Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   P8S54_RS02490 Genome accession   NZ_CP121119
Coordinates   545669..547426 (+) Length   585 a.a.
NCBI ID   WP_281443078.1    Uniprot ID   A0AAX3S7W3
Organism   Halomonas sp. M1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 540669..552426
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P8S54_RS02455 (P8S55_02445) - 540784..540987 (+) 204 WP_281443072.1 hypothetical protein -
  P8S54_RS02460 (P8S55_02450) - 541327..542724 (+) 1398 WP_281443073.1 hypothetical protein -
  P8S54_RS02470 (P8S55_02460) - 543063..543872 (-) 810 WP_281443074.1 inositol monophosphatase family protein -
  P8S54_RS02475 (P8S55_02465) - 543959..544420 (-) 462 WP_281443075.1 anti-virulence regulator CigR family protein -
  P8S54_RS02480 (P8S55_02470) - 544513..544905 (-) 393 WP_281443076.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  P8S54_RS02485 (P8S55_02475) - 544981..545406 (-) 426 WP_281443077.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  P8S54_RS02490 (P8S55_02480) pilB 545669..547426 (+) 1758 WP_281443078.1 type IV-A pilus assembly ATPase PilB Machinery gene
  P8S54_RS02495 (P8S55_02485) - 547435..548664 (+) 1230 WP_281443079.1 type II secretion system F family protein -
  P8S54_RS02500 (P8S55_02490) pilD 548717..549562 (+) 846 WP_281443080.1 A24 family peptidase Machinery gene
  P8S54_RS02505 (P8S55_02495) coaE 549697..550293 (+) 597 WP_281443081.1 dephospho-CoA kinase -
  P8S54_RS02510 (P8S55_02500) yacG 550309..550542 (+) 234 WP_009095454.1 DNA gyrase inhibitor YacG -
  P8S54_RS02515 (P8S55_02505) - 550559..551431 (+) 873 WP_281443082.1 DNA replication terminus site-binding protein -
  P8S54_RS02520 (P8S55_02510) - 551428..551763 (-) 336 WP_281443083.1 hypothetical protein -

Sequence

Protein


Download         Length: 585 a.a.        Molecular weight: 63749.98 Da        Isoelectric Point: 5.9689

>NTDB_id=810208 P8S54_RS02490 WP_281443078.1 545669..547426(+) (pilB) [Halomonas sp. M1]
MRQPAPESTLSHAALNGGLRGIAERLVKHGSLTNAQAAVAESTAAELDISLLQHVVESGLVDANAAALAAAWEYGLPVID
LDSVRINALPPANDYPVKILEKLDILPLARHGHRLTVAVPYPATLTQLDELQFATGLSVDGVLAPADQLKTTLIQYLAQN
ERSMLDELENVDDAVSALNVVHNADGEEIALDNANENSEDAPVVKFVNKILLDAISRGASDIHFEPYETLYRVRFRIDGM
LIEIARPPFALRHRIAARLKIMSRLDISERRLPQDGAIKLQLSRTRSIDFRVNSLPTVYGEKVVLRILDPASAQLGIDQL
GFNPVQQETYESALKKPQGMILVTGPTGSGKTVTLYTGINILNQIERNICTAEDPVEIKVSGINQVNVLPKIGLNFASAL
RAFLRQDPDVVMVGEIRDLETAEIAVKAAQTGHLVLSTVHTNSAAETLTRLANMGVASYNIASSVSLIIAQRLARRLCNM
CKTPAEIPAEALRKAGFSQLDIRNATIYQAKGCKQCTQGYKGRIGVYEVLPVSDDMRQLIMRDGNSIEIDQLARKEGHTS
LHQSCLSRVLDGSTSLEEVNRISKE

