Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   QHG62_RS13225 Genome accession   NZ_CP123990
Coordinates   2722785..2724524 (-) Length   579 a.a.
NCBI ID   WP_281151286.1    Uniprot ID   -
Organism   Variovorax paradoxus strain MGMM5     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2717785..2729524
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QHG62_RS13205 (QHG62_13205) - 2718376..2719821 (-) 1446 WP_281151283.1 MBOAT family protein -
  QHG62_RS13210 (QHG62_13210) coaE 2719945..2720550 (-) 606 WP_281151618.1 dephospho-CoA kinase -
  QHG62_RS13215 (QHG62_13215) - 2720561..2721526 (-) 966 WP_281151284.1 A24 family peptidase -
  QHG62_RS13220 (QHG62_13220) pilC 2721526..2722755 (-) 1230 WP_281151285.1 type II secretion system F family protein Machinery gene
  QHG62_RS13225 (QHG62_13225) pilB 2722785..2724524 (-) 1740 WP_281151286.1 type IV-A pilus assembly ATPase PilB Machinery gene
  QHG62_RS13235 (QHG62_13235) - 2724766..2725758 (-) 993 WP_281151620.1 polyprenyl synthetase family protein -
  QHG62_RS13240 (QHG62_13240) rplU 2725950..2726261 (+) 312 WP_028259151.1 50S ribosomal protein L21 -
  QHG62_RS13245 (QHG62_13245) rpmA 2726274..2726534 (+) 261 WP_028259150.1 50S ribosomal protein L27 -
  QHG62_RS13250 (QHG62_13250) cgtA 2726605..2727681 (+) 1077 WP_281151288.1 Obg family GTPase CgtA -
  QHG62_RS13255 (QHG62_13255) proB 2727796..2728935 (+) 1140 WP_281151289.1 glutamate 5-kinase -

Sequence

Protein


Download         Length: 579 a.a.        Molecular weight: 62840.01 Da        Isoelectric Point: 5.7633

>NTDB_id=823619 QHG62_RS13225 WP_281151286.1 2722785..2724524(-) (pilB) [Variovorax paradoxus strain MGMM5]
MAAAELPVKENTQIALPGLARALVSAGKLPAKTAEDIYQKSLSSRTSFIAELTGSGAVSAADLAHTLSSAFGAPLLDLDA
IDHQRLPKDLLDPKLCLAYRIVVLSKRNNRLIVATADPSDQQAVEKIKFASQMGVDWVIAEYDKLSRMIEAAAVSASETL
NNIVGGGDFEFDDVTADTSGDTNEQAAIAEVEDAPVVRFLHKMLLDGVSMRASDIHFEPYEHNYRVRFRIDGELREIASP
PTLIKDKLASRIKVISRLDISEKRIPQDGRMKLKIGPDRVIDFRVSTLPTLFGEKIVIRILDPSSARLGIDALGYDADEK
ERLLNAIGRPYGMVLVTGPTGSGKTVSLYTCLNLLNQPGVNIATAEDPSEINLPGVNQVNVNERAGLTFAAALRAFLRQD
PDIIMVGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCHVCRAPADV
PRQTLLDAGFKEEELDGSWKPYRPVGCSACNSGYKGRVGIYQVMPISEAIQAIILRDGSALDIARQSEAEGVRSLRQSGL
RKVMQGLTSLEEVVAVTNE

