Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   SH576_RS26575 Genome accession   NZ_CP139036
Coordinates   5599539..5600078 (-) Length   179 a.a.
NCBI ID   WP_088132826.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain MAS152     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5594539..5605078
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SH576_RS26555 (SH576_26535) - 5595224..5597479 (-) 2256 WP_033990664.1 DUF1631 domain-containing protein -
  SH576_RS26560 (SH576_26540) nadC 5597765..5598613 (+) 849 WP_033990663.1 carboxylating nicotinate-nucleotide diphosphorylase -
  SH576_RS26570 (SH576_26550) - 5598777..5599334 (-) 558 WP_224754157.1 hypothetical protein -
  SH576_RS26575 (SH576_26555) pilE 5599539..5600078 (-) 540 WP_088132826.1 pilin Machinery gene
  SH576_RS26580 (SH576_26560) pilB 5600307..5602010 (+) 1704 WP_003116249.1 type IV-A pilus assembly ATPase PilB Machinery gene
  SH576_RS26585 (SH576_26565) pilC 5602014..5603231 (+) 1218 WP_003116250.1 type II secretion system F family protein Machinery gene
  SH576_RS26590 (SH576_26570) pilD 5603234..5604106 (+) 873 WP_003116251.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  SH576_RS26595 (SH576_26575) coaE 5604103..5604714 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  SH576_RS26600 (SH576_26580) yacG 5604711..5604911 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 179 a.a.        Molecular weight: 18082.50 Da        Isoelectric Point: 7.7777

>NTDB_id=829055 SH576_RS26575 WP_088132826.1 5599539..5600078(-) (pilE) [Pseudomonas aeruginosa strain MAS152]
MKAQKGFTLIELMIVVAIIGILAAVALPAYQDYTIRARVTEGVGLAASAKTLIGDSSATAGELAASARVWNAQAGNAGAT
SKYVTSVRIAEATGEITVTFNATNVGNIPADSTLVFTPYVQNAAGAPTQLGASYAAGVTGSIDWGCASKSNAVSSGPDRN
MPALTAGTLPARFAPSECR

Nucleotide


Download         Length: 540 bp        

>NTDB_id=829055 SH576_RS26575 WP_088132826.1 5599539..5600078(-) (pilE) [Pseudomonas aeruginosa strain MAS152]
ATGAAAGCTCAGAAAGGCTTTACCTTGATCGAACTGATGATCGTGGTTGCGATCATCGGTATTCTTGCTGCCGTCGCTTT
GCCGGCATATCAGGATTACACCATTCGTGCTCGCGTGACAGAGGGGGTTGGTCTGGCTGCCAGCGCCAAGACGCTTATTG
GCGATAGCTCTGCCACTGCCGGTGAGCTAGCCGCTTCGGCAAGGGTCTGGAATGCTCAAGCCGGTAACGCCGGTGCTACC
AGTAAGTATGTGACCTCTGTACGAATTGCAGAGGCGACTGGTGAAATCACTGTTACTTTCAATGCCACAAACGTGGGTAA
TATTCCGGCTGACTCTACCCTGGTATTTACTCCCTATGTGCAGAATGCTGCCGGTGCCCCGACTCAATTGGGTGCCAGTT
ATGCTGCCGGTGTGACTGGCTCTATTGACTGGGGTTGTGCATCGAAATCCAATGCGGTTTCCAGTGGTCCCGACCGCAAT
ATGCCTGCCCTGACTGCAGGTACCCTGCCGGCTCGTTTCGCTCCGAGCGAATGCCGCTAA

Domains

Predicted by InterProScan.

Pilin (bacterial filament) (Pilin)

(36-179)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

42.64

100

0.469
  pilA Ralstonia pseudosolanacearum GMI1000

44.324

100

0.458
  pilA2 Legionella pneumophila str. Paris

41.808

98.883

0.413
  pilA2 Legionella pneumophila strain ERS1305867

41.243

98.883

0.408
  comP Acinetobacter baylyi ADP1

38.333

100

0.385
  pilE Neisseria gonorrhoeae strain FA1090

35.714

100

0.363