Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   QMY55_RS03975 Genome accession   NZ_CP125947
Coordinates   874696..876438 (-) Length   580 a.a.
NCBI ID   WP_283487410.1    Uniprot ID   -
Organism   Comamonas resistens strain ZM22     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 869696..881438
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QMY55_RS03945 (QMY55_03945) - 870234..870686 (+) 453 WP_283488877.1 NUDIX domain-containing protein -
  QMY55_RS03950 (QMY55_03950) - 870811..871020 (-) 210 WP_283487406.1 DNA gyrase inhibitor YacG -
  QMY55_RS03955 (QMY55_03955) zapD 871093..871848 (-) 756 WP_283487407.1 cell division protein ZapD -
  QMY55_RS03960 (QMY55_03960) coaE 871934..872560 (-) 627 WP_283488878.1 dephospho-CoA kinase -
  QMY55_RS03965 (QMY55_03965) - 872583..873452 (-) 870 WP_283487408.1 A24 family peptidase -
  QMY55_RS03970 (QMY55_03970) pilC 873452..874669 (-) 1218 WP_283487409.1 type II secretion system F family protein Machinery gene
  QMY55_RS03975 (QMY55_03975) pilB 874696..876438 (-) 1743 WP_283487410.1 type IV-A pilus assembly ATPase PilB Machinery gene
  QMY55_RS03985 (QMY55_03985) - 876689..877618 (-) 930 WP_283487411.1 polyprenyl synthetase family protein -
  QMY55_RS03990 (QMY55_03990) rplU 877850..878161 (+) 312 WP_003058536.1 50S ribosomal protein L21 -
  QMY55_RS03995 (QMY55_03995) rpmA 878178..878435 (+) 258 WP_003058534.1 50S ribosomal protein L27 -
  QMY55_RS04000 (QMY55_04000) cgtA 878591..879706 (+) 1116 WP_283487412.1 Obg family GTPase CgtA -
  QMY55_RS04005 (QMY55_04005) proB 879817..880956 (+) 1140 WP_283487413.1 glutamate 5-kinase -

Sequence

Protein


Download         Length: 580 a.a.        Molecular weight: 63079.19 Da        Isoelectric Point: 5.1523

>NTDB_id=833637 QMY55_RS03975 WP_283487410.1 874696..876438(-) (pilB) [Comamonas resistens strain ZM22]
MAAADSLQKTPPAPIAIPGLGRALISAGKVSQQAAEAAAKKAASSKTPFISELSQSGEISALEIAETISTMFSTPLLDLN
AVDVSQLPRELLDPKISSSYRVLALSKRGNRLTVATADPTQQEAAEQIKFTTQLFVDWVVVEADKLHKLIDQAGKSVGES
LDTYPSSDDFDFDDVKIEDAPEEKDNAIGADVEDAPVVKFLHKMLLDAFNMRASDLHFEPYEHQYRVRFRIDGELREITS
PPIAIKDKLASRIKVISRLDISEKRVPQDGRMKLKVGPDRVIDFRVSTLPTLFGEKIVIRILDPSSAKMGIDALGYEPEE
KERLLSAIKRPYGMILVTGPTGSGKTVSLYTCLNLLNQPGVNIATAEDPSEINMPGVNQVNVNEKAGLTFAAALKSFLRQ
DPDIIMVGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCSQCKQPAD
IPHEALIDAGYSEDEVDGSWVSYKPVGCAACNNGYKGRVGIYQVMPISEEIQRIILRDGSALEIAAQAKAEGVRSLRESG
LHKVKLGLTSLEEVLAVTNE

