Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   U0543_RS07665 Genome accession   NZ_CP139836
Coordinates   1573827..1575317 (-) Length   496 a.a.
NCBI ID   WP_029423664.1    Uniprot ID   -
Organism   Acinetobacter baumannii strain B84     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1568827..1580317
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U0543_RS07635 (U0543_07635) gigA 1569818..1570786 (+) 969 WP_000273589.1 RsbU family protein phosphatase GigA -
  U0543_RS07640 (U0543_07640) gigB 1570889..1571410 (+) 522 WP_000103065.1 anti-anti-sigma factor GigB -
  U0543_RS07645 (U0543_07645) - 1571437..1572261 (+) 825 WP_000177091.1 carbon-nitrogen hydrolase family protein -
  U0543_RS07650 (U0543_07650) - 1572276..1572653 (+) 378 WP_000266123.1 DUF4951 domain-containing protein -
  U0543_RS07655 (U0543_07655) - 1572814..1573251 (+) 438 WP_000371731.1 MarR family winged helix-turn-helix transcriptional regulator -
  U0543_RS07660 (U0543_07660) - 1573339..1573770 (+) 432 WP_000051124.1 organic hydroperoxide resistance protein -
  U0543_RS07665 (U0543_07665) pilB 1573827..1575317 (-) 1491 WP_029423664.1 type II secretion system ATPase GspE Machinery gene
  U0543_RS07670 (U0543_07670) - 1575414..1576235 (+) 822 WP_029423663.1 hypothetical protein -
  U0543_RS07675 (U0543_07675) - 1576245..1577162 (+) 918 WP_057078051.1 mechanosensitive ion channel family protein -
  U0543_RS07680 (U0543_07680) - 1577152..1579956 (-) 2805 WP_135651855.1 DUF2339 domain-containing protein -

Sequence

Protein


Download         Length: 496 a.a.        Molecular weight: 55396.36 Da        Isoelectric Point: 6.2037

>NTDB_id=831808 U0543_RS07665 WP_029423664.1 1573827..1575317(-) (pilB) [Acinetobacter baumannii strain B84]
MQILKQIQVPYSFAKRHGVLFRYDGDQVFIVRRQNTEKIALQEARRILGKPAHYQLCTEQEFNSLLSTSYAGDTGESQQV
AAGLEDHPDLLSLADQVPETEDLMDQEDDAPIVRLINALLSEAIRVGASDIHIEAFEKKLSVRLRVDGQLREIVQPRREL
APLLVSRIKVMAKLDIAEKRVPQDGRISLRLAGREVDVRVSTLPSSHGERVVMRLLDKQAGRLNMTHLGLMANDYERLTQ
LVHRPHGIILVTGPTGSGKTTTLYAALSDLNDNTRNILTAEDPIEYQLEGIGQTQVNTKVDMTFARALKAMLRQDPDVVM
VGEIRDLETAEIAVQASLTGHLVLSTLHTNTAIGAVTRLKDMGIEPFLLSSSLIGVVAQRLVRTLCPHCMTWREADTFEK
QVFQHISHEPTLKIPEAQGCDQCSHLGFNGRTAIYEIVPIDEPMRRLIHGNAAEFELENHARQYSGSIRDDGLRKVILGK
TTLEEVLRVTNEANEA

Nucleotide


Download         Length: 1491 bp        

>NTDB_id=831808 U0543_RS07665 WP_029423664.1 1573827..1575317(-) (pilB) [Acinetobacter baumannii strain B84]
ATGCAAATATTGAAACAAATACAAGTTCCATATAGTTTTGCCAAGCGTCATGGTGTTTTATTTCGTTATGACGGTGATCA
AGTTTTTATTGTTAGACGTCAAAATACAGAAAAAATTGCATTGCAGGAAGCAAGACGAATTTTGGGGAAGCCTGCACATT
ATCAGCTATGTACTGAACAAGAGTTTAATAGCCTATTAAGTACGAGTTATGCGGGGGACACAGGCGAATCTCAACAAGTT
GCGGCTGGCTTAGAAGACCACCCGGATTTATTGAGTTTGGCCGATCAGGTTCCTGAAACAGAAGATTTAATGGATCAGGA
AGATGACGCGCCTATTGTGCGTCTGATCAATGCACTTCTCTCTGAGGCAATCCGTGTAGGTGCTTCAGATATTCATATTG
AAGCTTTTGAAAAGAAATTATCTGTACGTTTACGGGTAGATGGTCAATTACGCGAGATTGTACAGCCACGCCGTGAACTA
GCTCCCTTACTTGTTTCACGTATTAAAGTTATGGCAAAACTCGATATTGCCGAAAAGCGTGTACCGCAAGATGGACGTAT
TTCTTTGCGTCTTGCTGGCCGTGAAGTTGATGTCCGTGTTTCAACTTTGCCATCGTCACATGGTGAAAGGGTTGTTATGC
GTTTGCTTGATAAACAAGCGGGTCGCCTTAATATGACTCACCTAGGTTTAATGGCAAATGACTATGAACGTTTAACTCAG
TTAGTACATCGTCCGCACGGTATTATTTTAGTCACAGGACCTACTGGTTCGGGGAAAACGACGACTTTATATGCTGCCTT
ATCAGATCTAAATGACAATACCCGTAACATCTTAACAGCCGAAGATCCAATTGAATATCAGCTTGAAGGTATTGGCCAAA
CTCAAGTGAATACCAAAGTGGATATGACTTTTGCTCGAGCTTTAAAGGCAATGTTACGTCAAGATCCAGATGTGGTCATG
GTCGGTGAGATTCGTGATTTAGAAACTGCTGAAATTGCGGTGCAGGCATCTTTAACAGGTCATTTGGTGTTATCAACACT
ACATACCAATACTGCTATTGGTGCAGTGACTCGACTTAAAGATATGGGGATTGAACCCTTCCTATTATCAAGTTCTTTAA
TTGGGGTCGTTGCTCAGCGTTTGGTTCGTACCTTATGTCCACATTGTATGACATGGCGTGAAGCCGATACTTTTGAAAAG
CAGGTTTTTCAGCATATTTCGCATGAGCCAACTTTAAAGATACCAGAAGCTCAAGGGTGTGATCAGTGTTCTCATTTAGG
GTTTAATGGCCGTACGGCTATTTATGAAATTGTACCTATTGATGAACCGATGCGTCGTCTTATTCATGGCAATGCAGCTG
AGTTTGAATTAGAAAACCATGCTCGCCAGTACTCGGGTTCTATTCGTGATGATGGCTTGCGCAAGGTAATTTTAGGTAAA
ACCACACTTGAAGAAGTATTGCGTGTAACCAATGAAGCAAATGAAGCATAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(118-384)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

41.393

98.387

0.407
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.65

99.194

0.403
  pilB Vibrio campbellii strain DS40M4

48.872

80.444

0.393
  pilB Vibrio parahaemolyticus RIMD 2210633

48.25

80.645

0.389
  pilB/pilB1 Synechocystis sp. PCC 6803

40.208

96.774

0.389
  pilF Thermus thermophilus HB27

38.945

99.395

0.387
  pilB Vibrio cholerae strain A1552

47.859

80.04

0.383
  pilF Neisseria gonorrhoeae MS11

48.223

79.435

0.383
  pilB Acinetobacter baumannii D1279779

47.146

81.25

0.383
  pilB Acinetobacter baylyi ADP1

47.044

78.427

0.369