Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   QC826_RS23990 Genome accession   NZ_CP122407
Coordinates   5211855..5213579 (+) Length   574 a.a.
NCBI ID   WP_279762765.1    Uniprot ID   -
Organism   Rugamonas sp. DEMB1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5206855..5218579
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QC826_RS23970 (QC826_23970) guaD 5207464..5208774 (-) 1311 WP_279762763.1 guanine deaminase -
  QC826_RS23975 (QC826_23975) puuE 5208784..5209737 (-) 954 WP_279762764.1 allantoinase PuuE -
  QC826_RS23980 (QC826_23980) uraH 5209861..5210211 (+) 351 WP_099415112.1 hydroxyisourate hydrolase -
  QC826_RS23985 (QC826_23985) - 5210404..5211689 (+) 1286 Protein_4748 HlyC/CorC family transporter -
  QC826_RS23990 (QC826_23990) pilB 5211855..5213579 (+) 1725 WP_279762765.1 type IV-A pilus assembly ATPase PilB Machinery gene
  QC826_RS23995 (QC826_23995) pilC 5213590..5214807 (+) 1218 WP_279762767.1 type II secretion system F family protein Machinery gene
  QC826_RS24000 (QC826_24000) - 5214971..5215246 (+) 276 WP_279762769.1 hypothetical protein -
  QC826_RS24005 (QC826_24005) - 5215434..5217581 (+) 2148 WP_279762770.1 serine/threonine-protein kinase -
  QC826_RS24010 (QC826_24010) - 5217635..5218162 (+) 528 WP_279762771.1 hypothetical protein -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 62005.66 Da        Isoelectric Point: 6.1323

>NTDB_id=815477 QC826_RS23990 WP_279762765.1 5211855..5213579(+) (pilB) [Rugamonas sp. DEMB1]
MAAVIPNATPSTALPGLARALLQAGRLSAPQADALHKKAGNDKQAFIDVLLASGAIDARALALFCSETFAYPLLDLHSVN
LEALPPKVIDHRLMQSQRVVPLSKRGNRMSVAISDPTNIQALDQIKFQTESSVEPVIVAHDALLNLLAALNKNTEQSIND
MIGDEQDIQFAEEDSAAAPDPGANEVEDAPIVRFLNKMLMDAVNMGASDLHFEPFEKFYRIRFRVDGILMEHAQPPISIK
DKLVSRIKVLAKLDISEKRVPQDGRMRLIVSPTKTIDLRISTLPTLFGEKTVMRILDATQAQMGIDALGYDPDQKELLLD
AIQRPYGMVLVTGPTGSGKTVSLYTCLNVINKPGINISTAENPAEINLPGVNQVNVNDKAGLTFPVALKSFLRQAPDIIM
VGEIRDLETADIAIKAAQTGHMVFSTLHTNDAPSTLTRLMNMGVAPFNIASSVILITAQRLARRLCSCKQPVEIGDALLL
KAGFRADELDGSWKPYGPVGCERCNGGGYKGRVGIYQIMPITPAIEALILANGNAMQIAAQSESEGVKSLRKSGLVKVRN
GLTSLEEVLGCTNE

