Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   QMK48_RS24060 Genome accession   NZ_CP125374
Coordinates   5413644..5414861 (+) Length   405 a.a.
NCBI ID   WP_214512611.1    Uniprot ID   -
Organism   Pseudomonas sp. P9_32     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5408644..5419861
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QMK48_RS24040 (QMK48_24035) - 5410042..5410338 (+) 297 WP_253469448.1 DUF2845 domain-containing protein -
  QMK48_RS24045 (QMK48_24040) - 5410687..5411040 (-) 354 WP_003205664.1 BON domain-containing protein -
  QMK48_RS24050 (QMK48_24045) - 5411309..5411716 (-) 408 WP_214512613.1 pilin -
  QMK48_RS24055 (QMK48_24050) pilB 5411941..5413641 (+) 1701 WP_258689399.1 type IV-A pilus assembly ATPase PilB Machinery gene
  QMK48_RS24060 (QMK48_24055) pilC 5413644..5414861 (+) 1218 WP_214512611.1 type II secretion system F family protein Machinery gene
  QMK48_RS24065 (QMK48_24060) pilD 5414863..5415732 (+) 870 WP_253469458.1 prepilin peptidase Machinery gene
  QMK48_RS24070 (QMK48_24065) coaE 5415732..5416355 (+) 624 WP_258689400.1 dephospho-CoA kinase -
  QMK48_RS24075 (QMK48_24070) yacG 5416352..5416558 (+) 207 WP_214512608.1 DNA gyrase inhibitor YacG -
  QMK48_RS24080 (QMK48_24075) - 5416555..5416770 (-) 216 WP_053125334.1 hypothetical protein -
  QMK48_RS24085 (QMK48_24080) - 5416834..5417520 (-) 687 WP_258689401.1 energy-coupling factor ABC transporter permease -
  QMK48_RS24090 (QMK48_24085) - 5417558..5417971 (-) 414 WP_253469466.1 tautomerase family protein -
  QMK48_RS24095 (QMK48_24090) - 5418123..5419022 (+) 900 WP_258689402.1 LysR substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44228.02 Da        Isoelectric Point: 9.9531

>NTDB_id=831513 QMK48_RS24060 WP_214512611.1 5413644..5414861(+) (pilC) [Pseudomonas sp. P9_32]
MAVKAVKTDVYTWEGKDRKGTKMSGELTGQSPALVKAQLRKQGINPQKVRKKSTSIFSKGKRIKPLDIALFTRQMATMLK
AGVPLLQAFDIIGEGFDNPNMRKLVDEVKQEVAAGNSFAAALRKCPQYFDELYCNLVDAGEQAGALDTLLDRVATYKEKS
EALKAKIKKAMTYPAAVVLVAAVVTGILLVKVVPQFESVFSGFGAQLPAFTVMVIGLSEIMQQWWWVMLGGLVGAFFGVK
YALRRSEPFRDWRDKWLLKLPLIGTLLYKSAVARYARTLSTTFAAGVPLVEALDSVSGATGNVVFKRAVLRIKQEVSTGM
QLNFSMRASGIFPNLAIQMTAIGEESGALDDMLDKVASFYEAEVDNLVDNLTSLMEPFIMVVLGVVVGGLVVAMYLPIFQ
LGSAI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=831513 QMK48_RS24060 WP_214512611.1 5413644..5414861(+) (pilC) [Pseudomonas sp. P9_32]
ATGGCGGTCAAGGCAGTCAAAACCGATGTTTACACTTGGGAAGGCAAGGACCGCAAAGGCACAAAAATGAGTGGCGAGCT
GACGGGCCAGAGCCCGGCGCTGGTCAAGGCACAACTGCGCAAACAAGGCATCAACCCGCAGAAGGTGCGCAAGAAATCCA
CCTCGATATTCAGCAAGGGCAAGCGCATCAAGCCGCTGGATATCGCCCTCTTCACCCGCCAGATGGCCACGATGCTCAAG
GCTGGTGTGCCGCTGTTGCAGGCGTTCGACATCATTGGCGAAGGCTTCGACAACCCCAACATGCGCAAGCTGGTGGACGA
GGTGAAGCAGGAAGTCGCCGCCGGCAACAGCTTCGCCGCGGCGCTGCGCAAGTGCCCGCAATATTTCGACGAGCTGTACT
GCAACCTGGTGGACGCCGGCGAACAGGCCGGTGCCCTGGACACGCTGCTGGACCGGGTCGCGACCTACAAGGAAAAGAGC
GAAGCGCTCAAGGCCAAAATCAAGAAAGCCATGACCTACCCTGCGGCCGTGGTCCTCGTTGCCGCAGTGGTCACGGGGAT
CCTCTTGGTCAAGGTTGTGCCGCAATTCGAATCGGTATTCTCCGGGTTCGGCGCGCAGCTGCCAGCCTTCACGGTAATGG
TCATAGGCCTGTCGGAAATCATGCAGCAATGGTGGTGGGTGATGCTTGGCGGGTTGGTAGGGGCATTTTTTGGCGTGAAG
TACGCGCTCAGGCGTTCGGAGCCCTTTCGCGACTGGCGCGACAAATGGCTGCTCAAGCTGCCGCTGATAGGCACCTTGCT
GTACAAATCCGCCGTGGCCCGCTACGCCCGCACGCTTTCCACCACCTTCGCCGCCGGCGTGCCGCTGGTGGAGGCCCTGG
ATTCGGTGTCTGGTGCCACCGGCAATGTGGTGTTCAAACGTGCCGTGCTGCGCATCAAGCAGGAGGTCTCGACCGGCATG
CAGTTGAATTTCTCCATGCGTGCCTCAGGGATCTTTCCGAACCTGGCGATCCAGATGACCGCCATCGGCGAGGAATCCGG
CGCGCTGGACGACATGCTCGACAAGGTGGCGAGCTTTTATGAGGCCGAGGTGGACAATCTGGTGGACAACCTCACCAGCC
TGATGGAACCCTTCATCATGGTGGTCCTGGGGGTGGTCGTCGGTGGCCTGGTGGTCGCCATGTACTTGCCCATCTTTCAA
CTCGGCTCTGCGATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

75.309

100

0.753
  pilC Acinetobacter baylyi ADP1

59.548

98.272

0.585
  pilC Acinetobacter baumannii D1279779

57.843

100

0.583
  pilC Legionella pneumophila strain ERS1305867

54.798

97.778

0.536
  pilC Vibrio campbellii strain DS40M4

44.192

97.778

0.432
  pilG Neisseria gonorrhoeae MS11

42.786

99.259

0.425
  pilC Vibrio cholerae strain A1552

43.434

97.778

0.425
  pilG Neisseria meningitidis 44/76-A

42.289

99.259

0.42
  pilC Thermus thermophilus HB27

37.157

99.012

0.368