Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   QMK48_RS24055 Genome accession   NZ_CP125374
Coordinates   5411941..5413641 (+) Length   566 a.a.
NCBI ID   WP_258689399.1    Uniprot ID   -
Organism   Pseudomonas sp. P9_32     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5406941..5418641
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QMK48_RS24035 (QMK48_24030) - 5407107..5410040 (+) 2934 WP_258689396.1 DUF748 domain-containing protein -
  QMK48_RS24040 (QMK48_24035) - 5410042..5410338 (+) 297 WP_253469448.1 DUF2845 domain-containing protein -
  QMK48_RS24045 (QMK48_24040) - 5410687..5411040 (-) 354 WP_003205664.1 BON domain-containing protein -
  QMK48_RS24050 (QMK48_24045) - 5411309..5411716 (-) 408 WP_214512613.1 pilin -
  QMK48_RS24055 (QMK48_24050) pilB 5411941..5413641 (+) 1701 WP_258689399.1 type IV-A pilus assembly ATPase PilB Machinery gene
  QMK48_RS24060 (QMK48_24055) pilC 5413644..5414861 (+) 1218 WP_214512611.1 type II secretion system F family protein Machinery gene
  QMK48_RS24065 (QMK48_24060) pilD 5414863..5415732 (+) 870 WP_253469458.1 prepilin peptidase Machinery gene
  QMK48_RS24070 (QMK48_24065) coaE 5415732..5416355 (+) 624 WP_258689400.1 dephospho-CoA kinase -
  QMK48_RS24075 (QMK48_24070) yacG 5416352..5416558 (+) 207 WP_214512608.1 DNA gyrase inhibitor YacG -
  QMK48_RS24080 (QMK48_24075) - 5416555..5416770 (-) 216 WP_053125334.1 hypothetical protein -
  QMK48_RS24085 (QMK48_24080) - 5416834..5417520 (-) 687 WP_258689401.1 energy-coupling factor ABC transporter permease -
  QMK48_RS24090 (QMK48_24085) - 5417558..5417971 (-) 414 WP_253469466.1 tautomerase family protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62687.71 Da        Isoelectric Point: 5.3247

