Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   U2S75_RS03040 Genome accession   NZ_CP140420
Coordinates   645064..646554 (+) Length   496 a.a.
NCBI ID   WP_001166322.1    Uniprot ID   -
Organism   Acinetobacter baumannii strain 2023CK-00893     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 640064..651554
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U2S75_RS03025 (U2S75_03025) - 640422..643229 (+) 2808 WP_064192076.1 DUF2339 domain-containing protein -
  U2S75_RS03030 (U2S75_03030) - 643219..644136 (-) 918 WP_000785635.1 mechanosensitive ion channel family protein -
  U2S75_RS03035 (U2S75_03035) - 644146..644967 (-) 822 WP_000462729.1 hypothetical protein -
  U2S75_RS03040 (U2S75_03040) pilB 645064..646554 (+) 1491 WP_001166322.1 type II secretion system ATPase GspE Machinery gene
  U2S75_RS03045 (U2S75_03045) - 646609..647040 (-) 432 WP_000051124.1 organic hydroperoxide resistance protein -
  U2S75_RS03050 (U2S75_03050) - 647128..647565 (-) 438 WP_005112061.1 MarR family winged helix-turn-helix transcriptional regulator -
  U2S75_RS03055 (U2S75_03055) - 647725..648102 (-) 378 WP_000266123.1 DUF4951 domain-containing protein -
  U2S75_RS03060 (U2S75_03060) - 648117..648941 (-) 825 WP_064191938.1 carbon-nitrogen hydrolase family protein -
  U2S75_RS03065 (U2S75_03065) gigB 648968..649489 (-) 522 WP_000103065.1 anti-anti-sigma factor GigB -
  U2S75_RS03070 (U2S75_03070) gigA 649592..650560 (-) 969 WP_000273589.1 RsbU family protein phosphatase GigA -

Sequence

Protein


Download         Length: 496 a.a.        Molecular weight: 55319.18 Da        Isoelectric Point: 6.2037

>NTDB_id=834645 U2S75_RS03040 WP_001166322.1 645064..646554(+) (pilB) [Acinetobacter baumannii strain 2023CK-00893]
MQILKQIQVPYSFAKRHGVLFRYDGDQVFIVRRQNTEKIALQEARRILGKPAHYQLCTEQEFNSLLSTSYAGDTGESQQV
AAGLEDHPDLLSLADQVPETEDLMDQEDDAPIVRLINALLSEAIRVGASDIHIEAFEKKLSVRLRVDGQLREIVQPRREL
APLLVSRIKVMAKLDIAEKRVPQDGRISLRLAGREVDVRVSTLPSSHGERVVMRLLDKQAGRLNMTHLGLMANDYERLTQ
LVHRPHGIILVTGPTGSGKTTTLYAALSDLNDNTRNILTAEDPIEYQLEGIGQTQVNTKVDMTFARALKAMLRQDPDVVM
VGEIRDLETAEIAVQASLTGHLVLSTLHTNTAIGAVTRLKDMGIEPFLLSSSLIGVVAQRLVRTLCSHCMTWREADTFEK
QVFQHISHEPSLKLPEAQGCDQCSHLGFNGRTAIYEIVPIDEPMRRLIHGNAAEFELENHARQYSGSIRDDGLRKVLSGK
TTLEEVLRVTNEASEA

Nucleotide


Download         Length: 1491 bp        

>NTDB_id=834645 U2S75_RS03040 WP_001166322.1 645064..646554(+) (pilB) [Acinetobacter baumannii strain 2023CK-00893]
ATGCAAATATTGAAACAAATACAAGTTCCATATAGTTTTGCCAAGCGTCATGGTGTTTTATTTCGTTATGACGGTGATCA
AGTTTTTATTGTTAGACGTCAAAATACAGAAAAAATTGCATTGCAGGAAGCAAGACGAATTTTGGGGAAACCTGCACACT
ATCAATTATGCACTGAACAAGAATTTAATAGTCTATTAAGTACCAGTTATGCAGGGGATACAGGCGAATCTCAACAAGTA
GCTGCGGGCTTAGAAGACCATCCGGATTTATTAAGCCTTGCAGATCAGGTGCCTGAAACAGAAGACTTAATGGATCAAGA
GGATGACGCGCCTATTGTTCGTCTAATCAATGCACTTCTCTCTGAGGCAATTCGTGTTGGAGCTTCAGATATCCATATTG
AAGCCTTTGAGAAAAAGCTATCAGTTCGTTTACGGGTAGATGGTCAATTACGTGAAATTGTGCAGCCACGCCGTGAACTA
GCCCCGTTACTTGTTTCGCGTATTAAAGTTATGGCGAAACTCGATATTGCCGAAAAGCGTGTACCGCAAGATGGACGTAT
TTCTTTACGTCTTGCTGGTCGTGAAGTCGATGTCCGTGTTTCAACTTTGCCATCGTCACATGGTGAAAGGGTGGTCATGC
GTTTACTCGATAAACAAGCGGGTCGCCTTAATATGACTCACCTAGGTTTAATGGCAAATGACTATGAACGTTTAACTCAG
CTAGTACATCGTCCGCACGGTATTATTTTGGTCACAGGGCCTACTGGTTCGGGGAAAACGACGACTTTATATGCTGCCTT
ATCTGATCTAAATGATAACACCCGTAACATCTTAACTGCCGAAGATCCAATTGAGTATCAGCTTGAAGGTATTGGCCAAA
CTCAAGTGAATACCAAAGTAGATATGACTTTTGCTCGAGCTTTAAAAGCAATGTTACGTCAAGATCCAGATGTGGTCATG
GTAGGGGAGATTCGTGATCTAGAAACTGCTGAAATTGCGGTGCAGGCATCTTTAACAGGTCATTTGGTGTTATCAACACT
ACATACCAATACCGCTATTGGTGCAGTGACTCGACTTAAAGATATGGGGATTGAACCTTTCTTACTTTCAAGCTCTTTAA
TTGGGGTGGTTGCGCAGCGTTTGGTTCGTACTTTATGTTCACATTGTATGACATGGCGTGAAGCCGATACTTTTGAGAAG
CAAGTTTTTCAGCATATTTCCCATGAACCAAGTTTAAAGTTACCAGAGGCTCAAGGGTGTGATCAGTGTTCTCATTTAGG
GTTTAATGGCCGTACAGCGATTTATGAAATTGTACCTATTGATGAACCTATGCGCCGTCTCATTCATGGTAATGCAGCTG
AATTTGAATTAGAAAATCATGCTCGTCAGTATTCTGGTTCTATTCGCGATGATGGTTTACGTAAGGTGCTCTCAGGTAAA
ACTACACTTGAAGAAGTGCTTCGTGTAACCAATGAAGCAAGTGAAGCATAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(118-384)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.26

99.194

0.409
  pilB Legionella pneumophila strain ERS1305867

41.598

98.387

0.409
  pilF Thermus thermophilus HB27

39.351

99.395

0.391
  pilB/pilB1 Synechocystis sp. PCC 6803

40.041

97.177

0.389
  pilF Neisseria gonorrhoeae MS11

48.731

79.435

0.387
  pilB Vibrio cholerae strain A1552

48

80.645

0.387
  pilB Vibrio campbellii strain DS40M4

48.12

80.444

0.387
  pilB Acinetobacter baumannii D1279779

47.395

81.25

0.385
  pilB Vibrio parahaemolyticus RIMD 2210633

47.75

80.645

0.385
  pilB Acinetobacter baylyi ADP1

47.044

78.427

0.369