Nucleotide


Download         Length: 1740 bp        

>NTDB_id=823619 QHG62_RS13225 WP_281151286.1 2722785..2724524(-) (pilB) [Variovorax paradoxus strain MGMM5]
ATGGCTGCCGCCGAACTTCCTGTTAAAGAAAACACGCAAATCGCCCTGCCAGGCCTTGCGCGCGCCCTCGTCTCGGCCGG
CAAGCTGCCCGCGAAAACTGCGGAAGACATCTATCAGAAGTCGCTGAGCAGCCGCACCAGCTTCATTGCGGAGCTGACCG
GCAGCGGCGCCGTCTCGGCGGCGGACCTGGCCCATACGCTTTCTTCGGCCTTTGGCGCCCCGCTGCTCGATCTGGACGCA
ATCGATCACCAGCGCCTGCCCAAGGACCTGCTCGACCCCAAGCTGTGCCTTGCGTACCGCATCGTCGTGCTCAGCAAGCG
CAACAACCGTCTTATTGTTGCAACAGCCGACCCCTCGGACCAGCAGGCGGTGGAAAAGATCAAGTTCGCATCCCAGATGG
GCGTGGACTGGGTCATTGCCGAGTACGACAAGCTGTCGCGCATGATCGAGGCGGCCGCGGTCAGCGCGTCGGAAACCCTC
AACAATATCGTCGGCGGCGGGGATTTCGAGTTCGACGACGTCACGGCGGACACCTCGGGCGATACGAACGAACAGGCGGC
CATCGCCGAAGTCGAGGATGCCCCGGTCGTTCGCTTCCTTCACAAGATGCTGCTTGACGGCGTCAGCATGCGCGCATCGG
ACATTCACTTCGAGCCGTACGAGCACAACTATCGGGTGCGCTTTCGCATCGACGGCGAACTGCGCGAAATTGCCAGCCCG
CCCACGCTCATCAAGGACAAGCTGGCCTCGCGCATCAAGGTTATCTCCCGGCTCGACATCTCCGAAAAGCGCATTCCGCA
AGACGGGCGGATGAAGCTCAAGATCGGCCCGGACCGCGTGATCGACTTTCGCGTGAGCACCCTGCCCACGCTGTTCGGCG
AGAAGATCGTGATCCGTATTCTGGATCCGAGCAGCGCGCGCCTGGGCATCGATGCGCTCGGTTACGACGCGGATGAAAAG
GAACGGCTGCTGAACGCGATCGGCCGCCCCTACGGCATGGTGCTGGTAACGGGCCCCACTGGTTCGGGCAAGACGGTTTC
GCTCTATACCTGCCTGAACTTGCTGAACCAGCCCGGCGTCAACATTGCCACGGCGGAAGACCCGTCCGAAATCAACCTGC
CCGGGGTGAACCAGGTCAACGTCAACGAACGTGCCGGACTCACGTTCGCCGCCGCGCTGCGTGCCTTCCTCCGGCAGGAT
CCCGACATCATCATGGTCGGCGAAATCCGCGACCTCGAAACCGCAGACATCTCCATCAAGGCCGCGCAAACGGGCCACCT
GGTGCTGTCGACGCTACACACCAACGATGCGCCGACCACGCTCACGCGCATGCGGAACATGGGCATCGCGCCGTTCAACA
TCGCCTCGAGCGTCATCCTGATCACTGCGCAGCGGCTGGCCCGCCGCCTGTGCCACGTCTGCCGTGCGCCGGCAGATGTG
CCCCGCCAGACCCTGCTCGATGCAGGCTTCAAGGAAGAAGAGCTCGACGGCTCCTGGAAGCCCTATCGACCGGTCGGCTG
CTCCGCGTGCAATAGCGGCTACAAGGGCCGCGTCGGCATCTACCAGGTGATGCCGATCTCCGAAGCGATCCAGGCCATCA
TCCTGCGCGACGGCAGCGCGTTGGACATTGCGCGCCAGTCCGAGGCCGAGGGCGTCCGCTCGCTGCGGCAGTCGGGCCTC
AGAAAAGTCATGCAAGGCCTCACCTCACTCGAGGAAGTGGTGGCGGTCACCAACGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(202-469)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(54-153)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

54.021

98.791

0.534
  pilB Acinetobacter baylyi ADP1

53.076

98.273

0.522
  pilF Neisseria gonorrhoeae MS11

52.035

97.582

0.508
  pilB Legionella pneumophila strain ERS1305867

49.735

97.582

0.485
  pilB Vibrio cholerae strain A1552

45.899

98.964

0.454
  pilB Vibrio parahaemolyticus RIMD 2210633

45.614

98.446

0.449
  pilB Vibrio campbellii strain DS40M4

45.053

97.755

0.44
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.226

91.883

0.37
  pilF Thermus thermophilus HB27

37.368

98.446

0.368