Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   K9V56_RS05205 Genome accession   NZ_CP124551
Coordinates   1186800..1188467 (-) Length   555 a.a.
NCBI ID   WP_171827790.1    Uniprot ID   -
Organism   Kinneretia aquatilis strain DH15     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1181800..1193467
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K9V56_RS05175 (K9V56_005175) - 1182237..1182878 (+) 642 WP_171827659.1 HAD family hydrolase -
  K9V56_RS05180 (K9V56_005180) - 1182864..1183055 (-) 192 WP_171827658.1 DNA gyrase inhibitor YacG -
  K9V56_RS05185 (K9V56_005185) zapD 1183096..1183851 (-) 756 WP_102766589.1 cell division protein ZapD -
  K9V56_RS05190 (K9V56_005190) coaE 1183967..1184587 (-) 621 WP_263299991.1 dephospho-CoA kinase -
  K9V56_RS05195 (K9V56_005195) - 1184578..1185510 (-) 933 WP_171827657.1 prepilin peptidase -
  K9V56_RS05200 (K9V56_005200) pilC 1185523..1186743 (-) 1221 WP_171827656.1 type II secretion system F family protein Machinery gene
  K9V56_RS05205 (K9V56_005205) pilF 1186800..1188467 (-) 1668 WP_171827790.1 type IV-A pilus assembly ATPase PilB Machinery gene
  K9V56_RS05210 (K9V56_005210) - 1188890..1191106 (-) 2217 WP_285805973.1 ATP-binding protein -
  K9V56_RS05215 (K9V56_005215) - 1191216..1192085 (-) 870 WP_223696749.1 phosphate/phosphite/phosphonate ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 555 a.a.        Molecular weight: 60837.07 Da        Isoelectric Point: 6.0015

>NTDB_id=826430 K9V56_RS05205 WP_171827790.1 1186800..1188467(-) (pilF) [Kinneretia aquatilis strain DH15]
MLVHAGKLQPKAAEDLMRQAREKKTSFVNSVIAAGLVSPVDLAHTLSTALAVPLLDLNAMDVQRMPQNIIDNKLAQQYQV
VVLGRRGSRLFIGGADPTDQDVVERIKFATQLTPEWVIVEHDKLTKLLAGASSSASEALETITGGDFDFDIAEEESAPQE
TAAELADVEDAPVVRFLQKMLVDAINLRASDLHFEPYEFHYRVRFRVDGELREITQPPIAIKDKLASRIKVLSRLDIAER
RVPQDGRMKLKFGAKAIDFRISTLPTLFGEKIVIRILDPSSAKLGIDALGYEKIEKERLLAAISRPYGMVLVTGPTGSGK
TVSLYTCLNILNQPGVNISTVEDPAEINLPGINQVNVNDKAGLSFSAALKSFLRQDPDIIMVGEIRDLETADIAIKAAQT
GHMVMSTLHTNDAPKTLTRLLNMGVAPFNIASSVLLITAQRLVRRLCENCKAPAEYPREALLRAGFKEEELDGSWKQYRA
VGCNSCNSGYRGRVGLYQVMPITEAIQRIILAEGTAMDIAAQAQSEGVRDLRQFGLVKVRSGVTTLEEVLAATNE

