Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   VA249_RS12950 Genome accession   NZ_AP024165
Coordinates   2810911..2812590 (-) Length   559 a.a.
NCBI ID   WP_420864276.1    Uniprot ID   -
Organism   Vibrio alfacsensis strain 04Ya108     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2805911..2817590
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VA249_RS12930 (VYA_24280) - 2806600..2807439 (-) 840 WP_221068214.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  VA249_RS24335 - 2807509..2807898 (-) 390 Protein_2418 SPOR domain-containing protein -
  VA249_RS12935 (VYA_24290) - 2807980..2809023 (-) 1044 Protein_2419 ATP-binding protein -
  VA249_RS12940 (VYA_24300) aroB 2809075..2810175 (-) 1101 WP_221068216.1 3-dehydroquinate synthase -
  VA249_RS12945 (VYA_24310) aroK 2810204..2810722 (-) 519 WP_099080818.1 shikimate kinase AroK -
  VA249_RS12950 (VYA_24320) pilQ 2810911..2812590 (-) 1680 WP_420864276.1 type IV pilus secretin PilQ Machinery gene
  VA249_RS12955 (VYA_24330) pilP 2812650..2813159 (-) 510 WP_221068217.1 pilus assembly protein PilP Machinery gene
  VA249_RS12960 (VYA_24340) pilO 2813149..2813742 (-) 594 WP_221068218.1 type 4a pilus biogenesis protein PilO Machinery gene
  VA249_RS12965 (VYA_24350) pilN 2813735..2814310 (-) 576 WP_221068219.1 PilN domain-containing protein Machinery gene
  VA249_RS12970 (VYA_24360) pilM 2814294..2815307 (-) 1014 WP_221068220.1 type IV pilus biogenesis protein PilM Machinery gene

Sequence

Protein


Download         Length: 559 a.a.        Molecular weight: 61538.20 Da        Isoelectric Point: 4.6114

>NTDB_id=83741 VA249_RS12950 WP_420864276.1 2810911..2812590(-) (pilQ) [Vibrio alfacsensis strain 04Ya108]
MVVGFFSVWLFMFFASEGRAKASPPNELKSIDFRADNNTEAVIMIELTSSSVVVDIQRERDGLSIELLNTSVSDNQLHLL
DVRDFSTVIKQIDVSRYHSATRLLAYTTDEYRYDYRLSGRWLEVTVVKEKSTKQAQSQNILKNKGRLISINFQDIPVRNV
LQLIADYNDFNLVVSDSVSGNLTLRLDDVPWPQVLDIILQVKGLDKRVDGNVILIAPLDELELREKQRLEKQKLEQEIGE
LSSEIIKVNFAKASDVAEMINGEGNISMLSERGSMTVDERTNSLLVRELPENIEVIREIIEALDIPVKQVQIEARIVTIN
EGNLDELGVRWGFTSINGSSTVGGSIENNLATLGLYDGGVGIDDFLNVNLAATNPSATSLAFQVAKLGAGTLLDLELSAL
QQESKAEIISSPRLITTNKKPAYIEQGTEIPYLESSSSGATSVTFKKAVLSLKVTPQITPDNRLVLDLSVTQDRPGQVVK
TGTGEAVAIDTQRIGTQVLVNNGETVVLGGIFQHSLSNAVDKVPLLGDLPLLGALFRRSYEKVGKSELLIFVTPKVVIQ

