Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   R6K63_RS10420 Genome accession   NZ_CP137556
Coordinates   2173687..2175195 (+) Length   502 a.a.
NCBI ID   WP_003091377.1    Uniprot ID   A0A0H2ZC25
Organism   Pseudomonas aeruginosa strain CDC1270     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2168687..2180195
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R6K63_RS10400 - 2168712..2169923 (+) 1212 WP_003113933.1 O-succinylhomoserine sulfhydrylase -
  R6K63_RS10405 - 2169920..2170687 (+) 768 WP_023108530.1 SDR family oxidoreductase -
  R6K63_RS10410 xcpQ 2170782..2172755 (-) 1974 WP_023436424.1 GspD family T2SS secretin variant XcpQ -
  R6K63_RS10415 xcpP 2172760..2173467 (-) 708 WP_042173362.1 type II secretion system protein N -
  R6K63_RS10420 pilB 2173687..2175195 (+) 1509 WP_003091377.1 GspE family T2SS ATPase variant XcpR Machinery gene
  R6K63_RS10425 xcpS 2175195..2176412 (+) 1218 WP_003091376.1 GspF family T2SS innner membrane protein variant XcpS -
  R6K63_RS10430 xcpT 2176417..2176863 (+) 447 WP_003130409.1 GspG family T2SS major pseudopilin variant XcpT -
  R6K63_RS10435 xcpU 2176870..2177388 (+) 519 WP_003110066.1 GspH family T2SS minor pseudopilin variant XcpU -
  R6K63_RS10440 xcpV 2177385..2177774 (+) 390 WP_023108527.1 GspI family T2SS minor pseudopilin variant XcpV -
  R6K63_RS10445 xcpW 2177771..2178484 (+) 714 WP_003110067.1 GspJ family T2SS minor pseudopilin variant XcpW -
  R6K63_RS10450 xcpX 2178481..2179482 (+) 1002 WP_023436422.1 GspK family T2SS minor pseudopilin variant XcpX -

Sequence

Protein


Download         Length: 502 a.a.        Molecular weight: 55524.73 Da        Isoelectric Point: 6.2074

>NTDB_id=822341 R6K63_RS10420 WP_003091377.1 2173687..2175195(+) (pilB) [Pseudomonas aeruginosa strain CDC1270]
MMTAPLPDIAAPAAPPRRLPFSFAKRQGLLFLCLEEQYWLACRPQVELAAIAEAQRFAGRRLPLKALGEDAFNQALAASY
QHDSSAAMQLAEDLGGSLDLAALADQVPETEDLMEQEDDAPIIRLINAILGEAIRENASDIHLETFEKRLVVRFRVDGVL
REVLEPKRELAALLVSRIKVMARLDIAEKRIPQDGRISLRVGGREVDIRVSTLPSANGERVVLRLLDKQAGRLNLQHLGM
SERDRKLMDETVRKPHGILLVTGPTGSGKTTTLYASLTTLNDRTRNILTVEDPIEYHLEGIGQTQVNAKVDMTFARGLRA
ILRQDPDVVMVGEIRDRETAEIAVQASLTGHLVLSTLHTNSAIGAITRLVDMGIEPFLLSSSMLGVLAQRLVRVLCPACK
EPYRADEAECALLGVDPAAPPTLHRARGCGECHQHGYRGRTGIYELVVFDDHMRSLIHNESSEQEMTRHARTSGPSIRDD
GRRKVLEGVTTVEEVLRVTREE

