Detailed information NTG0810209

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: P8S54_RS02500
• Genome accession: NZ_CP121119
• Coordinates: 548717..549562 (+)
• Length: 281 a.a.
• NCBI ID: WP_281443080.1
• Uniprot ID: A0AAX3SA65
• Organism: Halomonas sp. M1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 543717..554562

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
P8S54_RS02475 (P8S55_02465)	-	543959..544420 (-)	462	WP_281443075.1	anti-virulence regulator CigR family protein	-
P8S54_RS02480 (P8S55_02470)	-	544513..544905 (-)	393	WP_281443076.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
P8S54_RS02485 (P8S55_02475)	-	544981..545406 (-)	426	WP_281443077.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
P8S54_RS02490 (P8S55_02480)	pilB	545669..547426 (+)	1758	WP_281443078.1	type IV-A pilus assembly ATPase PilB	Machinery gene
P8S54_RS02495 (P8S55_02485)	-	547435..548664 (+)	1230	WP_281443079.1	type II secretion system F family protein	-
P8S54_RS02500 (P8S55_02490)	pilD	548717..549562 (+)	846	WP_281443080.1	A24 family peptidase	Machinery gene
P8S54_RS02505 (P8S55_02495)	coaE	549697..550293 (+)	597	WP_281443081.1	dephospho-CoA kinase	-
P8S54_RS02510 (P8S55_02500)	yacG	550309..550542 (+)	234	WP_009095454.1	DNA gyrase inhibitor YacG	-
P8S54_RS02515 (P8S55_02505)	-	550559..551431 (+)	873	WP_281443082.1	DNA replication terminus site-binding protein	-
P8S54_RS02520 (P8S55_02510)	-	551428..551763 (-)	336	WP_281443083.1	hypothetical protein	-
P8S54_RS02525 (P8S55_02515)	-	552069..552902 (+)	834	WP_281443084.1	helix-turn-helix transcriptional regulator	-
P8S54_RS02530 (P8S55_02520)	-	552994..553365 (-)	372	WP_281443085.1	histone-like nucleoid-structuring protein, MvaT/MvaU family	-

Sequence

Protein

• Length: 281 a.a.
• Molecular weight: 30598.91 Da
• Isoelectric Point: 8.5711

>NTDB_id=810209 P8S54_RS02500 WP_281443080.1 548717..549562(+) (pilD) [Halomonas sp. M1]
MGFPPTLLYLFALLIGLCFGSFLNVVITRLPVMLMRSWRNEARAALELPAETSPRFNLATPGSMCPRCEHPIAWHDNLPL
LGWLKRRGKCANCQTPISAQYPLVELMGGLLAVAVVALYGATASALFIYAACLTLLALAVIDFRTYLLPDILTLPLLWAG
LLYQLLFQPIMLPSAVIGAMAGYLILWSFYWLFKLMTGKEGMGFGDFKLLAALGAWVGWPMLPLLLVLSAGIGAIVGLAA
QARSPHLRGQPLPFGPFLALAGWVCLLAGNELMALYTSLLY

Nucleotide

• Length: 846 bp

>NTDB_id=810209 P8S54_RS02500 WP_281443080.1 548717..549562(+) (pilD) [Halomonas sp. M1]
ATGGGCTTTCCGCCAACACTCTTGTACCTCTTCGCGCTACTTATCGGGCTTTGCTTTGGCAGTTTTTTAAATGTTGTGAT
CACTCGCCTGCCGGTGATGCTGATGCGTAGCTGGCGCAATGAAGCCCGCGCCGCGCTTGAGCTTCCCGCAGAAACCTCGC
CCCGGTTTAATCTCGCAACACCGGGGTCGATGTGCCCACGCTGCGAACACCCCATTGCATGGCACGACAACCTGCCTTTG
CTCGGCTGGTTAAAACGACGCGGCAAGTGCGCCAACTGCCAGACCCCCATTAGTGCGCAGTATCCGCTGGTAGAGCTTAT
GGGTGGGCTATTAGCAGTCGCTGTCGTAGCGCTTTACGGTGCAACGGCAAGCGCTCTGTTTATTTATGCGGCCTGTTTAA
CGCTGCTTGCTCTAGCCGTGATCGATTTTCGCACGTATCTGCTGCCAGATATTTTAACCCTGCCCCTGCTCTGGGCTGGC
TTGCTCTATCAACTGCTCTTCCAACCTATTATGTTGCCCAGTGCCGTTATTGGCGCTATGGCGGGCTACCTAATACTGTG
GAGCTTTTACTGGCTGTTTAAGCTGATGACCGGCAAAGAGGGCATGGGATTTGGCGACTTCAAGCTGCTAGCGGCGTTAG
GTGCCTGGGTCGGCTGGCCCATGCTGCCGCTGCTGTTGGTGTTATCAGCAGGTATTGGCGCTATTGTTGGCTTAGCGGCA
CAAGCACGCTCCCCTCATTTACGCGGCCAGCCACTACCTTTTGGCCCTTTTTTAGCCCTGGCGGGGTGGGTTTGTCTGCT
AGCGGGCAATGAGCTGATGGCGCTTTATACATCGTTGCTTTATTAG

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (14-117)

• Type IV leader peptidase family (Peptidase_A24) (131-238)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	50.896	99.288	0.505
pilD	Vibrio campbellii strain DS40M4	46.643	100	0.47
pilD	Acinetobacter baumannii D1279779	46.931	98.577	0.463
pilD	Acinetobacter nosocomialis M2	46.931	98.577	0.463
pilD	Neisseria gonorrhoeae MS11	44.487	93.594	0.416