Nucleotide


Download         Length: 1680 bp        

>NTDB_id=83741 VA249_RS12950 WP_420864276.1 2810911..2812590(-) (pilQ) [Vibrio alfacsensis strain 04Ya108]
ATGGTGGTTGGTTTTTTCTCTGTGTGGCTATTTATGTTTTTTGCATCAGAGGGGAGAGCAAAGGCGTCACCACCGAATGA
ATTAAAATCGATAGACTTCCGTGCAGATAACAATACAGAAGCGGTGATCATGATTGAGCTAACGTCATCGTCTGTCGTGG
TTGATATACAACGTGAAAGAGATGGGTTAAGTATTGAGCTGCTTAATACTTCGGTCAGTGATAACCAACTGCACCTTTTG
GACGTGAGAGATTTCTCTACCGTCATTAAGCAAATTGACGTTTCTCGTTATCATTCGGCCACGCGTTTGTTGGCCTATAC
GACCGATGAATACCGCTATGACTATCGCCTGTCAGGACGCTGGTTAGAAGTGACAGTGGTGAAAGAAAAGTCAACTAAGC
AAGCACAAAGCCAGAACATTTTGAAGAATAAAGGGCGTTTAATCTCTATCAATTTTCAAGATATTCCGGTGCGAAATGTA
TTGCAGTTAATTGCTGACTATAACGATTTCAATCTCGTGGTATCGGATTCTGTGTCGGGTAATCTGACGTTACGCTTAGA
TGACGTACCTTGGCCACAAGTGTTAGATATCATTTTGCAAGTGAAAGGATTGGATAAACGGGTTGATGGCAATGTTATTT
TGATTGCCCCATTAGATGAACTGGAGCTAAGAGAAAAGCAGCGTCTTGAAAAGCAAAAGCTTGAGCAAGAAATAGGAGAG
CTTTCCTCTGAGATCATCAAAGTGAATTTTGCAAAAGCCTCGGACGTTGCGGAAATGATCAATGGTGAGGGTAATATCAG
CATGCTTTCTGAGCGAGGAAGTATGACCGTTGATGAGCGCACGAATTCATTGTTGGTGCGAGAATTACCAGAGAACATTG
AAGTCATTCGCGAAATTATAGAAGCGTTAGACATTCCCGTGAAACAAGTACAGATTGAAGCTCGCATTGTCACCATTAAT
GAGGGGAACTTGGATGAGCTAGGGGTTCGTTGGGGTTTTACAAGTATAAATGGCAGTTCAACGGTTGGAGGCTCGATTGA
AAATAATCTCGCCACACTCGGTTTATATGATGGTGGTGTTGGTATTGATGATTTTCTTAACGTTAACCTGGCGGCGACCA
ACCCAAGTGCCACTAGTCTTGCTTTTCAAGTGGCAAAACTAGGGGCAGGGACTTTGCTTGATTTAGAGTTGTCTGCCTTG
CAGCAGGAGTCAAAAGCAGAAATTATCTCGAGTCCACGGTTGATCACCACCAATAAGAAACCCGCTTATATTGAGCAGGG
AACGGAAATCCCGTATTTAGAATCGTCATCAAGTGGGGCGACGTCGGTGACATTTAAAAAAGCAGTATTGAGTTTAAAAG
TTACGCCTCAGATAACGCCAGATAATCGCTTAGTGTTGGATTTGAGTGTGACTCAAGACCGACCTGGTCAAGTGGTGAAA
ACAGGCACGGGAGAAGCGGTTGCAATTGATACTCAGAGAATTGGCACTCAAGTTTTGGTCAATAATGGTGAAACGGTGGT
ATTGGGTGGTATTTTCCAACATAGCCTCAGTAATGCCGTTGACAAAGTCCCTTTACTTGGTGATTTACCCTTGCTTGGCG
CGCTTTTTCGCCGAAGTTATGAAAAAGTAGGCAAAAGTGAGTTGTTGATTTTCGTGACGCCAAAGGTCGTAATTCAGTAG

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(170-217)

Bacterial type II/III secretion system short domain (Secretin_N)

(244-309)

Bacterial type II and III secretion system protein (Secretin)

(399-557)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

82.689

97.138

0.803
  pilQ Vibrio cholerae strain A1552

70.841

100

0.708
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

70.841

100

0.708
  pilQ Pseudomonas aeruginosa PAK

39.86

100

0.408
  pilQ Legionella pneumophila strain ERS1305867

40.959

96.959

0.397
  pilQ Legionella pneumophila strain Lp02

40.959

96.959

0.397
  comQ Acinetobacter baylyi ADP1

38.624

100

0.392
  pilQ Acinetobacter baumannii D1279779

35.911

100

0.374
  pilQ Acinetobacter baumannii strain A118

35.911

100

0.374

Multiple sequence alignment