>NTDB_id=831512 QMK48_RS24055 WP_258689399.1 5411941..5413641(+) (pilB) [Pseudomonas sp. P9_32]
MNDIALSGLTKQLVLAELLTEQSAQQAYQQAQRSRIPLVSYLVQNKLVQSRQVAEIASEHFGVALLDLNSLDKETQPSGL
VSEKLVRQHHALPLWRRGNKLYVGISDPTNHQAINDIQFSTGLTTEAILVEDEKLSEAIEKFFESSSTGLEGMGDVDLDG
LDIESIDDTKQDAIGSQDADDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKIYRVRVRTDGMLREVARPPIQLANRIAARL
KVMASLDISERRKPQDGRLKMRLSKTKSIDFRVNTLPTLWGEKVVIRILDPSSAQMGIDALGYEPDQKDLYMAALKQPQG
LILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPRQGLDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLIRLQNMGIPGFNIATAVHLIIAQRLARKLCSHCKKAIEVPEETLLKEGFPR
ERIGTFTIYEPVGCEQCNNGYKGRVGVYEVVKNTPELQRLIMAEGNSLEIDLQMRKDGFNDLRTSGLLKVMQGVTSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=831512 QMK48_RS24055 WP_258689399.1 5411941..5413641(+) (pilB) [Pseudomonas sp. P9_32]
ATGAATGACATCGCCCTCAGCGGCCTGACCAAGCAATTGGTGTTGGCCGAACTGCTCACCGAGCAAAGTGCGCAACAGGC
GTATCAACAGGCTCAACGCAGCCGCATCCCCCTGGTCAGCTATCTGGTGCAGAACAAACTGGTCCAGAGCCGCCAGGTCG
CGGAGATTGCCTCGGAGCATTTCGGCGTCGCCCTGCTGGACCTCAACAGCCTGGACAAAGAAACCCAACCCAGCGGCCTG
GTCAGCGAAAAACTGGTGCGCCAGCACCACGCCCTGCCGCTGTGGCGGCGTGGCAACAAACTGTACGTGGGCATCTCCGA
CCCCACTAATCACCAGGCCATCAACGACATCCAGTTCAGCACTGGCCTGACCACCGAAGCCATCCTGGTGGAGGACGAAA
AGCTCAGCGAGGCCATCGAAAAGTTCTTCGAATCCAGCAGCACCGGCCTGGAAGGCATGGGCGATGTCGACCTCGACGGC
CTGGACATCGAATCGATCGACGACACCAAGCAGGATGCCATCGGCAGCCAGGACGCCGACGACGCGCCCGTGGTGCGCTT
CGTCAACAAGATGTTGCTGGACGCCATCAAGGGCGGCTCTTCCGACCTGCACTTCGAGCCCTATGAAAAGATCTATCGGG
TGCGGGTGCGCACCGACGGCATGCTGCGGGAAGTGGCCCGCCCCCCGATCCAACTGGCCAACCGTATCGCCGCCCGCCTG
AAAGTCATGGCCAGCCTGGATATTTCCGAGCGGCGCAAACCCCAGGACGGCCGGTTGAAAATGCGCCTGTCAAAAACCAA
ATCCATCGACTTCCGGGTCAACACCCTGCCCACGCTGTGGGGCGAAAAGGTGGTGATCCGGATCCTTGATCCGTCCAGTG
CGCAAATGGGCATCGATGCCCTGGGCTACGAACCGGACCAGAAAGACCTGTACATGGCCGCCCTCAAGCAACCGCAAGGG
CTGATCCTGGTGACCGGCCCGACCGGCTCGGGCAAGACCGTGTCGCTGTACACCGGGCTGAATATCCTCAACACCGTGGA
CATCAACATTTCCACCGCCGAAGACCCGGTGGAGATCAACATGGAAGGCATCAACCAGGTCAACGTGAACCCGCGCCAGG
GGTTGGACTTCGCCCAGGCCCTGCGTTCGTTCCTGCGCCAGGACCCGGACGTGATCATGGTCGGCGAGATCCGCGACCTG
GAAACCGCCGAGATCGCCATCAAGGCCGCTCAGACCGGGCACCTGGTGCTCTCCACCCTGCACACCAACAGCGCCGCCGA
AACCCTGATCCGCCTGCAGAACATGGGCATTCCAGGGTTCAACATCGCCACCGCCGTGCACCTGATCATCGCCCAGCGGC
TGGCGCGCAAGCTGTGCAGTCACTGCAAGAAAGCCATCGAGGTTCCCGAGGAAACCTTGCTCAAGGAAGGTTTCCCTCGG
GAACGCATTGGCACATTCACGATCTATGAGCCGGTCGGTTGCGAACAGTGCAACAACGGCTACAAAGGGCGCGTGGGGGT
TTACGAAGTGGTCAAGAACACGCCCGAGCTGCAACGGTTGATCATGGCCGAGGGCAACTCGCTGGAAATCGATCTGCAAA
TGCGCAAGGACGGTTTCAATGACCTGCGCACGTCGGGGCTGCTCAAAGTGATGCAGGGCGTCACCAGCCTCGAAGAAATC
AACCGGGTCACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

58.304

100

0.583
  pilB Acinetobacter baumannii D1279779

57.672

100

0.578
  pilB Legionella pneumophila strain ERS1305867

55.458

100

0.557
  pilB Vibrio cholerae strain A1552

50.442

99.823

0.504
  pilB Vibrio campbellii strain DS40M4

48.596

100

0.489
  pilF Neisseria gonorrhoeae MS11

48.313

99.47

0.481
  pilB Vibrio parahaemolyticus RIMD 2210633

48.921

98.233

0.481
  pilF Thermus thermophilus HB27

36.219

100

0.362