Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ZMTM_RS10475 Genome accession   NZ_AP024110
Coordinates   2180404..2181540 (-) Length   378 a.a.
NCBI ID   WP_221763798.1    Uniprot ID   A0A8D5JZJ8
Organism   Methyloradius palustris strain Zm11     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2175404..2186540
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ZMTM_RS10445 (ZMTM_19900) ribBA 2175410..2176495 (+) 1086 WP_221763793.1 bifunctional 3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP cyclohydrolase II -
  ZMTM_RS10450 (ZMTM_19910) - 2176559..2177119 (+) 561 WP_221763794.1 YqgE/AlgH family protein -
  ZMTM_RS10455 (ZMTM_19920) ruvX 2177158..2177676 (+) 519 WP_221763795.1 Holliday junction resolvase RuvX -
  ZMTM_RS10460 (ZMTM_19930) pyrR 2177673..2178176 (+) 504 WP_404804665.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  ZMTM_RS10465 (ZMTM_19940) - 2178176..2179126 (+) 951 WP_221763796.1 aspartate carbamoyltransferase catalytic subunit -
  ZMTM_RS10470 (ZMTM_19950) - 2179126..2180400 (+) 1275 WP_221763797.1 dihydroorotase -
  ZMTM_RS10475 (ZMTM_19960) pilU 2180404..2181540 (-) 1137 WP_221763798.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ZMTM_RS10480 (ZMTM_19970) pilT 2181585..2182628 (-) 1044 WP_221763799.1 type IV pilus twitching motility protein PilT Machinery gene
  ZMTM_RS10485 (ZMTM_19980) - 2182681..2183373 (+) 693 WP_221763800.1 YggS family pyridoxal phosphate-dependent enzyme -
  ZMTM_RS10490 (ZMTM_19990) proC 2183435..2184244 (+) 810 WP_221763801.1 pyrroline-5-carboxylate reductase -
  ZMTM_RS10495 (ZMTM_20000) - 2184250..2184825 (+) 576 WP_221763802.1 YggT family protein -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42086.22 Da        Isoelectric Point: 6.1155

>NTDB_id=83494 ZMTM_RS10475 WP_221763798.1 2180404..2181540(-) (pilU) [Methyloradius palustris strain Zm11]
MDQGQAEKFVFDLLRMMIAKKASDLFITAGFPPAMKIDGKMTPVTNQALSAQHAREIARSIMNDKQASEFDATRECNFAI
SIPNVARFRVNAFVQRGSTGLVFRTITSRIPNFEELNLPSVLKDIALTKRGLVIFVGGTGSGKSTSLAAMIGYRNEHSYG
HIITIEDPIEYIHDHKNCIITQREVGVDTENWFAALKNTLRQAPDVILIGEIRDRETMDYAIAFAETGHLCMATLHANST
NQALDRIINFFPEERRAQLLMDLSLNVRAFVSQRLIPKVDGKGRSAAVEVMLNSPLISDLIFKGDVHEIKEIMSKSRELG
MQTFDQSLFDLYEAGDISYEDALRNADSVNDVRLKIKLEGRAAQDKNMAEGLDGLGIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=83494 ZMTM_RS10475 WP_221763798.1 2180404..2181540(-) (pilU) [Methyloradius palustris strain Zm11]
ATGGATCAAGGCCAGGCAGAAAAATTCGTTTTTGATTTATTGCGCATGATGATTGCAAAAAAAGCATCTGACCTTTTCAT
TACGGCAGGATTTCCGCCCGCAATGAAGATCGATGGCAAGATGACACCAGTCACCAATCAGGCCTTGAGTGCACAGCATG
CCCGCGAAATCGCGCGTTCCATCATGAATGACAAACAGGCTTCAGAGTTTGATGCGACGCGTGAATGTAACTTTGCCATC
AGCATTCCCAATGTGGCGCGTTTTCGCGTCAATGCCTTTGTGCAGCGAGGTTCTACTGGTCTGGTATTCCGTACTATTAC
ATCAAGAATCCCAAATTTTGAGGAGCTCAATCTACCATCTGTACTTAAAGACATTGCACTAACCAAGCGCGGTTTAGTGA
TATTTGTGGGCGGTACGGGTTCAGGTAAGTCAACCTCACTGGCCGCCATGATTGGTTATCGGAACGAACACAGCTACGGC
CATATCATTACGATTGAAGACCCGATTGAATATATTCATGACCATAAGAACTGCATCATTACGCAACGTGAAGTCGGTGT
GGATACGGAAAACTGGTTTGCAGCATTGAAAAACACATTACGCCAGGCACCCGATGTGATCCTGATTGGTGAAATTCGTG
ATCGCGAGACCATGGATTATGCGATTGCCTTTGCTGAAACAGGTCACCTGTGTATGGCGACTTTACATGCAAACAGTACT
AACCAAGCATTAGATCGTATTATCAACTTCTTCCCTGAAGAGCGCCGTGCACAATTATTAATGGATTTATCCCTCAACGT
GCGTGCCTTTGTTTCTCAACGCTTGATTCCTAAAGTGGACGGCAAAGGCCGCTCTGCTGCAGTTGAGGTGATGTTGAACT
CGCCATTGATTTCAGACTTGATCTTCAAGGGCGATGTACATGAAATCAAGGAAATCATGTCCAAGTCACGCGAGCTTGGC
ATGCAAACATTTGACCAATCACTGTTTGATCTATACGAAGCAGGCGATATTAGTTATGAAGACGCCTTGCGTAACGCCGA
TTCAGTGAACGATGTGCGCCTCAAGATCAAGCTTGAAGGCCGCGCCGCGCAGGATAAGAATATGGCTGAAGGGCTTGATG
GGCTGGGCATTGTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(84-256)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

64.451

91.534

0.59
  pilU Acinetobacter baylyi ADP1

60.227

93.122

0.561
  pilU Vibrio cholerae strain A1552

56.598

90.212

0.511
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.51

89.153

0.397
  pilT Acinetobacter nosocomialis M2

43.844

88.095

0.386
  pilT Acinetobacter baumannii D1279779

43.844

88.095

0.386
  pilT Acinetobacter baumannii strain A118

43.844

88.095

0.386
  pilT Pseudomonas aeruginosa PAK

43.323

89.153

0.386
  pilT Acinetobacter baylyi ADP1

43.544

88.095

0.384
  pilT Legionella pneumophila strain Lp02

43.243

88.095

0.381
  pilT Legionella pneumophila strain ERS1305867

43.243

88.095

0.381
  pilT Neisseria meningitidis 8013

42.059

89.947

0.378
  pilT Pseudomonas stutzeri DSM 10701

42.433

89.153

0.378
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.47

87.831

0.373
  pilT Vibrio cholerae strain A1552

42.47

87.831

0.373
  pilT Neisseria gonorrhoeae MS11

41.593

89.683

0.373

Multiple sequence alignment