Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   RWA07_RS03275 Genome accession   NZ_CP136183
Coordinates   700181..701671 (+) Length   496 a.a.
NCBI ID   WP_001166321.1    Uniprot ID   V5VGM1
Organism   Acinetobacter baumannii strain ABO21-A001     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 695181..706671
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RWA07_RS03260 (RWA07_03260) - 695545..698346 (+) 2802 WP_002125985.1 DUF2339 domain-containing protein -
  RWA07_RS03265 (RWA07_03265) - 698336..699253 (-) 918 WP_000785635.1 mechanosensitive ion channel family protein -
  RWA07_RS03270 (RWA07_03270) - 699263..700084 (-) 822 WP_000462729.1 hypothetical protein -
  RWA07_RS03275 (RWA07_03275) pilB 700181..701671 (+) 1491 WP_001166321.1 type II secretion system ATPase GspE Machinery gene
  RWA07_RS03280 (RWA07_03280) - 701726..702157 (-) 432 WP_000051124.1 organic hydroperoxide resistance protein -
  RWA07_RS03285 (RWA07_03285) - 702245..702682 (-) 438 WP_000371731.1 MarR family winged helix-turn-helix transcriptional regulator -
  RWA07_RS03290 (RWA07_03290) - 702843..703220 (-) 378 WP_000266120.1 DUF4951 domain-containing protein -
  RWA07_RS03295 (RWA07_03295) - 703235..704059 (-) 825 WP_004836080.1 carbon-nitrogen hydrolase family protein -
  RWA07_RS03300 (RWA07_03300) gigB 704086..704607 (-) 522 WP_000103065.1 anti-anti-sigma factor GigB -
  RWA07_RS03305 (RWA07_03305) gigA 704710..705678 (-) 969 WP_000273589.1 RsbU family protein phosphatase GigA -

Sequence

Protein


Download         Length: 496 a.a.        Molecular weight: 55329.22 Da        Isoelectric Point: 6.2037

>NTDB_id=813895 RWA07_RS03275 WP_001166321.1 700181..701671(+) (pilB) [Acinetobacter baumannii strain ABO21-A001]
MQILKQIQVPYSFAKRHGVLFRYDGDQVFIVRRQNTEKIALQEARRILGKPAHYQLCTEQEFNSLLSTSYAGDTGESQQV
AAGLEDHPDLLSLADQVPETEDLMDQEDDAPIVRLINALLSEAIRVGASDIHIEAFEKKLSVRLRVDGQLREIVQPRREL
APLLVSRIKVMAKLDIAEKRVPQDGRISLRLAGREVDVRVSTLPSSHGERVVMRLLDKQAGRLNMTHLGLMANDYERLTQ
LVHRPHGIILVTGPTGSGKTTTLYAALSDLNDNTRNILTAEDPIEYQLEGIGQTQVNTKVDMTFARALKAMLRQDPDVVM
VGEIRDLETAEIAVQASLTGHLVLSTLHTNTAIGAVTRLKDMGIEPFLLSSSLIGVVAQRLVRTLCPHCMTWREADTFEK
QVFQHISHEPSLKLPEAQGCDQCSHLGFNGRTAIYEIVPIDEPMRRLIHGNAAEFELENHARQYSGSIRDDGLRKVLSGK
TTLEEVLRVTNEASEA

Nucleotide


Download         Length: 1491 bp        

>NTDB_id=813895 RWA07_RS03275 WP_001166321.1 700181..701671(+) (pilB) [Acinetobacter baumannii strain ABO21-A001]
ATGCAAATATTGAAACAAATACAAGTTCCATATAGTTTTGCCAAGCGTCATGGTGTTTTATTTCGTTATGACGGTGATCA
AGTTTTTATTGTTAGACGTCAAAATACAGAAAAAATTGCATTGCAGGAAGCAAGACGAATTTTGGGGAAACCTGCACACT
ATCAATTATGCACTGAACAAGAATTTAATAGTCTATTAAGTACCAGTTATGCAGGTGATACAGGTGAATCTCAACAAGTA
GCTGCAGGCTTAGAAGACCATCCGGATTTATTAAGCCTTGCAGATCAGGTGCCTGAAACAGAAGACTTAATGGATCAAGA
GGATGACGCGCCTATTGTTCGTCTAATCAATGCACTTCTCTCTGAGGCAATTCGTGTTGGAGCTTCAGATATCCATATTG
AAGCCTTTGAGAAAAAGCTATCAGTTCGTTTACGGGTAGATGGTCAATTACGTGAAATTGTGCAGCCACGCCGTGAACTA
GCCCCGTTACTTGTTTCGCGTATTAAAGTTATGGCGAAACTCGATATTGCTGAAAAGCGTGTACCGCAAGATGGACGTAT
TTCTTTACGTCTTGCTGGTCGTGAAGTCGATGTCCGTGTTTCAACTTTGCCATCGTCACATGGTGAAAGGGTGGTCATGC
GTTTACTCGATAAACAAGCGGGTCGCCTTAATATGACTCACCTAGGTTTAATGGCAAATGACTATGAACGTTTAACTCAG
TTAGTACATCGTCCGCACGGTATTATTTTGGTCACAGGGCCTACTGGTTCGGGGAAAACGACGACTTTATATGCTGCCTT
ATCTGATCTAAATGACAATACCCGTAACATCTTAACCGCCGAAGATCCAATTGAGTATCAGCTTGAAGGTATTGGCCAAA
CTCAAGTGAATACCAAAGTGGATATGACATTTGCTCGTGCTTTAAAGGCAATGTTACGTCAAGATCCAGATGTGGTCATG
GTGGGGGAGATTCGTGATCTGGAAACTGCTGAAATTGCGGTTCAAGCATCTTTAACAGGTCATTTGGTTTTATCGACGCT
ACATACCAATACTGCTATTGGTGCAGTGACTCGTCTTAAAGATATGGGGATTGAACCCTTCTTATTATCAAGTTCTTTAA
TTGGGGTGGTTGCGCAGCGTTTGGTTCGCACTTTATGTCCACATTGTATGACATGGCGTGAAGCCGATACTTTTGAGAAG
CAAGTTTTTCAGCATATTTCCCATGAACCAAGTTTAAAGTTACCAGAGGCTCAAGGGTGTGATCAGTGTTCTCATTTAGG
GTTTAATGGCCGTACAGCGATTTATGAAATTGTACCTATTGATGAACCTATGCGCCGTCTCATTCATGGTAATGCAGCTG
AATTTGAATTAGAAAATCATGCTCGTCAATATTCTGGTTCTATTCGCGATGATGGTTTACGTAAGGTGCTCTCAGGTAAA
ACTACACTTGAAGAAGTGCTTCGTGTAACCAATGAAGCAAGTGAAGCATAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(118-384)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB V5VGM1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

41.598

98.387

0.409
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.057

99.194

0.407
  pilF Thermus thermophilus HB27

39.351

99.395

0.391
  pilB Vibrio campbellii strain DS40M4

48.371

80.444

0.389
  pilB/pilB1 Synechocystis sp. PCC 6803

40.041

97.177

0.389
  pilB Vibrio parahaemolyticus RIMD 2210633

48

80.645

0.387
  pilB Vibrio cholerae strain A1552

48

80.645

0.387
  pilF Neisseria gonorrhoeae MS11

48.477

79.435

0.385
  pilB Acinetobacter baumannii D1279779

47.146

81.25

0.383
  pilB Acinetobacter baylyi ADP1

46.787

78.427

0.367