Detailed information NTG0830451

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: OH685_RS07090
• Genome accession: NZ_CP125227
• Coordinates: 1474541..1475659 (-)
• Length: 372 a.a.
• NCBI ID: WP_060465360.1
• Uniprot ID: A0A2I6LWN2
• Organism: Acinetobacter pittii strain SO1
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1469541..1480659

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
OH685_RS07060 (OH685_07125)	-	1470369..1471217 (+)	849	WP_057062207.1	class II glutamine amidotransferase	-
OH685_RS07065 (OH685_07135)	-	1471469..1471924 (+)	456	WP_002121698.1	bacteriohemerythrin	-
OH685_RS07070 (OH685_07140)	-	1472078..1473145 (+)	1068	WP_031944146.1	membrane protein	-
OH685_RS07075 (OH685_07145)	-	1473145..1473459 (+)	315	WP_068614715.1	RnfH family protein	-
OH685_RS07080 (OH685_07150)	omlA	1473524..1473922 (-)	399	WP_002121740.1	outer membrane protein assembly factor BamE	-
OH685_RS07085 (OH685_07155)	fur	1474034..1474471 (+)	438	WP_002121758.1	ferric iron uptake transcriptional regulator	-
OH685_RS07090 (OH685_07160)	pilU	1474541..1475659 (-)	1119	WP_060465360.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
OH685_RS07095 (OH685_07165)	pilT	1475679..1476716 (-)	1038	WP_002121749.1	type IV pilus twitching motility protein PilT	Machinery gene
OH685_RS07100 (OH685_07170)	yggS	1476843..1477535 (+)	693	WP_282463961.1	YggS family pyridoxal phosphate-dependent enzyme	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41917.12 Da
• Isoelectric Point: 6.6403

>NTDB_id=830451 OH685_RS07090 WP_060465360.1 1474541..1475659(-) (pilU) [Acinetobacter pittii strain SO1]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEIIGQLLHSIMSEKQRKEFAETRECNFAIMNRDKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISYRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKSGQITYKDALKHADSPNDLRLTIKLAEEGPDQIAGTNQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=830451 OH685_RS07090 WP_060465360.1 1474541..1475659(-) (pilU) [Acinetobacter pittii strain SO1]
ATGGATTTTAATGACCTGCTCAACCTCATGGTTGAAAAAAAATCATCCGACCTCTTTATTACAGATGGCGTTGCACCATC
GATGAAGATTAACGGGCAAATTGTTCCCATTTCAAAAAATAGTCTTTCAGGCGAAATTATTGGACAACTATTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAGACTCGTGAATGTAATTTTGCCATTATGAACCGCGATAAAACTGCC
CGCTTTCGTGTGAGTGCTTTTCAACAGCGTGACATGCCCGGCATGGTACTACGTCGAATTGAAACTAAAATTCCTTCAAT
TGATGACTTGCAGTTACCACCTGTGCTTAAAGATTTATCGATGACCAAACGCGGCATCATTATTTTTGTAGGTGCAACAG
GTACAGGTAAATCGACATCATTGGCTTCAATGATTAGCTATCGTAACCATAACTCTAAAGGTCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCATGAACACGCTGGCTGCATCATTACCCAACGTGAAGTCGGGATTGATACAGACTCATTTGA
AATTGCTTTAAAGAATACGTTGCGACAAGCACCCGATGTAATCTTGATTGGTGAGATTCGCTCTCGTGAAGTTATGGACT
ACGCGATTGGCTTCGCCGAAACGGGACACTTGGTCTTAGCCACAATGCATGCAAACAATGCCAACCAAGCACTTGACCGT
ATTATTCATTTCTTTGAAAGTGACCGCCACAGTCAGCTCTACATGGACTTATCTCTTAACTTAAAGGCCATGATTGCACA
GCAACTTATTCCGACTCCAGATGGTAATTCGCGCCGTGCAGCGATTGAGATTCTCATTAACTCACCACTAATTTCAGACT
ATATTCGCAAAGGCGAAATTCATGAAATTAAAGATTTAATGAAACGCTCACGTGAACTCGGTATGCAAACTTTTGACCAA
GCACTGTTTGATCTTTATAAGTCAGGCCAAATTACCTATAAAGATGCATTAAAACATGCCGACTCACCAAACGATTTGCG
TTTAACAATTAAGCTTGCCGAAGAAGGACCTGATCAAATTGCAGGCACAAATCAGCACTTAACCTTCGACCGACAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (33-270)

3D structure

• AlphaFold DB: A0A2I6LWN2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.397	100	0.766
pilU	Pseudomonas stutzeri DSM 10701	68.464	99.731	0.683
pilU	Vibrio cholerae strain A1552	54.054	99.462	0.538
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.934	97.849	0.401
pilT	Legionella pneumophila strain Lp02	39.941	90.86	0.363
pilT	Legionella pneumophila strain ERS1305867	39.941	90.86	0.363