Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   QBS03_RS17945 Genome accession   NZ_CP121846
Coordinates   3851333..3852442 (-) Length   369 a.a.
NCBI ID   WP_279487311.1    Uniprot ID   -
Organism   Aeromonas veronii strain ANYA 15110     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3846333..3857442
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QBS03_RS17915 - 3846796..3847803 (-) 1008 WP_021230948.1 Fe(3+) ABC transporter substrate-binding protein -
  QBS03_RS17920 glnK 3848062..3848400 (-) 339 WP_005341406.1 P-II family nitrogen regulator -
  QBS03_RS17925 arfB 3848602..3849018 (+) 417 WP_279486142.1 alternative ribosome rescue aminoacyl-tRNA hydrolase ArfB -
  QBS03_RS17930 - 3849040..3849532 (+) 493 Protein_3453 methylated-DNA--[protein]-cysteine S-methyltransferase -
  QBS03_RS17935 - 3849892..3850317 (-) 426 WP_005340360.1 hypothetical protein -
  QBS03_RS17940 yaaA 3850512..3851285 (-) 774 WP_100646812.1 peroxide stress protein YaaA -
  QBS03_RS17945 pilU 3851333..3852442 (-) 1110 WP_279487311.1 type IVa pilus ATPase TapU Machinery gene
  QBS03_RS17950 pilT 3852471..3853505 (-) 1035 WP_005340354.1 type IVa pilus ATPase TapT Machinery gene
  QBS03_RS17955 - 3853545..3854246 (+) 702 WP_019445603.1 YggS family pyridoxal phosphate-dependent enzyme -
  QBS03_RS17960 proC 3854389..3855213 (+) 825 WP_279486143.1 pyrroline-5-carboxylate reductase -
  QBS03_RS17965 - 3855225..3855776 (+) 552 WP_047436998.1 YggT family protein -
  QBS03_RS17970 yggU 3855776..3856078 (+) 303 WP_201958626.1 DUF167 family protein YggU -
  QBS03_RS17975 - 3856122..3856541 (+) 420 WP_279486144.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41048.06 Da        Isoelectric Point: 6.3713

>NTDB_id=814696 QBS03_RS17945 WP_279487311.1 3851333..3852442(-) (pilU) [Aeromonas veronii strain ANYA 15110]
MNLDDLLSELVERKGSDLFVTVGSPPTLKVNGHLVSLGGEALDKKGALTLVRDTLSSDHFERYIRTKEANYAIYREALGR
FRVSAFWQQELPGMVVRRIETRIPTFEDLQLPKILQEVAMAKRGLVLFVGATGAGKSTTQAAMIGYRNQHADGHILTVED
PVEFVHQHGRSLVTQREVGIDTESFDVALKSSLRQAPDVILIGEIRSQETMEFALQFAETGHLCLATLHANNANQALDRI
LHLVPQDKHRQFLFDLSFNLKAIVAQQLVPSMDGKRRCAAFEILLNTPLITDIIRKGEMHRLKEVMTKSTELGMQTFDQA
LFSLFCAGQIGYSEALAHADSANDLRLLIKLSGREQLGSGTLENVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=814696 QBS03_RS17945 WP_279487311.1 3851333..3852442(-) (pilU) [Aeromonas veronii strain ANYA 15110]
ATGAATCTGGATGATCTGCTGAGCGAGCTGGTCGAGCGAAAGGGATCGGATCTGTTTGTGACGGTGGGCTCGCCGCCCAC
CCTCAAGGTGAATGGCCATCTGGTGTCGCTGGGGGGGGAGGCGCTCGATAAGAAGGGGGCGCTGACGCTGGTCAGGGATA
CCCTCAGTAGCGATCACTTCGAGCGCTATATCCGCACCAAGGAGGCCAACTACGCGATCTATCGCGAGGCTCTTGGTCGT
TTTCGGGTCAGCGCCTTCTGGCAGCAGGAGCTGCCCGGCATGGTGGTGCGGCGTATCGAGACCCGCATTCCTACCTTTGA
AGATCTGCAGCTACCCAAGATCCTGCAAGAGGTGGCGATGGCCAAGCGGGGGCTGGTGCTGTTTGTCGGCGCCACCGGAG
CGGGCAAATCGACTACCCAGGCGGCGATGATCGGCTATCGCAACCAGCATGCCGATGGTCATATTCTGACGGTGGAAGAT
CCGGTGGAGTTCGTCCATCAGCATGGTCGTAGTCTGGTGACCCAGCGAGAGGTGGGGATCGACACCGAGTCGTTCGATGT
GGCGCTAAAAAGCTCACTGCGTCAGGCGCCGGACGTGATCCTGATCGGCGAAATCCGAAGTCAGGAGACCATGGAGTTTG
CCCTGCAGTTCGCCGAGACGGGACACCTTTGTCTCGCCACTTTGCATGCCAACAATGCCAACCAGGCGCTGGATCGCATC
CTCCATCTGGTGCCGCAAGACAAACACCGCCAGTTCCTGTTCGATCTCTCTTTCAACCTCAAGGCCATCGTCGCCCAGCA
GCTGGTGCCGAGTATGGATGGCAAGCGGCGGTGCGCCGCGTTCGAGATCCTGCTCAATACCCCGCTCATCACTGACATTA
TCCGCAAGGGAGAGATGCATCGCCTCAAGGAGGTGATGACCAAATCCACCGAGCTGGGTATGCAGACCTTCGATCAAGCG
CTCTTCAGCCTGTTCTGCGCCGGCCAGATTGGCTACAGTGAGGCACTCGCCCACGCCGACTCGGCCAACGACCTGCGACT
GCTGATCAAGCTCTCCGGTCGCGAGCAGCTGGGGTCCGGCACACTGGAAAATGTGACGCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.598

99.729

0.604
  pilU Pseudomonas stutzeri DSM 10701

60.399

95.122

0.575
  pilU Acinetobacter baylyi ADP1

57.224

95.664

0.547
  pilT Legionella pneumophila strain Lp02

40.816

92.954

0.379
  pilT Legionella pneumophila strain ERS1305867

40.816

92.954

0.379
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.823

91.87

0.366
  pilT Acinetobacter baylyi ADP1

39.296

92.412

0.363
  pilT Vibrio cholerae strain A1552

39.82

90.515

0.36
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

39.82

90.515

0.36