Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   JN439_RS02815 Genome accession   NZ_AP024136
Coordinates   570991..572100 (-) Length   369 a.a.
NCBI ID   WP_053288105.1    Uniprot ID   -
Organism   Aeromonas caviae strain KAM329     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 565991..577100
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JN439_RS02790 (KAM329_005320) - 566123..566350 (+) 228 WP_049636474.1 hypothetical protein -
  JN439_RS02795 (KAM329_005330) brnQ 566441..567703 (-) 1263 WP_049636536.1 branched-chain amino acid transport system II carrier protein -
  JN439_RS02800 (KAM329_005340) - 567939..568649 (-) 711 WP_049636473.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  JN439_RS02805 (KAM329_005350) srmB 568777..569997 (+) 1221 WP_041210934.1 ATP-dependent RNA helicase SrmB -
  JN439_RS02810 (KAM329_005360) yaaA 570185..570958 (-) 774 WP_053288104.1 peroxide stress protein YaaA -
  JN439_RS02815 (KAM329_005370) pilU 570991..572100 (-) 1110 WP_053288105.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  JN439_RS02820 (KAM329_005380) pilT 572124..573158 (-) 1035 WP_010673050.1 type IVa pilus ATPase TapT Machinery gene
  JN439_RS02825 (KAM329_005390) - 573198..573899 (+) 702 WP_042014924.1 YggS family pyridoxal phosphate-dependent enzyme -
  JN439_RS02830 (KAM329_005400) proC 574023..574847 (+) 825 WP_053288106.1 pyrroline-5-carboxylate reductase -
  JN439_RS02835 (KAM329_005410) - 574871..575422 (+) 552 WP_053288107.1 YggT family protein -
  JN439_RS02840 (KAM329_005420) yggU 575422..575721 (+) 300 WP_010673054.1 DUF167 family protein YggU -
  JN439_RS02845 (KAM329_005430) - 575740..576159 (+) 420 WP_053288108.1 DUF4426 domain-containing protein -
  JN439_RS02850 (KAM329_005440) - 576235..577065 (-) 831 WP_041214463.1 CPBP family intramembrane glutamic endopeptidase -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 40773.91 Da        Isoelectric Point: 6.1839

>NTDB_id=83631 JN439_RS02815 WP_053288105.1 570991..572100(-) (pilU) [Aeromonas caviae strain KAM329]
MNMDVLLAALVEQKGSDLFITVDAPPTLKVNGRLVSLGPAPLDKTTALALIRESLDETHFERFLHTREANYAIQRGSLGR
FRVSAFWQQDMPGMVLRRIETRIPTFDELVLPPILQEVAMAKRGLVLFVGATGAGKSTTQAAMIGFRNQHGDGHILTVED
PVEFVHQHDRCLVTQREVGIDTASFDVALKNSLRQAPDVILIGEIRSQETMEFAIQFAETGHLCLATLHANNANQALDRI
LHLVPQEKHCQFLFDLSFNLRAIVAQQLLPSAHGQRRVAAFEILLNTPLVTDIIRKGEMHRLKEVMTKSGELGMQTFDQA
LFGLFCAGQIGYSEALAHADSANDLRLLIKLSGREQLGAGTLDNVALDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=83631 JN439_RS02815 WP_053288105.1 570991..572100(-) (pilU) [Aeromonas caviae strain KAM329]
ATGAACATGGATGTTCTGCTCGCCGCACTGGTTGAACAAAAGGGCTCGGATCTCTTCATCACGGTCGATGCCCCCCCGAC
CCTCAAGGTCAACGGGCGTCTGGTGTCGCTCGGGCCGGCTCCCCTCGACAAGACGACGGCGCTGGCGTTGATCAGAGAAA
GCCTGGACGAGACCCACTTCGAGCGCTTCCTGCACACCCGCGAGGCCAACTACGCCATCCAGCGAGGCTCGCTGGGGCGC
TTTCGGGTCAGCGCCTTCTGGCAACAGGATATGCCGGGCATGGTGCTGCGCCGCATCGAGACCCGCATCCCCACCTTTGA
CGAGCTGGTGCTGCCTCCCATCCTGCAGGAGGTCGCCATGGCCAAACGCGGGCTGGTGCTCTTCGTCGGCGCCACTGGCG
CCGGCAAGTCCACCACCCAGGCGGCCATGATCGGCTTTCGCAACCAGCACGGTGACGGCCACATCCTGACGGTGGAGGAT
CCGGTGGAGTTCGTCCATCAGCACGATCGCTGTCTGGTGACCCAGCGGGAGGTGGGGATCGACACCGCATCCTTCGATGT
CGCCCTCAAAAACTCCCTACGCCAGGCCCCGGATGTCATCCTCATCGGTGAAATCCGCTCCCAGGAGACCATGGAGTTCG
CCATCCAGTTCGCCGAGACGGGGCACCTCTGCCTCGCCACCTTGCACGCCAACAATGCCAACCAGGCGCTGGATCGCATC
CTGCATCTGGTCCCCCAGGAGAAGCACTGCCAGTTCCTGTTCGATCTCTCCTTCAACCTGCGCGCCATCGTCGCCCAGCA
GCTGCTCCCCTCCGCTCACGGGCAGCGCCGGGTCGCCGCCTTCGAGATCCTGCTCAACACCCCGCTCGTCACCGACATCA
TCCGCAAGGGAGAGATGCACAGACTCAAGGAGGTGATGACCAAATCCGGCGAGCTCGGCATGCAGACCTTCGATCAGGCG
CTGTTCGGCCTGTTCTGCGCCGGCCAAATTGGCTACAGTGAGGCCCTTGCCCATGCCGACTCCGCCAACGACCTGCGACT
GCTCATCAAGCTGTCGGGTCGCGAGCAGCTGGGCGCAGGCACTCTCGACAACGTGGCCCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.598

99.729

0.604
  pilU Pseudomonas stutzeri DSM 10701

58.974

95.122

0.561
  pilU Acinetobacter baylyi ADP1

57.79

95.664

0.553
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.155

91.057

0.393
  pilT Legionella pneumophila strain Lp02

40.816

92.954

0.379
  pilT Legionella pneumophila strain ERS1305867

40.816

92.954

0.379

Multiple sequence alignment