Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA   Type   Machinery gene
Locus tag   H7678_RS00725 Genome accession   NZ_AP023393
Coordinates   119034..119975 (+) Length   313 a.a.
NCBI ID   WP_003062163.1    Uniprot ID   -
Organism   Streptococcus dysgalactiae subsp. equisimilis strain 89     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 114034..124975
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H7678_RS00715 (SDSE89_00940) rpoC 114744..118385 (+) 3642 WP_015016542.1 DNA-directed RNA polymerase subunit beta' -
  H7678_RS00720 (SDSE89_00950) - 118588..118953 (+) 366 WP_003055257.1 DUF1033 family protein -
  H7678_RS00725 (SDSE89_00960) comGA/cglA 119034..119975 (+) 942 WP_003062163.1 competence type IV pilus ATPase ComGA Machinery gene
  H7678_RS00730 (SDSE89_00970) comYB 119911..120942 (+) 1032 WP_050807598.1 competence type IV pilus assembly protein ComGB Machinery gene
  H7678_RS00735 (SDSE89_00980) comYC 120944..121270 (+) 327 WP_003055259.1 competence type IV pilus major pilin ComGC Machinery gene
  H7678_RS00740 comGD 121245..121673 (+) 429 WP_037589807.1 competence type IV pilus minor pilin ComGD -
  H7678_RS00745 (SDSE89_00990) comGE 121681..121926 (+) 246 WP_003049263.1 competence type IV pilus minor pilin ComGE -
  H7678_RS00750 (SDSE89_01000) comGF 121907..122347 (+) 441 WP_003061780.1 competence type IV pilus minor pilin ComGF -
  H7678_RS00755 (SDSE89_01010) comGG 122370..122699 (+) 330 WP_003062466.1 competence type IV pilus minor pilin ComGG -
  H7678_RS00760 (SDSE89_01020) comYH 122762..123715 (+) 954 WP_003060701.1 class I SAM-dependent methyltransferase Machinery gene
  H7678_RS00765 (SDSE89_01030) - 123774..124970 (+) 1197 WP_003058485.1 acetate kinase -

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35166.27 Da        Isoelectric Point: 7.2836

>NTDB_id=82976 H7678_RS00725 WP_003062163.1 119034..119975(+) (comGA/cglA) [Streptococcus dysgalactiae subsp. equisimilis strain 89]
MVQALARSILKEAEQIQAQDIYILPKEDQYELLIRVGDERRLVDVYRGDRMAHLISHFKFVAGMTVGEKRRCQVGSCDYD
IGGDTLLSLRLSSVGNYRGQESLVIRLLHHQQRSLHYWFDGLKTVARQIGGRGLYLFAGPVGSGKTTLMHQLIADYYQGA
QVISIEDPVEIKNHQALQLQVNDSIGMTYDNLIKLSLRHRPDILIIGEIRDSQTARAVIRASLTGVLVFSTVHAKSISGV
YARLLELGVTRAELDNCLALVAYQRLLNGGALIDSTQTEFEHYSPNQWNQKIDDLLAAGHLSPEQARFEKIIQ

Nucleotide


Download         Length: 942 bp        

>NTDB_id=82976 H7678_RS00725 WP_003062163.1 119034..119975(+) (comGA/cglA) [Streptococcus dysgalactiae subsp. equisimilis strain 89]
ATGGTACAAGCATTAGCAAGATCTATTTTGAAAGAAGCTGAGCAGATTCAAGCTCAAGATATCTATATTTTGCCAAAGGA
AGATCAGTATGAGCTATTGATAAGGGTAGGAGATGAAAGGAGATTGGTGGATGTTTATCGGGGCGATCGGATGGCTCATC
TTATTAGCCACTTTAAGTTCGTTGCAGGAATGACCGTTGGTGAGAAACGACGCTGTCAAGTCGGTTCTTGTGATTATGAT
ATCGGTGGAGACACACTTTTGTCTCTACGCCTGTCTAGTGTTGGAAATTACCGTGGACAAGAGAGCCTAGTGATTCGGTT
ATTACATCATCAACAAAGAAGTTTACATTACTGGTTTGACGGACTGAAAACAGTTGCACGTCAGATTGGAGGCCGAGGAC
TCTATCTTTTTGCAGGTCCAGTCGGATCAGGCAAAACGACTTTGATGCACCAATTGATTGCTGATTATTATCAAGGAGCA
CAGGTCATTAGCATAGAAGATCCGGTGGAAATTAAAAATCATCAAGCGCTCCAATTACAAGTTAATGATAGTATTGGTAT
GACTTACGACAACTTGATCAAACTATCTTTACGGCATCGCCCTGATATTTTAATCATTGGTGAAATTCGAGACAGCCAGA
CAGCTAGAGCGGTTATTAGAGCAAGTTTAACAGGTGTCTTAGTATTTTCAACAGTTCATGCCAAAAGCATTTCTGGTGTT
TATGCCAGATTACTAGAACTAGGAGTGACAAGAGCAGAATTAGACAATTGTTTGGCTTTGGTTGCTTACCAACGATTGCT
TAATGGAGGAGCATTGATTGACTCAACTCAAACAGAATTTGAACACTACTCACCAAACCAATGGAATCAGAAAATTGATG
ACCTTCTTGCAGCGGGACATCTCAGTCCGGAGCAAGCAAGGTTTGAAAAAATTATCCAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

61.935

99.042

0.613
  comYA Streptococcus mutans UA140

61.415

99.361

0.61
  comYA Streptococcus mutans UA159

61.415

99.361

0.61
  comYA Streptococcus gordonii str. Challis substr. CH1

61.093

99.361

0.607
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

60.129

99.361

0.597
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

59.807

99.361

0.594
  comGA/cglA/cilD Streptococcus pneumoniae D39

59.807

99.361

0.594
  comGA/cglA/cilD Streptococcus pneumoniae R6

59.807

99.361

0.594
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

59.807

99.361

0.594
  comGA Lactococcus lactis subsp. cremoris KW2

52.09

99.361

0.518
  comGA Latilactobacillus sakei subsp. sakei 23K

38.908

93.61

0.364

Multiple sequence alignment