Nucleotide


Download         Length: 1758 bp        

>NTDB_id=810208 P8S54_RS02490 WP_281443078.1 545669..547426(+) (pilB) [Halomonas sp. M1]
ATGCGTCAACCCGCCCCCGAATCTACTCTTAGCCATGCAGCCTTAAACGGCGGCCTGCGCGGTATTGCTGAAAGGCTGGT
GAAACACGGCTCATTAACCAATGCCCAGGCGGCAGTTGCTGAGTCTACCGCTGCAGAGCTGGACATTTCGCTATTACAGC
ACGTAGTGGAAAGCGGACTGGTGGATGCCAATGCCGCCGCTTTGGCCGCGGCCTGGGAGTACGGCCTACCGGTGATTGAT
CTTGACTCAGTCCGGATTAATGCGTTGCCACCGGCTAATGACTACCCGGTTAAAATTCTCGAAAAGCTGGATATTCTGCC
CCTCGCACGCCACGGTCACCGCTTAACCGTTGCGGTGCCTTACCCGGCGACCCTGACCCAGCTAGACGAGCTGCAGTTTG
CTACCGGCTTAAGTGTGGATGGCGTGCTGGCCCCGGCGGATCAGCTAAAAACCACGCTTATTCAGTACCTAGCACAAAAC
GAGCGGAGTATGCTCGATGAGCTAGAAAATGTTGACGATGCAGTCAGCGCGCTCAACGTTGTGCACAACGCTGACGGTGA
AGAGATCGCGCTTGATAACGCCAATGAAAATAGTGAAGACGCGCCGGTCGTAAAATTTGTCAACAAAATACTGCTGGATG
CGATTAGCCGTGGCGCTTCGGATATTCACTTTGAGCCCTATGAAACTCTATATAGGGTGCGTTTTCGTATTGACGGCATG
CTGATAGAAATAGCACGCCCGCCCTTTGCTCTTCGCCACCGCATTGCCGCACGGCTCAAAATTATGTCGCGGCTCGATAT
TTCGGAACGCCGCCTTCCACAAGATGGGGCGATAAAGCTTCAGCTTTCCCGTACACGCTCGATTGATTTCCGGGTTAACT
CGCTGCCCACCGTCTACGGCGAAAAAGTGGTGCTGCGTATTCTAGACCCGGCATCGGCTCAACTAGGCATTGACCAGCTT
GGCTTTAACCCAGTACAGCAAGAAACGTATGAAAGCGCGCTTAAAAAGCCCCAAGGGATGATTTTGGTCACCGGCCCCAC
CGGTAGCGGTAAAACCGTAACGCTATATACCGGCATTAATATACTCAACCAAATTGAGCGCAATATTTGTACCGCCGAAG
ACCCGGTGGAAATCAAAGTATCGGGCATTAACCAAGTCAACGTACTACCCAAGATTGGTTTAAACTTTGCCAGCGCCTTA
CGGGCATTTTTGCGTCAAGACCCGGATGTGGTGATGGTGGGTGAAATACGCGATCTCGAAACCGCTGAAATAGCGGTAAA
AGCGGCGCAAACCGGCCACTTGGTGCTCTCAACCGTACACACTAACTCGGCCGCGGAAACCCTTACCCGCTTGGCTAACA
TGGGCGTTGCCTCCTACAACATTGCCAGCTCGGTAAGTTTGATTATCGCCCAACGGCTAGCCCGCAGGCTTTGCAATATG
TGTAAAACCCCTGCGGAAATTCCAGCTGAAGCGCTACGTAAGGCAGGGTTTAGCCAGCTTGATATCCGTAACGCCACTAT
CTATCAAGCCAAAGGTTGTAAGCAGTGTACTCAGGGCTATAAAGGACGAATCGGTGTTTATGAGGTACTGCCGGTTTCTG
ATGACATGCGCCAACTTATTATGCGCGATGGTAATTCCATCGAAATTGACCAATTAGCGCGCAAGGAGGGCCATACAAGC
CTCCACCAAAGCTGCTTAAGCCGTGTTTTGGATGGCAGCACCAGTCTTGAAGAAGTTAACCGAATTAGTAAGGAGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(56-161)

Type II/IV secretion system protein (T2SSE)

(209-476)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

53.18

96.752

0.515
  pilB Acinetobacter baumannii D1279779

51.228

97.436

0.499
  pilB Acinetobacter baylyi ADP1

48.714

99.658

0.485
  pilB Vibrio cholerae strain A1552

49.281

95.043

0.468
  pilB Vibrio parahaemolyticus RIMD 2210633

48.49

96.239

0.467
  pilB Vibrio campbellii strain DS40M4

47.193

97.436

0.46
  pilF Neisseria gonorrhoeae MS11

50.415

82.393

0.415
  pilB/pilB1 Synechocystis sp. PCC 6803

40.381

89.744

0.362