Nucleotide


Download         Length: 1509 bp        

>NTDB_id=822341 R6K63_RS10420 WP_003091377.1 2173687..2175195(+) (pilB) [Pseudomonas aeruginosa strain CDC1270]
ATGATGACAGCCCCGTTACCCGACATCGCCGCGCCGGCCGCACCGCCACGGCGTCTGCCGTTCAGTTTCGCCAAGCGCCA
GGGTCTGCTGTTCCTGTGCCTGGAGGAGCAGTACTGGCTGGCCTGCCGGCCGCAGGTGGAACTGGCGGCGATCGCCGAGG
CGCAGCGCTTCGCCGGCCGCCGCCTGCCGCTCAAGGCGCTCGGCGAGGATGCCTTCAACCAGGCCCTGGCGGCGTCCTAC
CAGCACGATTCCTCGGCGGCCATGCAGCTCGCCGAGGACCTCGGCGGCAGCCTGGACCTCGCCGCCCTGGCCGACCAGGT
CCCGGAAACCGAGGACCTGATGGAGCAGGAGGACGACGCGCCGATCATTCGCCTGATCAACGCGATCCTCGGCGAGGCGA
TCCGCGAGAACGCTTCGGACATCCACCTGGAAACCTTCGAGAAACGCCTGGTGGTGCGCTTCCGCGTCGACGGCGTGCTG
CGCGAGGTGCTCGAACCCAAGCGCGAGCTGGCGGCCTTGCTGGTATCGCGGATCAAGGTCATGGCGCGGCTGGACATCGC
CGAGAAACGCATTCCCCAGGATGGACGGATTTCCCTGCGGGTCGGCGGGCGTGAGGTGGACATCCGCGTCTCGACCCTGC
CCTCGGCCAACGGCGAGCGCGTGGTGCTGCGTCTGCTCGACAAGCAGGCCGGGCGCCTGAACCTGCAGCACCTGGGCATG
AGCGAGCGCGACCGCAAGCTGATGGACGAAACCGTGCGCAAGCCGCACGGCATCCTGCTGGTCACCGGGCCCACCGGTTC
GGGCAAGACCACCACCCTGTACGCCAGCCTGACCACGCTCAACGACCGCACGCGGAACATCCTCACCGTCGAGGATCCGA
TCGAATACCACCTGGAAGGCATCGGCCAGACCCAGGTCAACGCCAAGGTCGACATGACCTTCGCCCGCGGCCTGCGCGCG
ATCCTCCGGCAGGACCCGGATGTGGTGATGGTCGGCGAGATCCGTGACCGGGAGACTGCCGAGATCGCGGTGCAGGCTTC
GCTGACCGGCCACCTGGTGCTCTCGACCCTGCACACCAACAGCGCCATCGGCGCGATCACCCGCCTGGTGGACATGGGCA
TCGAGCCGTTCCTGCTGTCCTCCTCCATGCTTGGCGTGCTGGCCCAGCGCCTGGTGCGGGTGCTCTGCCCGGCGTGCAAG
GAGCCCTACCGGGCCGACGAGGCCGAGTGCGCGCTGCTCGGCGTCGATCCCGCCGCGCCGCCGACCCTGCATCGCGCCCG
CGGCTGCGGCGAGTGCCACCAGCATGGCTATCGCGGGCGTACCGGTATCTATGAACTGGTGGTGTTCGACGACCACATGC
GCTCGCTGATCCACAACGAATCCTCCGAGCAGGAAATGACCCGGCATGCCCGCACCAGTGGACCGAGCATCCGCGACGAC
GGTCGGCGCAAGGTGCTGGAAGGCGTCACCACGGTCGAGGAAGTCCTGCGCGTGACCCGGGAAGAATAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(128-394)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2ZC25

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

43.532

97.012

0.422
  pilB/pilB1 Synechocystis sp. PCC 6803

42.245

97.61

0.412
  pilB Vibrio campbellii strain DS40M4

43.249

94.422

0.408
  pilB Vibrio parahaemolyticus RIMD 2210633

47.045

84.263

0.396
  pilF Thermus thermophilus HB27

43.207

89.442

0.386
  pilB Acinetobacter baumannii D1279779

47.88

79.88

0.382
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.446

76.892

0.373
  pilF Neisseria gonorrhoeae MS11

46.954

78.486

0.369
  pilB Acinetobacter baylyi ADP1

47.68

77.291

0.369
  pilB Legionella pneumophila strain ERS1305867

47.423

77.291

0.367