Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ZMTM_RS10480 Genome accession   NZ_AP024110
Coordinates   2181585..2182628 (-) Length   347 a.a.
NCBI ID   WP_221763799.1    Uniprot ID   A0A8D5K1G4
Organism   Methyloradius palustris strain Zm11     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2176585..2187628
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ZMTM_RS10455 (ZMTM_19920) ruvX 2177158..2177676 (+) 519 WP_221763795.1 Holliday junction resolvase RuvX -
  ZMTM_RS10460 (ZMTM_19930) pyrR 2177673..2178176 (+) 504 WP_404804665.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  ZMTM_RS10465 (ZMTM_19940) - 2178176..2179126 (+) 951 WP_221763796.1 aspartate carbamoyltransferase catalytic subunit -
  ZMTM_RS10470 (ZMTM_19950) - 2179126..2180400 (+) 1275 WP_221763797.1 dihydroorotase -
  ZMTM_RS10475 (ZMTM_19960) pilU 2180404..2181540 (-) 1137 WP_221763798.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ZMTM_RS10480 (ZMTM_19970) pilT 2181585..2182628 (-) 1044 WP_221763799.1 type IV pilus twitching motility protein PilT Machinery gene
  ZMTM_RS10485 (ZMTM_19980) - 2182681..2183373 (+) 693 WP_221763800.1 YggS family pyridoxal phosphate-dependent enzyme -
  ZMTM_RS10490 (ZMTM_19990) proC 2183435..2184244 (+) 810 WP_221763801.1 pyrroline-5-carboxylate reductase -
  ZMTM_RS10495 (ZMTM_20000) - 2184250..2184825 (+) 576 WP_221763802.1 YggT family protein -
  ZMTM_RS10500 (ZMTM_20010) - 2185214..2187241 (+) 2028 WP_221763803.1 dynamin family protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38255.78 Da        Isoelectric Point: 6.5015

>NTDB_id=83495 ZMTM_RS10480 WP_221763799.1 2181585..2182628(-) (pilT) [Methyloradius palustris strain Zm11]
MDISDLLAFSVKNKASDLHLSAGLPPMIRVHGDIRKINLPSMDHSEVHDMVYDIMNDGQRKVYEETLECDFSFEIPNLAR
FRVNAFNQNRGSGAVFRTIPSKILSMEQLGCPPIFKEIAQNPRGIVLVTGPTGSGKSTTLAAMVDYVNENEFGHILTVED
PIEFVHTSKKCLINQREVGPHTLSFSNALRSALREDPDVILVGEMRDLETIRLALSAAETGHLVFGTLHTSSAAKTIDRV
VDVFPAAEKEMVRSMLSESLRAVISQTLCKTKDEQGRVAAHEIMIGTPAIRNLIRENKIAQMYSAIQTGQNVGMQTLDQN
LLDLVRRNVISNAEARSKAANKDAFGG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=83495 ZMTM_RS10480 WP_221763799.1 2181585..2182628(-) (pilT) [Methyloradius palustris strain Zm11]
GTGGATATTTCTGATTTACTTGCATTTTCGGTCAAAAATAAAGCATCAGATTTGCATTTATCAGCAGGTTTGCCACCAAT
GATCCGGGTACATGGCGACATCCGTAAAATCAACTTGCCATCAATGGATCACAGTGAAGTACACGACATGGTGTACGACA
TCATGAATGATGGCCAGCGCAAGGTGTATGAAGAAACACTGGAATGTGACTTTTCGTTTGAGATTCCCAATCTTGCACGT
TTCCGCGTCAATGCGTTTAATCAGAATCGCGGCTCTGGTGCGGTATTCCGTACTATTCCTTCCAAGATTCTTTCTATGGA
ACAGCTCGGCTGTCCGCCGATTTTCAAGGAAATCGCACAAAATCCACGCGGCATCGTTCTGGTGACAGGCCCAACTGGTT
CAGGTAAGTCAACCACATTGGCAGCCATGGTTGATTATGTGAATGAAAATGAATTTGGCCATATTCTTACAGTTGAAGAC
CCGATTGAGTTTGTGCATACCTCGAAAAAATGCCTGATCAACCAACGTGAAGTCGGCCCGCACACGCTTTCTTTCTCAAA
TGCCTTGCGTTCAGCGCTGCGTGAAGATCCTGACGTGATTCTGGTTGGCGAAATGCGCGATCTGGAAACTATCCGCTTGG
CGTTAAGCGCTGCGGAAACTGGTCACTTGGTATTCGGTACCTTGCATACCAGTTCAGCTGCCAAAACCATTGACCGCGTG
GTGGATGTGTTCCCAGCCGCCGAGAAGGAAATGGTACGCTCGATGCTTTCAGAATCACTGCGTGCAGTAATCTCGCAAAC
CTTATGTAAAACCAAAGATGAACAAGGTCGAGTTGCAGCGCATGAAATCATGATTGGTACGCCAGCGATTCGTAATTTGA
TACGCGAAAATAAGATTGCGCAGATGTATTCCGCTATTCAAACGGGACAAAACGTTGGTATGCAAACGCTGGATCAAAAC
CTGTTGGATTTGGTGCGTCGTAATGTCATTTCAAATGCTGAAGCGCGTTCCAAGGCCGCTAATAAAGACGCATTTGGTGG
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

77.101

99.424

0.767
  pilT Acinetobacter baumannii D1279779

77.101

99.424

0.767
  pilT Acinetobacter baumannii strain A118

77.101

99.424

0.767
  pilT Pseudomonas aeruginosa PAK

74.783

99.424

0.744
  pilT Pseudomonas stutzeri DSM 10701

74.493

99.424

0.741
  pilT Acinetobacter baylyi ADP1

73.623

99.424

0.732
  pilT Legionella pneumophila strain Lp02

73.623

99.424

0.732
  pilT Legionella pneumophila strain ERS1305867

73.623

99.424

0.732
  pilT Neisseria meningitidis 8013

71.014

99.424

0.706
  pilT Neisseria gonorrhoeae MS11

70.725

99.424

0.703
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.639

97.406

0.669
  pilT Vibrio cholerae strain A1552

68.639

97.406

0.669
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.435

99.424

0.501
  pilU Acinetobacter baylyi ADP1

43.529

97.983

0.427
  pilU Pseudomonas stutzeri DSM 10701

41.194

96.542

0.398
  pilU Vibrio cholerae strain A1552

40.299

96.542

0.389

Multiple sequence alignment