Detailed information NTG0808105

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: P4B24_RS26945
• Genome accession: NZ_CP120708
• Coordinates: 5678211..5679911 (+)
• Length: 566 a.a.
• NCBI ID: WP_003122078.1
• Uniprot ID: -
• Organism: Pseudomonas aeruginosa strain NY7723
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	5665931..5677233	5678211..5679911	flank	978

Gene organization within MGE regions

Location: 5665931..5679911

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
P4B24_RS26860 (P4B24_26865)	-	5665931..5667145 (+)	1215	WP_023109340.1	site-specific integrase	-
P4B24_RS26865 (P4B24_26870)	-	5667137..5667883 (-)	747	WP_023109341.1	hypothetical protein	-
P4B24_RS26870 (P4B24_26875)	-	5668039..5668239 (+)	201	WP_023109342.1	helix-turn-helix domain-containing protein	-
P4B24_RS26875 (P4B24_26880)	-	5668646..5668879 (+)	234	WP_023109344.1	hypothetical protein	-
P4B24_RS26880 (P4B24_26885)	-	5668876..5669145 (+)	270	WP_003096138.1	hypothetical protein	-
P4B24_RS26885 (P4B24_26890)	-	5669142..5669468 (+)	327	WP_126634418.1	hypothetical protein	-
P4B24_RS26890 (P4B24_26895)	-	5669471..5669761 (+)	291	WP_023109346.1	hypothetical protein	-
P4B24_RS26895 (P4B24_26900)	-	5669758..5670132 (+)	375	WP_023109347.1	hypothetical protein	-
P4B24_RS26900 (P4B24_26905)	-	5670129..5671679 (+)	1551	WP_023109348.1	DUF3631 domain-containing protein	-
P4B24_RS26905 (P4B24_26910)	-	5671679..5671975 (+)	297	WP_023109349.1	hypothetical protein	-
P4B24_RS26910 (P4B24_26915)	-	5671972..5672202 (+)	231	WP_023109350.1	helix-turn-helix domain-containing protein	-
P4B24_RS26915 (P4B24_26920)	-	5672317..5672529 (+)	213	WP_023109351.1	hypothetical protein	-
P4B24_RS26920 (P4B24_26925)	-	5672532..5674373 (+)	1842	WP_023109352.1	ClpP-like prohead protease/major capsid protein fusion protein	-
P4B24_RS26925 (P4B24_26930)	-	5675554..5675796 (+)	243	WP_023109353.1	hypothetical protein	-
P4B24_RS26935 (P4B24_26940)	-	5676055..5677440 (-)	1386	WP_003122080.1	O-antigen ligase	-
P4B24_RS26940 (P4B24_26945)	-	5677516..5677980 (-)	465	WP_003122079.1	pilin	-
P4B24_RS26945 (P4B24_26950)	pilB	5678211..5679911 (+)	1701	WP_003122078.1	type IV-A pilus assembly ATPase PilB	Machinery gene

Sequence

Protein

• Length: 566 a.a.
• Molecular weight: 62299.21 Da
• Isoelectric Point: 5.4470

>NTDB_id=808105 P4B24_RS26945 WP_003122078.1 5678211..5679911(+) (pilB) [Pseudomonas aeruginosa strain NY7723]
MNDSTPLSGLAKQLVLNELLDEKTARQAQVQAARNKQSLVSHLVQAKLVQSRALVEVAAEQFGIAYCDLNSLDRESIPKD
VISERLVRQHRVLPLWKRGNKLFIGLSDPTNHQAVTDIQFSTGLTTEALLVEDDKLGVAIEKLYEGAMDALGDLGDIDLD
GVDVGKAESASQDDGGDDAEDAPVVRFVNKMLLDAIRGGSSDLHFEPYEKTYRVRFRTDGMLHEVAKPPIQLASRISARL
KVMAGLDISERRKPQDGRIKMRVSKTKSIDFRVNTLPTLWGEKIVMRILDSSSAQMGIDALGYEEDQKELYLAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTTDINISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLLNMGVPAFNLATSVNLIIAQRLARKLCSHCKKEHDVPKETLLHEGFPE
ELIGTFKLYSPVGCENCKGGYKGRVGIYEVVKNTPALQRIIMEEGNSIEIAEQARKEGFNDLRTSGLLKAMQGITSLEEV
NRVTKD

