Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ROT04_RS09675 Genome accession   NZ_CP135174
Coordinates   2051142..2052650 (+) Length   502 a.a.
NCBI ID   WP_003091377.1    Uniprot ID   A0A0H2ZC25
Organism   Pseudomonas aeruginosa strain PARM_L1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2046142..2057650
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ROT04_RS09655 (ROT04_09655) - 2046167..2047378 (+) 1212 WP_031692600.1 O-succinylhomoserine sulfhydrylase -
  ROT04_RS09660 (ROT04_09660) - 2047375..2048142 (+) 768 WP_023106180.1 SDR family oxidoreductase -
  ROT04_RS09665 (ROT04_09665) xcpQ 2048237..2050210 (-) 1974 WP_033956828.1 GspD family T2SS secretin variant XcpQ -
  ROT04_RS09670 (ROT04_09670) xcpP 2050215..2050922 (-) 708 WP_042173362.1 type II secretion system protein N -
  ROT04_RS09675 (ROT04_09675) pilB 2051142..2052650 (+) 1509 WP_003091377.1 GspE family T2SS ATPase variant XcpR Machinery gene
  ROT04_RS09680 (ROT04_09680) xcpS 2052650..2053867 (+) 1218 WP_003091376.1 GspF family T2SS innner membrane protein variant XcpS -
  ROT04_RS09685 (ROT04_09685) xcpT 2053872..2054318 (+) 447 WP_003130409.1 GspG family T2SS major pseudopilin variant XcpT -
  ROT04_RS09690 (ROT04_09690) xcpU 2054325..2054843 (+) 519 WP_058355155.1 GspH family T2SS minor pseudopilin variant XcpU -
  ROT04_RS09695 (ROT04_09695) xcpV 2054840..2055229 (+) 390 WP_023108527.1 GspI family T2SS minor pseudopilin variant XcpV -
  ROT04_RS09700 (ROT04_09700) xcpW 2055226..2055939 (+) 714 WP_023108526.1 GspJ family T2SS minor pseudopilin variant XcpW -
  ROT04_RS09705 (ROT04_09705) xcpX 2055936..2056937 (+) 1002 WP_023436422.1 GspK family T2SS minor pseudopilin variant XcpX -

Sequence

Protein


Download         Length: 502 a.a.        Molecular weight: 55524.73 Da        Isoelectric Point: 6.2074

>NTDB_id=810345 ROT04_RS09675 WP_003091377.1 2051142..2052650(+) (pilB) [Pseudomonas aeruginosa strain PARM_L1]
MMTAPLPDIAAPAAPPRRLPFSFAKRQGLLFLCLEEQYWLACRPQVELAAIAEAQRFAGRRLPLKALGEDAFNQALAASY
QHDSSAAMQLAEDLGGSLDLAALADQVPETEDLMEQEDDAPIIRLINAILGEAIRENASDIHLETFEKRLVVRFRVDGVL
REVLEPKRELAALLVSRIKVMARLDIAEKRIPQDGRISLRVGGREVDIRVSTLPSANGERVVLRLLDKQAGRLNLQHLGM
SERDRKLMDETVRKPHGILLVTGPTGSGKTTTLYASLTTLNDRTRNILTVEDPIEYHLEGIGQTQVNAKVDMTFARGLRA
ILRQDPDVVMVGEIRDRETAEIAVQASLTGHLVLSTLHTNSAIGAITRLVDMGIEPFLLSSSMLGVLAQRLVRVLCPACK
EPYRADEAECALLGVDPAAPPTLHRARGCGECHQHGYRGRTGIYELVVFDDHMRSLIHNESSEQEMTRHARTSGPSIRDD
GRRKVLEGVTTVEEVLRVTREE

