Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   QBS03_RS17950 Genome accession   NZ_CP121846
Coordinates   3852471..3853505 (-) Length   344 a.a.
NCBI ID   WP_005340354.1    Uniprot ID   -
Organism   Aeromonas veronii strain ANYA 15110     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3847471..3858505
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QBS03_RS17920 glnK 3848062..3848400 (-) 339 WP_005341406.1 P-II family nitrogen regulator -
  QBS03_RS17925 arfB 3848602..3849018 (+) 417 WP_279486142.1 alternative ribosome rescue aminoacyl-tRNA hydrolase ArfB -
  QBS03_RS17930 - 3849040..3849532 (+) 493 Protein_3453 methylated-DNA--[protein]-cysteine S-methyltransferase -
  QBS03_RS17935 - 3849892..3850317 (-) 426 WP_005340360.1 hypothetical protein -
  QBS03_RS17940 yaaA 3850512..3851285 (-) 774 WP_100646812.1 peroxide stress protein YaaA -
  QBS03_RS17945 pilU 3851333..3852442 (-) 1110 WP_279487311.1 type IVa pilus ATPase TapU Machinery gene
  QBS03_RS17950 pilT 3852471..3853505 (-) 1035 WP_005340354.1 type IVa pilus ATPase TapT Machinery gene
  QBS03_RS17955 - 3853545..3854246 (+) 702 WP_019445603.1 YggS family pyridoxal phosphate-dependent enzyme -
  QBS03_RS17960 proC 3854389..3855213 (+) 825 WP_279486143.1 pyrroline-5-carboxylate reductase -
  QBS03_RS17965 - 3855225..3855776 (+) 552 WP_047436998.1 YggT family protein -
  QBS03_RS17970 yggU 3855776..3856078 (+) 303 WP_201958626.1 DUF167 family protein YggU -
  QBS03_RS17975 - 3856122..3856541 (+) 420 WP_279486144.1 DUF4426 domain-containing protein -
  QBS03_RS17980 - 3856642..3857838 (-) 1197 WP_005348874.1 NupC/NupG family nucleoside CNT transporter -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38273.03 Da        Isoelectric Point: 6.7440

>NTDB_id=814697 QBS03_RS17950 WP_005340354.1 3852471..3853505(-) (pilT) [Aeromonas veronii strain ANYA 15110]
MDITELLAFSVKHKASDLHLSAGVPPMIRVDGEVRKINLPALEHREVHALIYDIMNDHQRKELEENFEVDFSFEVPNLAR
FRVNAFQQARGSGAVFRTIPSTVLSLEDLDAPEIFRKIAEFPRGLVLVTGPTGSGKSTTLAAMVNYINENFHHHILTIED
PIEFVHENKRCLVNQREVHRDTKSFSNALRSALREDPDIILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGAEKDMVRSMLSESLRAVISQTLLKRIGGGRVAAHEIMMGIPAVRNLIREDKIAQLYSVIQTGMTHGMQTMDQSL
KQLVSRGVVASLDAKAKAVDPNSI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=814697 QBS03_RS17950 WP_005340354.1 3852471..3853505(-) (pilT) [Aeromonas veronii strain ANYA 15110]
ATGGATATCACAGAGTTATTGGCTTTCAGTGTAAAGCATAAAGCCTCGGATCTACACCTCTCGGCCGGGGTTCCCCCGAT
GATCAGGGTTGATGGTGAGGTTCGCAAGATCAATTTGCCCGCCCTGGAACACCGGGAAGTGCATGCCCTCATTTACGACA
TCATGAACGACCATCAGCGCAAGGAGCTGGAGGAGAACTTCGAGGTCGACTTCTCGTTCGAGGTGCCCAATCTGGCCCGT
TTCCGGGTCAACGCCTTCCAGCAGGCTCGCGGCTCGGGCGCGGTATTTCGTACCATCCCCAGCACGGTGCTGAGCCTCGA
AGATCTCGATGCGCCGGAGATCTTCCGCAAGATCGCCGAGTTCCCGCGGGGTTTGGTGTTGGTGACCGGCCCGACCGGTT
CCGGTAAGTCGACCACCCTGGCAGCCATGGTCAACTACATCAACGAAAACTTCCATCACCACATTCTCACCATCGAAGAC
CCCATCGAATTCGTCCACGAGAACAAGCGCTGTCTGGTGAACCAGCGGGAAGTGCATCGGGATACCAAGAGCTTTAGCAA
CGCTCTGCGCTCGGCATTGCGGGAAGACCCGGACATCATTCTGGTGGGCGAGATGCGCGACCTTGAGACCATTCGTTTGG
CCATGACCGCCGCCGAAACTGGCCATCTGGTGTTTGGCACCCTGCACACCTCGTCGGCGGCCAAGACCATCGACCGTATC
ATTGACGTCTTCCCCGGCGCGGAGAAGGACATGGTGCGTTCCATGCTCTCCGAATCCCTGCGGGCAGTTATCTCCCAGAC
CCTGCTCAAGCGTATTGGCGGTGGCCGGGTTGCGGCCCACGAGATCATGATGGGCATTCCGGCGGTGCGTAACCTCATTC
GGGAGGACAAGATTGCCCAGCTCTACTCGGTGATCCAGACCGGGATGACCCACGGCATGCAGACCATGGATCAGAGCCTC
AAGCAGCTGGTGAGTCGTGGCGTGGTGGCATCCCTCGATGCCAAGGCCAAGGCTGTTGATCCCAACAGCATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

77.326

100

0.773
  pilT Acinetobacter baylyi ADP1

77.551

99.709

0.773
  pilT Acinetobacter baumannii D1279779

77.035

100

0.77
  pilT Acinetobacter baumannii strain A118

77.035

100

0.77
  pilT Pseudomonas stutzeri DSM 10701

76.163

100

0.762
  pilT Pseudomonas aeruginosa PAK

75.291

100

0.753
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

75.964

97.965

0.744
  pilT Vibrio cholerae strain A1552

75.964

97.965

0.744
  pilT Legionella pneumophila strain Lp02

72.941

98.837

0.721
  pilT Legionella pneumophila strain ERS1305867

72.941

98.837

0.721
  pilT Neisseria meningitidis 8013

69.855

100

0.701
  pilT Neisseria gonorrhoeae MS11

69.565

100

0.698
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.754

99.419

0.515
  pilU Vibrio cholerae strain A1552

42.899

100

0.43
  pilU Pseudomonas stutzeri DSM 10701

40.58

100

0.407
  pilU Acinetobacter baylyi ADP1

40.462

100

0.407