Detailed information NTG0836111

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: QNH19_RS12450
• Genome accession: NZ_CP126104
• Coordinates: 2563702..2564316 (-)
• Length: 204 a.a.
• NCBI ID: WP_043021637.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain MB7_B11
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2558702..2569316

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QNH19_RS12420 (QNH19_12420)	rpsT	2559005..2559271 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
QNH19_RS12425 (QNH19_12425)	holA	2559288..2560329 (-)	1042	Protein_2405	DNA polymerase III subunit delta	-
QNH19_RS12430 (QNH19_12430)	-	2560369..2560520 (-)	152	Protein_2406	hypothetical protein	-
QNH19_RS12435 (QNH19_12435)	-	2560561..2560695 (+)	135	WP_003152870.1	YqzM family protein	-
QNH19_RS12440 (QNH19_12440)	comEC	2560714..2563065 (-)	2352	WP_283886900.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
QNH19_RS12445 (QNH19_12445)	-	2563066..2563635 (-)	570	WP_003152868.1	ComE operon protein 2	-
QNH19_RS12450 (QNH19_12450)	comEA	2563702..2564316 (-)	615	WP_043021637.1	helix-hairpin-helix domain-containing protein	Machinery gene
QNH19_RS12455 (QNH19_12455)	comER	2564375..2565196 (+)	822	WP_012118027.1	late competence protein ComER	-
QNH19_RS12460 (QNH19_12460)	-	2565265..2566002 (-)	738	WP_076424998.1	class I SAM-dependent methyltransferase	-
QNH19_RS12465 (QNH19_12465)	rsfS	2565999..2566355 (-)	357	WP_007408260.1	ribosome silencing factor	-
QNH19_RS12470 (QNH19_12470)	yqeK	2566373..2566933 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
QNH19_RS12475 (QNH19_12475)	-	2566923..2567492 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
QNH19_RS12480 (QNH19_12480)	yhbY	2567503..2567793 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
QNH19_RS12485 (QNH19_12485)	aroE	2567787..2568629 (-)	843	WP_283886903.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22165.31 Da
• Isoelectric Point: 6.4888

>NTDB_id=836111 QNH19_RS12450 WP_043021637.1 2563702..2564316(-) (comEA) [Bacillus velezensis strain MB7_B11]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTAADKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=836111 QNH19_RS12450 WP_043021637.1 2563702..2564316(-) (comEA) [Bacillus velezensis strain MB7_B11]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGCATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCTGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGCCGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGGGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CGGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA	Latilactobacillus sakei subsp. sakei 23K	32.759	100	0.373
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae D39	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae R6	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363