Nucleotide


Download         Length: 1509 bp        

>NTDB_id=810345 ROT04_RS09675 WP_003091377.1 2051142..2052650(+) (pilB) [Pseudomonas aeruginosa strain PARM_L1]
ATGATGACAGCCCCGTTACCCGACATCGCCGCGCCGGCCGCACCGCCACGGCGTCTGCCGTTCAGTTTCGCCAAGCGCCA
GGGTCTGCTGTTCCTGTGCCTGGAGGAGCAGTACTGGCTGGCCTGCCGGCCGCAGGTGGAGCTGGCGGCGATCGCCGAGG
CGCAGCGCTTCGCCGGCCGCCGCCTGCCGCTCAAGGCGCTCGGCGAGGATGCCTTCAACCAGGCCCTGGCGGCGTCCTAC
CAGCACGATTCCTCGGCGGCCATGCAGCTCGCCGAGGACCTCGGCGGCAGCCTGGACCTCGCCGCCCTGGCCGACCAGGT
CCCGGAAACCGAGGACCTGATGGAGCAGGAGGACGACGCGCCGATCATTCGCCTGATCAACGCGATCCTCGGCGAGGCGA
TCCGCGAGAACGCTTCGGACATCCACCTGGAAACCTTCGAGAAACGCCTGGTGGTGCGCTTCCGCGTCGACGGCGTACTG
CGCGAGGTGCTCGAACCCAAGCGCGAGCTGGCGGCCTTGCTGGTATCGCGGATCAAGGTCATGGCGCGGCTGGACATCGC
CGAGAAACGCATTCCCCAGGATGGACGGATTTCCCTGCGGGTCGGCGGGCGTGAGGTGGACATCCGCGTCTCGACCCTGC
CCTCGGCCAACGGCGAGCGCGTGGTGCTGCGTCTGCTCGACAAGCAGGCCGGGCGCCTGAACCTGCAGCACCTGGGCATG
AGCGAGCGCGACCGCAAGCTGATGGACGAAACCGTGCGCAAGCCGCACGGCATCCTGCTGGTCACCGGGCCCACCGGTTC
GGGCAAGACCACCACCCTGTACGCCAGCCTGACCACGCTCAACGACCGCACGCGGAACATCCTCACCGTCGAGGATCCGA
TCGAATACCACCTGGAAGGCATCGGCCAGACCCAGGTCAATGCCAAGGTCGACATGACCTTCGCCCGCGGCCTGCGGGCG
ATCCTACGGCAAGACCCGGACGTGGTGATGGTCGGCGAGATCCGCGACCGGGAGACCGCCGAGATCGCGGTGCAGGCTTC
GCTGACCGGCCACCTGGTGCTCTCGACCCTGCACACCAACAGCGCCATCGGCGCGATCACCCGCCTGGTGGACATGGGCA
TCGAGCCGTTCCTGCTGTCCTCCTCCATGCTTGGCGTGCTGGCCCAGCGCCTGGTGCGGGTGCTCTGCCCGGCGTGCAAG
GAGCCCTACCGGGCCGACGAGGCCGAGTGCGCGCTGCTCGGCGTCGATCCCGCCGCGCCGCCGACCCTGCATCGCGCCCG
CGGCTGCGGCGAGTGCCACCAGCATGGCTATCGCGGGCGTACCGGTATCTATGAACTGGTGGTGTTCGACGACCACATGC
GCTCGCTGATCCACAACGAATCCTCCGAGCAGGAAATGACCCGGCATGCCCGCACCAGTGGACCGAGCATCCGCGACGAC
GGTCGGCGCAAGGTGCTGGAAGGCGTCACCACGGTCGAGGAAGTCCTGCGCGTGACCCGGGAAGAATAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(128-394)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2ZC25

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

43.532

97.012

0.422
  pilB/pilB1 Synechocystis sp. PCC 6803

42.245

97.61

0.412
  pilB Vibrio campbellii strain DS40M4

43.249

94.422

0.408
  pilB Vibrio parahaemolyticus RIMD 2210633

47.045

84.263

0.396
  pilF Thermus thermophilus HB27

43.207

89.442

0.386
  pilB Acinetobacter baumannii D1279779

47.88

79.88

0.382
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.446

76.892

0.373
  pilF Neisseria gonorrhoeae MS11

46.954

78.486

0.369
  pilB Acinetobacter baylyi ADP1

47.68

77.291

0.369
  pilB Legionella pneumophila strain ERS1305867

47.423

77.291

0.367