Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   RYX44_RS10015 Genome accession   NZ_CP136432
Coordinates   2095007..2096515 (+) Length   502 a.a.
NCBI ID   WP_003158838.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain XM8     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2090007..2101515
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RYX44_RS09995 - 2090037..2091248 (+) 1212 WP_003113933.1 O-succinylhomoserine sulfhydrylase -
  RYX44_RS10000 - 2091245..2092012 (+) 768 WP_003103539.1 SDR family oxidoreductase -
  RYX44_RS10005 xcpQ 2092099..2094075 (-) 1977 WP_003103536.1 GspD family T2SS secretin variant XcpQ -
  RYX44_RS10010 xcpP 2094080..2094787 (-) 708 WP_049876919.1 type II secretion system protein N -
  RYX44_RS10015 pilB 2095007..2096515 (+) 1509 WP_003158838.1 GspE family T2SS ATPase variant XcpR Machinery gene
  RYX44_RS10020 xcpS 2096515..2097732 (+) 1218 WP_003091376.1 GspF family T2SS innner membrane protein variant XcpS -
  RYX44_RS10025 xcpT 2097737..2098183 (+) 447 WP_003119751.1 GspG family T2SS major pseudopilin variant XcpT -
  RYX44_RS10030 xcpU 2098190..2098708 (+) 519 WP_003103530.1 GspH family T2SS minor pseudopilin variant XcpU -
  RYX44_RS10035 xcpV 2098705..2099094 (+) 390 WP_003103528.1 GspI family T2SS minor pseudopilin variant XcpV -
  RYX44_RS10040 xcpW 2099091..2099804 (+) 714 WP_023084094.1 GspJ family T2SS minor pseudopilin variant XcpW -
  RYX44_RS10045 xcpX 2099801..2100802 (+) 1002 WP_003103524.1 GspK family T2SS minor pseudopilin variant XcpX -

Sequence

Protein


Download         Length: 502 a.a.        Molecular weight: 55552.74 Da        Isoelectric Point: 6.2074

>NTDB_id=814640 RYX44_RS10015 WP_003158838.1 2095007..2096515(+) (pilB) [Pseudomonas aeruginosa strain XM8]
MMTAPLPDIAAPAAPPRRLPFSFAKRQGLLFLCLEEQYWLACRPQVELAAIAEAQRFAGRRLPLRALGEDAFNQALAASY
QHDSSAAMQLAEDLGGSLDLAALADQVPETEDLMEQEDDAPIIRLINAILGEAIRENASDIHLETFEKRLVVRFRVDGVL
REVLEPKRELAALLVSRIKVMARLDIAEKRIPQDGRISLRVGGREVDIRVSTLPSANGERVVLRLLDKQAGRLNLQHLGM
SERDRKLMDETVRKPHGILLVTGPTGSGKTTTLYASLTTLNDRTRNILTVEDPIEYHLEGIGQTQVNAKVDMTFARGLRA
ILRQDPDVVMVGEIRDRETAEIAVQASLTGHLVLSTLHTNSAIGAITRLVDMGIEPFLLSSSMLGVLAQRLVRVLCPACK
EPYRADEAECALLGVDPAAPPTLHRARGCGECHQHGYRGRTGIYELVVFDDHMRSLIHNESSEQEMTRHARTSGPSIRDD
GRRKVLEGVTTVEEVLRVTREE

