.76 Da
• Isoelectric Point: 4.8659

>NTDB_id=838036 LL303_RS09690 WP_010906131.1 1909966..1910613(-) (comEA) [Lactococcus lactis subsp. lactis strain 303]
MDKILEKVKEYWKMIVLVVCGLIAGGIFYVLTNGQKPTTNLSVENLSSVSRQSSVSKFSEPNEKSVSKIMVDLKGAVTKP
NVYQISSDERLVDLIKEAGGFTDQADQKSINLSAKLKDEEVIYVPKIGENSSTENTDSSANSVTSQVSTTTEKININQAD
LTELQKLTGVGQKKAQDIIDFRTKNGDFKSLEDLGKVSGFGDKTLEKLKDELCFE

Nucleotide

• Length: 648 bp

>NTDB_id=838036 LL303_RS09690 WP_010906131.1 1909966..1910613(-) (comEA) [Lactococcus lactis subsp. lactis strain 303]
ATGGATAAGATTTTAGAAAAAGTAAAAGAATATTGGAAAATGATTGTTTTAGTTGTTTGTGGGCTCATTGCTGGTGGGAT
TTTTTACGTTTTAACCAACGGTCAAAAGCCAACTACAAATCTGTCAGTAGAAAATTTAAGTTCTGTCAGCAGGCAAAGCT
CTGTCAGTAAATTTAGTGAACCTAATGAAAAATCTGTCAGTAAAATTATGGTTGATTTAAAAGGTGCGGTAACAAAGCCT
AATGTCTATCAAATTTCGTCAGATGAACGTCTAGTTGATTTAATTAAAGAAGCTGGTGGTTTTACTGACCAAGCTGACCA
GAAATCAATTAATCTGTCAGCAAAATTAAAAGATGAAGAAGTAATTTATGTCCCAAAAATTGGTGAAAATTCAAGCACAG
AAAATACTGACAGCTCTGCTAATTCGGTCACTAGTCAAGTCTCAACAACCACTGAAAAAATAAATATCAATCAAGCAGAT
TTGACTGAACTACAAAAATTAACAGGTGTTGGTCAGAAAAAAGCTCAAGACATCATCGATTTTCGGACGAAAAATGGTGA
TTTCAAATCACTTGAAGACTTGGGAAAAGTTTCTGGTTTTGGAGATAAAACACTAGAAAAATTGAAAGATGAGTTGTGTT
TTGAATAA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (70-125)

• Helix-hairpin-helix motif (HHH_3) (151-212)

3D structure

• AlphaFold DB: A0A3N6KQH1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Lactococcus lactis subsp. cremoris KW2	76.147	100	0.772
comEA/celA/cilE	Streptococcus mitis SK321	44.907	100	0.451
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	43.578	100	0.442
comEA/celA/cilE	Streptococcus pneumoniae Rx1	41.553	100	0.423
comEA/celA/cilE	Streptococcus pneumoniae D39	41.553	100	0.423
comEA/celA/cilE	Streptococcus pneumoniae R6	41.553	100	0.423
comEA/celA/cilE	Streptococcus mitis NCTC 12261	41.743	100	0.423
comEA	Latilactobacillus sakei subsp. sakei 23K	36.564	100	0.386