Nucleotide


Download         Length: 1668 bp        

>NTDB_id=826430 K9V56_RS05205 WP_171827790.1 1186800..1188467(-) (pilF) [Kinneretia aquatilis strain DH15]
ATGCTGGTGCATGCCGGCAAGCTGCAGCCCAAAGCGGCCGAAGACCTGATGCGCCAGGCGCGCGAGAAGAAGACCAGCTT
CGTCAATTCGGTGATCGCGGCGGGGCTGGTGAGTCCGGTGGATCTGGCCCACACCCTGTCCACAGCCCTGGCCGTGCCGC
TGCTGGACCTGAATGCCATGGATGTTCAGCGCATGCCGCAGAACATCATCGACAACAAGCTGGCCCAGCAGTACCAGGTG
GTGGTGCTGGGGCGGCGCGGCAGCCGGCTCTTCATCGGCGGCGCCGACCCGACTGACCAGGACGTGGTCGAGCGCATCAA
GTTCGCCACCCAGCTGACGCCGGAATGGGTGATCGTCGAGCATGACAAGCTGACCAAGCTGCTGGCGGGCGCCAGCTCCA
GTGCCAGCGAGGCCCTGGAAACCATCACTGGCGGTGATTTCGATTTCGACATTGCCGAAGAAGAAAGCGCGCCGCAGGAG
ACTGCCGCCGAACTGGCCGATGTGGAAGACGCACCCGTGGTGCGTTTTCTCCAGAAGATGCTGGTCGACGCCATCAATCT
GCGCGCGTCCGACCTGCATTTCGAGCCCTACGAGTTCCATTACCGCGTGCGCTTTCGCGTGGACGGCGAGCTGCGCGAAA
TCACCCAGCCGCCGATCGCGATCAAGGACAAGCTGGCTTCGCGCATCAAGGTGCTGTCGCGCCTGGACATTGCCGAACGT
CGCGTGCCGCAAGACGGCCGCATGAAGCTGAAGTTCGGCGCCAAGGCCATCGACTTCCGGATCAGCACCTTGCCGACCCT
GTTCGGCGAGAAGATCGTGATCCGTATCCTGGACCCGTCCTCGGCCAAGCTGGGCATCGACGCCCTGGGTTACGAAAAGA
TCGAGAAAGAGCGCCTGCTGGCCGCCATCTCCCGCCCCTACGGCATGGTGCTGGTGACCGGCCCGACCGGCTCGGGCAAG
ACCGTGTCGCTCTACACCTGCCTGAACATCCTGAACCAGCCGGGCGTCAACATCTCGACCGTGGAAGACCCGGCCGAAAT
CAACCTGCCCGGCATCAACCAGGTCAATGTCAATGACAAGGCGGGCCTGAGCTTCTCGGCCGCGCTGAAGTCTTTCCTGC
GTCAGGATCCCGACATCATCATGGTCGGCGAAATCCGCGACCTGGAAACCGCCGACATCGCCATCAAGGCCGCCCAGACC
GGCCATATGGTGATGTCCACCCTGCACACCAACGACGCGCCCAAGACCCTGACCCGGCTCTTGAACATGGGCGTGGCCCC
GTTCAACATCGCGTCCAGCGTGCTGCTCATCACGGCCCAGCGCCTGGTGCGGCGCCTGTGCGAAAACTGCAAGGCCCCGG
CCGAATACCCGCGCGAAGCCCTGCTGCGTGCCGGCTTCAAGGAAGAAGAACTGGACGGCAGCTGGAAACAGTACCGCGCC
GTGGGCTGCAACAGCTGCAACAGCGGCTACCGCGGCCGGGTCGGCCTCTACCAGGTGATGCCCATCACCGAGGCCATCCA
GCGCATCATCCTGGCCGAAGGCACGGCCATGGACATCGCGGCCCAGGCCCAGTCCGAAGGGGTGCGCGATCTGCGTCAGT
TCGGCCTGGTCAAGGTCCGCTCGGGCGTGACCACGCTCGAAGAAGTGCTGGCGGCGACCAATGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(179-445)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(32-133)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

56.144

95.315

0.535
  pilB Acinetobacter baumannii D1279779

54.647

96.937

0.53
  pilB Acinetobacter baylyi ADP1

52.158

100

0.523
  pilB Legionella pneumophila strain ERS1305867

51.075

100

0.514
  pilB Vibrio parahaemolyticus RIMD 2210633

44.425

100

0.452
  pilB Vibrio cholerae strain A1552

45.704

98.559

0.45
  pilB Vibrio campbellii strain DS40M4

44.964

100

0.45
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.587

96.036

0.38
  pilF Thermus thermophilus HB27

37.367

100

0.378