Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   QMK51_RS04585 Genome accession   NZ_CP125375
Coordinates   991332..993032 (-) Length   566 a.a.
NCBI ID   WP_320393279.1    Uniprot ID   -
Organism   Pseudomonas sp. P9_31     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 986332..998032
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QMK51_RS04555 (QMK51_04560) - 987444..988133 (+) 690 WP_320393275.1 energy-coupling factor ABC transporter permease -
  QMK51_RS04560 (QMK51_04565) - 988197..988412 (+) 216 WP_258699036.1 hypothetical protein -
  QMK51_RS04565 (QMK51_04570) yacG 988421..988621 (-) 201 WP_223485972.1 DNA gyrase inhibitor YacG -
  QMK51_RS04570 (QMK51_04575) coaE 988618..989241 (-) 624 WP_320393276.1 dephospho-CoA kinase -
  QMK51_RS04575 (QMK51_04580) pilD 989238..990110 (-) 873 WP_320393277.1 A24 family peptidase Machinery gene
  QMK51_RS04580 (QMK51_04585) pilC 990112..991329 (-) 1218 WP_320393278.1 type II secretion system F family protein Machinery gene
  QMK51_RS04585 (QMK51_04590) pilB 991332..993032 (-) 1701 WP_320393279.1 type IV-A pilus assembly ATPase PilB Machinery gene
  QMK51_RS04590 (QMK51_04595) - 993259..993669 (+) 411 WP_320393280.1 pilin -
  QMK51_RS04595 (QMK51_04600) - 993991..994347 (+) 357 WP_320393281.1 BON domain-containing protein -
  QMK51_RS04600 (QMK51_04605) - 994632..994928 (-) 297 WP_320393282.1 DUF2845 domain-containing protein -
  QMK51_RS04605 (QMK51_04610) - 994928..997924 (-) 2997 WP_320393283.1 DUF748 domain-containing protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62990.91 Da        Isoelectric Point: 5.6733

>NTDB_id=831538 QMK51_RS04585 WP_320393279.1 991332..993032(-) (pilB) [Pseudomonas sp. P9_31]
MNDIALSGLAKQLVLAELITDQSAQQAYQQAQRSRISFVSYLVQNKLLKSWQVAEVASDHFGMALLDLNCLDKETQPRGL
VSEKLIRQHHALPLWRRGNKLFVGISDPTNHQAINDIQFSTGLSTEAILVEEDKLNDAIEKFFDNHSTGLEDMADVDLDG
LDIESVDDQKQDSIAGQDTDDAPVVRFVHKMLLDAIKSGSSDLHFEPYEKTYRVRMRTDGILHEVAKPPTQLANRIASRL
KVMASLDISERRKPQDGRIKMRLSKSKSIDFRVNTLPTLWGEKVVIRILDPSSAQMGIDALGYEPDQKDLYMAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPRQGMDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHMVLSTLHTNSASQTLTRLHNMGVQGFNIATSVSLIIAQRLARKLCSHCKKPIEIPREALLKEGFPE
ERIGSFTIYEPVGCDQCNNGYKGRVGIYEVVKNTPDLQRLIMAEGNSLEIDLQMRKDGFNDLRTSGLLKAMQGITSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=831538 QMK51_RS04585 WP_320393279.1 991332..993032(-) (pilB) [Pseudomonas sp. P9_31]
ATGAATGACATCGCCCTTAGCGGTCTGGCCAAGCAATTGGTACTGGCCGAGCTGATCACTGACCAAAGTGCGCAACAGGC
GTATCAGCAAGCCCAACGCAGTCGAATCTCCTTCGTCAGCTATCTGGTGCAGAACAAACTGCTGAAAAGCTGGCAGGTCG
CCGAAGTCGCTTCAGATCATTTCGGCATGGCCCTGCTGGACCTCAATTGCCTGGACAAGGAAACCCAACCCCGTGGGCTG
GTCAGCGAAAAGCTGATCCGTCAGCACCACGCCCTGCCTCTCTGGCGGCGTGGCAACAAGCTGTTCGTGGGGATTTCCGA
CCCGACCAATCACCAGGCCATCAATGACATCCAGTTCAGCACCGGGCTGAGCACCGAAGCCATCCTGGTGGAGGAAGACA
AGCTCAATGATGCCATCGAGAAATTCTTCGACAACCACTCCACCGGCCTGGAAGACATGGCCGATGTCGACCTCGACGGC
CTGGACATCGAGTCGGTCGATGACCAGAAACAGGATTCCATCGCAGGGCAAGATACCGATGACGCGCCCGTGGTGCGCTT
CGTCCACAAGATGTTGCTCGATGCGATCAAGAGCGGTTCTTCCGACCTGCATTTCGAGCCCTACGAAAAAACCTACCGCG
TGCGGATGCGCACCGATGGCATCCTGCATGAGGTCGCCAAGCCACCGACACAACTGGCCAATCGCATCGCTTCCCGGCTG
AAAGTCATGGCCAGCCTCGACATCTCCGAACGGCGCAAACCCCAGGACGGGCGAATCAAGATGCGCTTGTCCAAAAGCAA
GTCCATCGACTTCCGGGTCAACACCCTGCCGACCCTGTGGGGCGAGAAAGTGGTGATCCGGATCCTCGACCCATCCAGTG
CCCAAATGGGCATCGACGCCCTCGGCTATGAGCCCGACCAGAAAGACCTGTACATGGCCGCCCTCAAGCAGCCACAGGGG
ATGATCCTGGTGACCGGCCCCACCGGCTCAGGCAAGACCGTGTCGCTCTATACCGGTCTGAACATCCTCAACACCGTCGA
CATCAACATCTCCACTGCCGAAGACCCGGTAGAAATCAATATGGAAGGCATCAACCAGGTCAACGTCAATCCCAGGCAGG
GAATGGATTTCGCCCAGGCGCTGCGCTCGTTTCTGCGCCAGGACCCGGACGTGATCATGGTCGGTGAAATTCGCGACCTC
GAAACCGCCGAAATCGCCATCAAGGCCGCCCAAACCGGGCACATGGTGCTTTCCACCCTGCACACCAACAGCGCTTCGCA
AACCCTGACCCGCCTGCACAACATGGGGGTTCAGGGCTTCAACATCGCGACCTCGGTCAGCCTGATCATCGCCCAGCGCC
TGGCGCGCAAACTGTGCAGTCATTGCAAGAAACCCATCGAAATTCCCCGCGAGGCGCTGCTCAAGGAAGGCTTCCCCGAG
GAACGCATCGGCTCGTTCACGATCTATGAACCGGTCGGTTGCGATCAGTGCAACAACGGTTACAAAGGGCGAGTAGGCAT
TTATGAAGTGGTCAAGAACACACCGGATTTGCAACGGCTGATCATGGCCGAAGGCAACTCACTGGAAATCGACCTCCAGA
TGCGCAAGGACGGCTTCAATGACCTGCGCACTTCGGGCCTGCTCAAGGCCATGCAAGGCATCACCAGCCTTGAAGAAATC
AACCGGGTCACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.333

99.647

0.581
  pilB Acinetobacter baylyi ADP1

57.904

99.47

0.576
  pilB Legionella pneumophila strain ERS1305867

55.654

100

0.557
  pilB Vibrio cholerae strain A1552

51.15

99.823

0.511
  pilB Vibrio campbellii strain DS40M4

48.596

100

0.489
  pilF Neisseria gonorrhoeae MS11

49.023

99.47

0.488
  pilB Vibrio parahaemolyticus RIMD 2210633

49.281

98.233

0.484