Detailed information NTG0082655

Bioinformatically predicted

Overview

• Name: comYF
• Type: Machinery gene
• Locus tag: KUN2590_RS00675
• Genome accession: NZ_AP023387
• Coordinates: 104886..105320 (+)
• Length: 144 a.a.
• NCBI ID: WP_002992738.1
• Uniprot ID: -
• Organism: Streptococcus pyogenes strain NIH34
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 99886..110320

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KUN2590_RS00645 (SPNIH34_00790)	-	101555..101920 (+)	366	WP_002986560.1	DUF1033 family protein	-
KUN2590_RS00650 (SPNIH34_00800)	comYA	102013..102951 (+)	939	WP_011054114.1	competence type IV pilus ATPase ComGA	Machinery gene
KUN2590_RS00655 (SPNIH34_00810)	comYB	102887..103921 (+)	1035	WP_011054115.1	competence type IV pilus assembly protein ComGB	Machinery gene
KUN2590_RS00660 (SPNIH34_00820)	comYC	103923..104249 (+)	327	WP_002986552.1	competence type IV pilus major pilin ComGC	Machinery gene
KUN2590_RS00665 (SPNIH34_00830)	comGD	104224..104652 (+)	429	WP_002986548.1	competence type IV pilus minor pilin ComGD	-
KUN2590_RS00670 (SPNIH34_00840)	comGE	104609..104893 (+)	285	WP_002987779.1	competence type IV pilus minor pilin ComGE	-
KUN2590_RS00675 (SPNIH34_00850)	comYF	104886..105320 (+)	435	WP_002992738.1	competence type IV pilus minor pilin ComGF	Machinery gene
KUN2590_RS00680 (SPNIH34_00860)	comGG	105304..105630 (+)	327	WP_010921801.1	competence type IV pilus minor pilin ComGG	-
KUN2590_RS00685 (SPNIH34_00870)	comYH	105728..106681 (+)	954	WP_011054117.1	class I SAM-dependent methyltransferase	Machinery gene
KUN2590_RS00690 (SPNIH34_00880)	-	106740..107936 (+)	1197	WP_010921803.1	acetate kinase	-
KUN2590_RS00695 (SPNIH34_00890)	-	108122..108430 (+)	309	WP_011054118.1	hypothetical protein	-
KUN2590_RS00700 (SPNIH34_00900)	proC	108513..109283 (-)	771	WP_002992745.1	pyrroline-5-carboxylate reductase	-

Sequence

Protein

• Length: 144 a.a.
• Molecular weight: 16645.27 Da
• Isoelectric Point: 10.2526

>NTDB_id=82655 KUN2590_RS00675 WP_002992738.1 104886..105320(+) (comYF) [Streptococcus pyogenes strain NIH34]
MSKQLSNIKAFTLLEALIALLVISGSLLVYQGLTQTLLKRSHYLARHDQDNWLLFSHQLREELSGARFYKVADNKLYVEK
GKKVLAFGQFKSHDFRKSASNGKGYQPMLFGISRSHIHIEQSQICITLKWKSGLERTFYYAFQD

Nucleotide

• Length: 435 bp

>NTDB_id=82655 KUN2590_RS00675 WP_002992738.1 104886..105320(+) (comYF) [Streptococcus pyogenes strain NIH34]
TTGAGTAAACAATTAAGTAATATAAAAGCTTTTACCCTTCTAGAGGCGTTAATAGCCTTACTCGTGATATCAGGGTCTTT
ATTGGTTTATCAAGGTTTGACCCAAACCCTCCTTAAACGTAGCCATTATCTAGCCCGTCATGATCAAGACAATTGGCTCT
TATTTTCTCATCAATTGCGAGAGGAGCTAAGTGGAGCAAGATTTTACAAAGTAGCTGATAATAAACTATACGTTGAAAAA
GGAAAGAAAGTACTAGCTTTTGGCCAATTTAAAAGTCATGATTTCCGAAAATCAGCTAGTAATGGAAAAGGGTATCAACC
CATGTTATTTGGAATATCACGTAGTCATATTCACATAGAGCAGTCACAGATTTGCATTACTTTAAAGTGGAAAAGTGGGT
TAGAAAGGACTTTTTATTATGCCTTTCAAGACTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (41-135)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comYF	Streptococcus mutans UA140	51.079	96.528	0.493
comYF	Streptococcus mutans UA159	50.36	96.528	0.486
comGF	Lactococcus lactis subsp. cremoris KW2	46.715	95.139	0.444
comGF/cglF	Streptococcus mitis NCTC 12261	44.776	93.056	0.417
comGF/cglF	Streptococcus pneumoniae Rx1	43.609	92.361	0.403
comGF/cglF	Streptococcus pneumoniae D39	43.609	92.361	0.403
comGF/cglF	Streptococcus pneumoniae R6	43.609	92.361	0.403
comGF/cglF	Streptococcus pneumoniae TIGR4	43.609	92.361	0.403
comGF/cglF	Streptococcus mitis SK321	42.857	92.361	0.396