Detailed information NTG0812169

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: P9972_RS12675
• Genome accession: NZ_CP121465
• Coordinates: 2591687..2592301 (-)
• Length: 204 a.a.
• NCBI ID: WP_003152867.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain YA215
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2586687..2597301

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
P9972_RS12645 (P9972_12645)	rpsT	2586987..2587253 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
P9972_RS12650 (P9972_12650)	holA	2587270..2588311 (-)	1042	Protein_2446	DNA polymerase III subunit delta	-
P9972_RS12655 (P9972_12655)	-	2588351..2588505 (-)	155	Protein_2447	hypothetical protein	-
P9972_RS12660 (P9972_12660)	-	2588546..2588680 (+)	135	WP_003152870.1	YqzM family protein	-
P9972_RS12665 (P9972_12665)	comEC	2588699..2591050 (-)	2352	WP_041481887.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
P9972_RS12670 (P9972_12670)	-	2591051..2591620 (-)	570	WP_003152868.1	ComE operon protein 2	-
P9972_RS12675 (P9972_12675)	comEA	2591687..2592301 (-)	615	WP_003152867.1	helix-hairpin-helix domain-containing protein	Machinery gene
P9972_RS12680 (P9972_12680)	comER	2592360..2593181 (+)	822	WP_014305449.1	late competence protein ComER	-
P9972_RS12685 (P9972_12685)	-	2593250..2593987 (-)	738	WP_032856885.1	class I SAM-dependent methyltransferase	-
P9972_RS12690 (P9972_12690)	rsfS	2593984..2594340 (-)	357	WP_003152864.1	ribosome silencing factor	-
P9972_RS12695 (P9972_12695)	yqeK	2594358..2594918 (-)	561	WP_032856887.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
P9972_RS12700 (P9972_12700)	-	2594908..2595477 (-)	570	WP_003152860.1	nicotinate-nucleotide adenylyltransferase	-
P9972_RS12705 (P9972_12705)	yhbY	2595488..2595778 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
P9972_RS12710 (P9972_12710)	aroE	2595772..2596614 (-)	843	WP_003152856.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22269.48 Da
• Isoelectric Point: 6.4940

>NTDB_id=812169 P9972_RS12675 WP_003152867.1 2591687..2592301(-) (comEA) [Bacillus velezensis strain YA215]
MTKAMMTERMRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAVGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMKVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=812169 P9972_RS12675 WP_003152867.1 2591687..2592301(-) (comEA) [Bacillus velezensis strain YA215]
ATGACGAAAGCGATGATGACGGAGCGGATGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCAGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGTCAAACAGCCTCCGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGAGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGTTGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAAGGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	59.375	94.118	0.559
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA	Latilactobacillus sakei subsp. sakei 23K	31.897	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363