Nucleotide

• Length: 1701 bp

>NTDB_id=808105 P4B24_RS26945 WP_003122078.1 5678211..5679911(+) (pilB) [Pseudomonas aeruginosa strain NY7723]
ATGAACGATTCCACGCCTCTCAGCGGCCTTGCCAAGCAACTGGTCCTGAACGAACTCCTCGACGAAAAGACCGCCCGCCA
GGCCCAGGTGCAGGCCGCGCGCAACAAGCAGTCGCTGGTCAGCCACCTGGTCCAGGCCAAGCTGGTGCAGAGCCGGGCGT
TGGTCGAGGTGGCCGCCGAGCAGTTCGGCATCGCCTATTGCGACCTGAACAGCCTGGATCGGGAAAGCATTCCGAAGGAT
GTCATCAGCGAGCGCCTGGTCCGCCAGCACCGTGTGTTGCCGCTGTGGAAACGCGGCAACAAACTGTTCATCGGTCTCTC
CGACCCGACCAACCACCAGGCGGTCACCGATATCCAGTTCAGCACCGGTCTCACCACCGAAGCCCTGCTGGTGGAGGACG
ACAAGCTCGGCGTAGCCATCGAGAAGCTCTACGAGGGCGCTATGGACGCTCTCGGCGACCTCGGTGACATCGATCTCGAC
GGGGTCGATGTCGGCAAGGCGGAAAGCGCCAGCCAGGACGACGGCGGCGACGATGCCGAAGATGCCCCGGTGGTGCGCTT
CGTCAACAAGATGCTGCTCGACGCCATCCGCGGCGGCTCTTCCGACCTGCACTTCGAGCCCTACGAAAAGACCTACCGGG
TGCGCTTCCGTACCGACGGCATGCTCCACGAAGTGGCCAAGCCGCCGATCCAGTTGGCCAGCCGCATTTCCGCGCGACTC
AAGGTAATGGCCGGCCTGGATATCTCCGAACGGCGCAAGCCGCAGGACGGGCGGATCAAGATGCGCGTGTCGAAGACCAA
GTCGATCGACTTCCGCGTCAACACCCTGCCGACCCTGTGGGGCGAGAAGATCGTGATGCGGATCCTCGACTCCTCCAGCG
CGCAGATGGGCATCGACGCACTGGGCTACGAAGAGGACCAGAAGGAACTCTACCTGGCCGCGCTCAAGCAGCCACAGGGC
ATGATCCTGGTCACCGGCCCCACCGGCTCGGGCAAGACGGTTTCGCTGTACACCGGTCTGAACATCCTCAATACCACCGA
CATCAACATCTCCACCGCTGAAGACCCTGTGGAGATCAACCTGGAAGGCATCAACCAGGTCAACGTCAACCCGCGTCAGG
GCATGGACTTCTCCCAGGCGCTGCGCGCCTTCCTGCGCCAGGACCCGGACGTGATCATGGTCGGCGAGATCCGCGATCTG
GAGACTGCCGAGATCGCCATCAAGGCGGCGCAGACCGGGCATATGGTGATGTCCACCCTGCACACCAACAGCGCCGCCGA
GACCCTGACCCGCCTGCTGAACATGGGCGTGCCGGCGTTCAACCTGGCGACCTCGGTGAACCTGATCATCGCCCAGCGCC
TTGCGCGAAAACTCTGTTCGCACTGCAAGAAAGAACACGACGTGCCGAAGGAAACCCTGCTTCACGAAGGTTTCCCGGAA
GAGCTGATCGGCACCTTCAAGCTGTATTCGCCTGTAGGCTGCGAAAACTGCAAAGGCGGTTACAAAGGTCGCGTCGGTAT
TTATGAAGTGGTTAAAAACACCCCCGCCCTGCAGCGGATTATCATGGAAGAAGGCAACTCCATCGAGATCGCCGAGCAAG
CCCGCAAAGAAGGCTTCAACGATCTGCGCACCTCAGGCCTGCTGAAAGCCATGCAGGGAATCACCAGCCTGGAGGAAGTC
AACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (190-457)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (43-146)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Acinetobacter baumannii D1279779	57.544	100	0.58
pilB	Acinetobacter baylyi ADP1	56.79	100	0.569
pilB	Legionella pneumophila strain ERS1305867	54.497	100	0.546
pilB	Vibrio cholerae strain A1552	51.521	98.763	0.509
pilF	Neisseria gonorrhoeae MS11	50.177	99.647	0.5
pilB	Vibrio parahaemolyticus RIMD 2210633	52.158	94.17	0.491
pilB	Vibrio campbellii strain DS40M4	51.22	94.17	0.482
pilB/pilB1	Synechocystis sp. PCC 6803	37.763	100	0.412
pilF	Thermus thermophilus HB27	38.664	100	0.389
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	38.727	97.173	0.376