Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   P4B08_RS28020 Genome accession   NZ_CP120704
Coordinates   6054439..6056586 (-) Length   715 a.a.
NCBI ID   WP_309267272.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain SE5356     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6049439..6061586
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P4B08_RS28005 (P4B08_28020) - 6051097..6052746 (-) 1650 WP_023104793.1 AAA family ATPase -
  P4B08_RS28010 (P4B08_28025) aroB 6052753..6053859 (-) 1107 WP_028681386.1 3-dehydroquinate synthase -
  P4B08_RS28015 (P4B08_28030) aroK 6053909..6054427 (-) 519 WP_003095833.1 shikimate kinase AroK -
  P4B08_RS28020 (P4B08_28035) pilQ 6054439..6056586 (-) 2148 WP_309267272.1 type 4a pilus secretin PilQ Machinery gene
  P4B08_RS28025 (P4B08_28040) pilP 6056640..6057164 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP -
  P4B08_RS28030 (P4B08_28045) pilO 6057161..6057784 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO -
  P4B08_RS28035 (P4B08_28050) pilN 6057781..6058377 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN -
  P4B08_RS28040 (P4B08_28055) pilM 6058377..6059441 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 715 a.a.        Molecular weight: 77552.15 Da        Isoelectric Point: 5.2285

>NTDB_id=807979 P4B08_RS28020 WP_309267272.1 6054439..6056586(-) (pilQ) [Pseudomonas aeruginosa strain SE5356]
MNSGLSRLGIALLAAMFAPALLAADLEKLDVAALPGDRVELKLQFDEPVAAPRGYTIEQPARIALDLPGVQNKLGTKNRE
LSVGNTRSVTVVEAKDRTRLIINLTTLSSYTTRVEGNNLFVVVGNSPAGASVASTAPVKASPAPASYAQPIKPKPYVPAG
RAIRNIDFQRGEKGEGNVVIDLSDPTLSPDIQEQGGKIRLDFAKTQLPDALRVRLDVKDFATPVQFVNASAQSDRTSITI
EPSGLYDYLVYQTDNRLTVSIKPMTTEDAERRKKDNFAYTGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVQGNIIT
LRLQNVPWDQALDLVLKTKGLDKRKLGNVLLVAPADEIAARERQELEAQKQIAELAPLRRELIQVNYAKAADIAKLFQSV
TSDGGQEGKEGGRGSITVDDRTNSIIAYQPQERLDELRRIVSQLDIPVRQVMIEARIVEANVGYDKSLGVRWGGAYHKGN
WSGYGKDGNIGIKDEDGMNCGPIAGSCTFPTTGTSKSPSPFVDLGAKDATSGIGIGFITDNIILDLQLSAMEKTGNGEIV
SQPKVVTSDKETAKILKGSEVPYQEASSSGATSTSFKEAALSLEVTPQITPDNRIIVEVKVTKDAPDYQNMLNGVPPINK
NEVNAKILVNDGETIVIGGVFSNEQSKSVEKVPFLGELPYLGRLFRRDTVTDRKNELLVFLTPRIMNNQAIAIGR