Nucleotide


Download         Length: 1509 bp        

>NTDB_id=814640 RYX44_RS10015 WP_003158838.1 2095007..2096515(+) (pilB) [Pseudomonas aeruginosa strain XM8]
ATGATGACAGCCCCATTACCCGACATCGCCGCGCCGGCCGCACCGCCACGGCGTCTGCCGTTCAGCTTCGCCAAGCGCCA
GGGCCTGTTGTTCCTGTGCCTGGAGGAGCAGTACTGGCTGGCCTGCCGGCCGCAGGTGGAGCTGGCGGCGATCGCCGAGG
CGCAGCGCTTCGCCGGCCGCCGCCTGCCGCTCAGGGCGCTCGGCGAGGACGCCTTCAACCAGGCCCTGGCGGCCTCCTAC
CAGCACGACTCCTCGGCGGCCATGCAGCTCGCCGAGGACCTCGGCGGTAGCCTGGACCTCGCCGCGCTGGCCGACCAGGT
CCCGGAAACCGAGGACCTGATGGAGCAGGAGGACGACGCGCCGATCATTCGCCTGATCAACGCGATCCTCGGCGAGGCGA
TCCGCGAGAACGCCTCGGACATCCACCTGGAAACCTTCGAGAAACGCCTGGTGGTGCGCTTCCGCGTCGACGGCGTGCTG
CGCGAGGTGCTGGAGCCCAAGCGCGAGCTGGCGGCCCTGCTGGTATCGCGGATCAAGGTCATGGCGCGGCTGGACATCGC
CGAGAAGCGTATTCCCCAGGATGGGCGGATTTCCCTGCGGGTCGGCGGGCGCGAGGTGGACATCCGCGTCTCGACCCTGC
CCTCGGCCAACGGTGAACGGGTGGTGCTGCGTCTGCTCGACAAGCAGGCCGGGCGCCTGAACCTGCAACACCTGGGCATG
AGCGAGCGCGACCGCAAGCTGATGGACGAAACGGTGCGCAAGCCGCACGGCATCCTGCTGGTCACCGGCCCCACCGGCTC
GGGCAAGACCACCACCCTGTACGCCAGCCTGACCACGCTCAACGACCGCACGCGGAACATCCTCACCGTCGAGGATCCGA
TCGAATACCACCTGGAGGGCATCGGCCAGACCCAGGTCAACGCCAAGGTCGACATGACCTTCGCCCGCGGCCTGCGGGCG
ATCCTCCGGCAGGACCCGGACGTGGTGATGGTCGGCGAGATCCGCGACCGGGAGACCGCCGAGATCGCGGTGCAGGCTTC
GCTGACCGGCCACCTGGTGCTCTCGACCCTGCACACCAACAGCGCCATCGGCGCGATCACCCGCCTGGTGGACATGGGCA
TCGAGCCGTTCCTGCTGTCCTCCTCCATGCTTGGCGTGCTGGCCCAGCGCCTGGTGCGGGTGCTCTGCCCGGCGTGCAAG
GAGCCCTACCGCGCCGACGAGGCCGAGTGCGCGCTGCTCGGCGTCGATCCCGCCGCGCCGCCGACCCTGCATCGCGCCCG
CGGCTGCGGCGAGTGCCACCAGCATGGCTATCGCGGGCGTACCGGTATCTATGAGCTGGTGGTGTTCGACGACCACATGC
GCTCGCTGATCCACAACGAATCCTCCGAGCAGGAAATGACCCGGCATGCCCGCACCAGCGGACCGAGCATCCGCGACGAC
GGTCGGCGCAAGGTGCTGGAAGGCGTCACTACGGTCGAGGAAGTCCTGCGCGTGACCCGGGAAGAATAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(128-394)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

43.737

97.012

0.424
  pilB/pilB1 Synechocystis sp. PCC 6803

42.245

97.61

0.412
  pilB Vibrio campbellii strain DS40M4

43.949

93.825

0.412
  pilB Vibrio parahaemolyticus RIMD 2210633

47.045

84.263

0.396
  pilF Thermus thermophilus HB27

43.146

88.645

0.382
  pilB Acinetobacter baumannii D1279779

47.88

79.88

0.382
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.446

76.892

0.373
  pilF Neisseria gonorrhoeae MS11

46.954

78.486

0.369
  pilB Acinetobacter baylyi ADP1

47.68

77.291

0.369
  pilB Legionella pneumophila strain ERS1305867

47.423

77.291

0.367