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=815477 QC826_RS23990 WP_279762765.1 5211855..5213579(+) (pilB) [Rugamonas sp. DEMB1]
ATGGCAGCAGTCATTCCCAACGCCACGCCCAGCACCGCCCTGCCGGGGCTGGCGCGGGCCTTGCTCCAGGCCGGGCGCCT
CAGCGCGCCCCAGGCCGACGCGCTGCACAAAAAAGCCGGCAACGATAAGCAGGCCTTCATCGACGTGCTGCTGGCCAGCG
GCGCGATCGACGCGCGCGCGTTGGCGCTGTTCTGCTCGGAAACCTTCGCCTACCCCCTGCTCGACCTGCACAGCGTCAAC
CTGGAGGCGCTGCCGCCCAAGGTGATCGACCACCGCCTGATGCAGAGCCAGCGCGTGGTGCCGCTGTCCAAGCGCGGCAA
CAGGATGTCGGTGGCCATCTCCGACCCCACCAACATCCAGGCGCTGGACCAGATCAAGTTCCAGACCGAGTCCTCGGTCG
AGCCGGTGATCGTCGCCCACGACGCCCTGTTGAACCTGCTGGCGGCGCTCAACAAGAACACCGAGCAAAGCATCAACGAC
ATGATCGGCGACGAGCAGGACATCCAGTTCGCCGAGGAGGACAGCGCCGCCGCGCCCGACCCCGGCGCCAACGAGGTGGA
GGACGCGCCCATCGTGCGCTTCCTCAACAAGATGCTGATGGACGCCGTCAACATGGGCGCCTCCGACCTGCACTTCGAAC
CGTTCGAGAAGTTCTACCGCATCCGCTTCCGCGTCGACGGCATATTGATGGAGCACGCCCAGCCGCCGATTTCGATCAAG
GACAAGCTGGTCTCGCGCATCAAGGTGCTGGCCAAGCTCGACATCTCGGAAAAGCGCGTGCCGCAGGACGGCCGCATGCG
CCTGATCGTCTCGCCGACCAAGACCATCGACCTGCGCATCAGCACCCTGCCCACCCTGTTCGGCGAGAAGACGGTGATGC
GCATCCTCGACGCCACCCAGGCGCAGATGGGCATCGACGCGCTCGGCTACGACCCGGACCAGAAGGAGCTGCTGCTCGAC
GCCATCCAGCGCCCCTACGGCATGGTGCTGGTGACCGGGCCGACCGGCTCGGGCAAGACGGTGTCGCTGTACACCTGCCT
GAACGTCATCAACAAGCCGGGCATCAACATCTCCACGGCCGAGAACCCGGCCGAGATCAACCTGCCCGGCGTCAACCAGG
TCAACGTCAACGACAAGGCCGGCCTGACCTTCCCGGTGGCGCTCAAGTCCTTCCTGCGGCAGGCCCCGGACATCATCATG
GTCGGCGAGATCCGCGACCTGGAGACGGCCGACATCGCCATCAAGGCGGCGCAGACCGGCCACATGGTGTTCTCCACCCT
GCACACCAACGACGCGCCGTCGACCCTGACGCGGCTGATGAACATGGGCGTGGCGCCGTTCAACATCGCCTCGTCGGTGA
TTTTGATCACCGCGCAGCGGCTGGCGCGGCGCCTGTGCAGCTGCAAGCAGCCGGTCGAGATCGGCGACGCGCTGCTGCTC
AAGGCCGGTTTCCGCGCCGACGAGCTCGACGGCAGCTGGAAGCCCTACGGCCCGGTCGGCTGCGAGCGCTGCAACGGCGG
CGGCTACAAGGGCCGGGTCGGCATCTACCAGATCATGCCGATCACGCCGGCCATCGAGGCGCTGATCCTGGCCAACGGCA
ACGCCATGCAGATCGCCGCCCAGTCCGAGAGCGAGGGCGTGAAATCGCTGCGCAAGTCCGGCCTGGTGAAGGTGAGGAAC
GGCTTGACCAGCCTGGAGGAAGTGCTGGGCTGCACCAACGAATAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(50-154)

Type II/IV secretion system protein (T2SSE)

(197-464)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

52.622

99.652

0.524
  pilF Neisseria gonorrhoeae MS11

52.113

98.955

0.516
  pilB Acinetobacter baylyi ADP1

52.669

97.909

0.516
  pilB Legionella pneumophila strain ERS1305867

46.737

98.78

0.462
  pilB Vibrio cholerae strain A1552

45.73

97.909

0.448
  pilB Vibrio parahaemolyticus RIMD 2210633

44.207

97.735

0.432
  pilB Vibrio campbellii strain DS40M4

43.617

98.258

0.429
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.943

98.258

0.373
  pilF Thermus thermophilus HB27

39.212

92.857

0.364