Nucleotide


Download         Length: 1743 bp        

>NTDB_id=833637 QMY55_RS03975 WP_283487410.1 874696..876438(-) (pilB) [Comamonas resistens strain ZM22]
ATGGCTGCTGCCGATTCGCTGCAAAAAACCCCTCCAGCACCCATCGCTATCCCCGGCCTGGGTCGTGCGCTGATCTCTGC
TGGCAAGGTTTCACAGCAAGCGGCCGAAGCTGCTGCAAAAAAAGCTGCCAGCAGCAAAACCCCGTTTATTTCGGAGCTCA
GTCAATCCGGCGAAATCAGCGCCTTGGAAATCGCCGAGACGATCTCCACCATGTTCAGCACACCTTTGCTGGACCTGAAC
GCCGTGGATGTCTCGCAGCTGCCGCGCGAACTGTTGGATCCCAAGATCAGCAGCAGCTATCGCGTCCTGGCCCTGAGCAA
GCGTGGCAACCGATTGACGGTTGCCACCGCCGACCCCACGCAGCAGGAAGCGGCCGAGCAGATCAAGTTCACCACCCAGC
TGTTCGTGGACTGGGTAGTCGTTGAAGCCGACAAGCTGCACAAGCTCATTGACCAAGCTGGAAAAAGTGTCGGTGAATCC
CTCGATACCTACCCCAGCTCCGACGATTTCGACTTTGATGACGTCAAGATCGAAGATGCTCCCGAGGAAAAAGACAACGC
CATCGGAGCCGATGTAGAGGATGCGCCTGTCGTCAAGTTTCTGCACAAGATGCTGCTTGACGCCTTCAACATGCGGGCAT
CTGACCTGCACTTCGAGCCCTATGAGCACCAGTATCGCGTGCGCTTCCGTATTGACGGCGAGTTGCGCGAAATCACCTCC
CCGCCCATCGCCATCAAGGACAAGCTGGCTTCTCGCATCAAGGTGATCTCACGCCTCGATATTTCCGAAAAGCGTGTGCC
CCAGGACGGCCGCATGAAGCTCAAGGTTGGCCCGGATCGGGTCATCGACTTTCGCGTCAGCACTTTGCCCACCTTGTTTG
GCGAGAAGATCGTGATCCGTATTCTGGATCCCAGCTCCGCCAAGATGGGCATTGATGCCCTGGGCTATGAGCCCGAGGAA
AAGGAACGCCTGCTGTCGGCTATCAAGCGCCCTTACGGCATGATTCTGGTCACAGGCCCCACAGGTTCCGGCAAGACCGT
GTCGCTCTATACCTGCCTGAACCTGCTCAATCAGCCCGGTGTGAACATTGCGACCGCAGAAGACCCTTCCGAAATCAATA
TGCCGGGGGTCAACCAGGTCAATGTGAACGAAAAGGCCGGTCTGACCTTTGCGGCGGCTCTCAAGTCCTTTCTGCGTCAG
GATCCGGACATCATCATGGTGGGTGAAATTCGGGATCTGGAAACCGCCGACATTTCCATCAAGGCTGCGCAGACCGGCCA
CCTGGTGCTGTCAACGCTGCACACCAACGACGCCCCGACAACGCTCACGCGCATGCGCAATATGGGCATTGCGCCCTTCA
ATATTGCATCCAGCGTGATTCTGATCACGGCCCAGCGGCTGGCGAGACGTTTGTGCTCGCAGTGCAAGCAGCCGGCCGAT
ATACCGCATGAAGCCTTGATCGATGCCGGCTATAGCGAGGACGAAGTGGATGGCAGCTGGGTGAGTTACAAGCCCGTAGG
TTGCGCCGCCTGCAACAATGGCTACAAGGGACGTGTTGGTATCTATCAGGTCATGCCCATCAGCGAGGAAATTCAGCGCA
TCATTTTGCGCGACGGCAGTGCGCTAGAAATCGCCGCACAAGCCAAGGCCGAAGGTGTACGCTCTCTGCGCGAATCCGGT
CTGCACAAAGTGAAATTAGGCCTGACATCACTCGAAGAAGTGCTGGCCGTCACCAACGAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(203-470)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(55-155)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

54.671

99.655

0.545
  pilB Acinetobacter baumannii D1279779

55.062

97.069

0.534
  pilF Neisseria gonorrhoeae MS11

52.12

97.586

0.509
  pilB Legionella pneumophila strain ERS1305867

50.177

97.586

0.49
  pilB Vibrio cholerae strain A1552

46.774

96.207

0.45
  pilB Vibrio campbellii strain DS40M4

44.544

96.379

0.429
  pilB Vibrio parahaemolyticus RIMD 2210633

44.544

96.379

0.429
  pilF Thermus thermophilus HB27

37.925

100

0.384
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.421

98.276

0.378