Nucleotide


Download         Length: 2148 bp        

>NTDB_id=807979 P4B08_RS28020 WP_309267272.1 6054439..6056586(-) (pilQ) [Pseudomonas aeruginosa strain SE5356]
ATGAACAGTGGCCTCTCGCGCCTCGGGATCGCTTTGCTGGCCGCCATGTTCGCGCCGGCATTGCTCGCCGCGGACCTGGA
GAAACTCGACGTGGCGGCATTGCCTGGCGACCGGGTCGAGCTAAAGCTGCAGTTCGACGAGCCCGTCGCCGCGCCGCGTG
GCTATACCATCGAGCAGCCGGCGCGAATCGCCCTCGATCTGCCGGGGGTACAGAACAAGCTCGGCACCAAGAACCGCGAG
CTGAGCGTGGGCAATACGCGCAGCGTCACGGTGGTCGAGGCGAAGGATCGCACACGGTTGATCATCAACCTGACCACGTT
GTCGTCCTACACCACCCGGGTGGAAGGCAACAACCTCTTCGTGGTGGTCGGCAATTCGCCGGCCGGCGCCAGCGTCGCTT
CCACCGCTCCGGTCAAGGCAAGCCCGGCTCCGGCCAGCTATGCCCAGCCGATCAAGCCCAAGCCGTACGTGCCGGCGGGT
CGTGCCATTCGCAACATCGATTTCCAGCGTGGCGAAAAGGGCGAGGGCAACGTGGTCATCGACCTGTCCGATCCGACCTT
GAGCCCGGATATCCAGGAGCAGGGCGGCAAGATCCGCCTGGACTTCGCCAAGACCCAACTGCCGGATGCGCTGCGGGTTC
GCCTGGACGTCAAGGACTTCGCCACGCCGGTGCAGTTCGTCAACGCCAGCGCGCAATCCGACCGTACCAGCATCACCATC
GAGCCGAGCGGGCTCTACGACTATCTGGTCTACCAGACCGACAATCGCCTGACCGTCAGCATCAAGCCGATGACCACCGA
AGACGCCGAGCGGCGCAAGAAGGACAATTTCGCCTATACCGGTGAGAAACTGTCGCTGAACTTCCAGGACATCGACGTGC
GTTCGGTACTGCAGCTGATCGCCGACTTCACCGACCTGAACCTGGTGGCCTCGGATACCGTGCAGGGCAATATCATCACC
CTGCGCCTGCAGAACGTGCCGTGGGACCAGGCGCTGGACCTGGTGCTGAAGACCAAGGGCCTGGACAAGCGCAAGCTCGG
CAACGTGCTCCTGGTGGCCCCGGCCGACGAGATCGCCGCCCGTGAGCGCCAGGAGCTGGAAGCGCAGAAGCAGATCGCCG
AGTTGGCGCCACTGCGTCGGGAACTGATCCAGGTGAACTACGCCAAGGCCGCGGACATCGCCAAGCTGTTCCAGTCGGTG
ACCAGCGATGGTGGCCAGGAAGGCAAGGAAGGTGGGCGCGGCTCGATCACCGTCGACGACCGCACCAACAGTATCATCGC
CTATCAGCCCCAGGAGCGACTGGACGAACTGCGGCGGATCGTTTCGCAGCTGGATATTCCGGTGCGCCAGGTGATGATCG
AGGCACGCATCGTCGAGGCCAACGTCGGTTACGACAAGAGTCTCGGTGTGCGCTGGGGCGGCGCTTATCACAAGGGCAAC
TGGAGCGGCTACGGCAAGGACGGCAATATCGGGATCAAGGACGAGGACGGCATGAACTGCGGCCCGATCGCCGGTAGCTG
TACCTTCCCGACCACGGGTACCAGCAAGTCGCCGTCGCCGTTCGTCGACCTCGGTGCCAAGGATGCTACCTCCGGGATCG
GCATCGGCTTCATCACCGACAACATCATCCTCGACCTGCAACTGTCGGCGATGGAAAAGACCGGCAACGGCGAGATCGTC
TCCCAGCCCAAGGTCGTGACCTCGGACAAGGAAACCGCGAAGATCCTGAAGGGCTCGGAAGTCCCCTACCAGGAGGCCAG
TTCCAGCGGCGCTACCAGCACCTCGTTCAAGGAAGCCGCGCTGTCCCTCGAGGTGACCCCGCAGATCACCCCGGACAACC
GGATCATCGTCGAAGTCAAGGTAACCAAGGACGCGCCGGATTACCAGAATATGTTGAACGGCGTACCTCCTATCAACAAG
AACGAGGTCAACGCCAAGATCCTGGTCAACGACGGCGAGACCATCGTCATCGGCGGCGTGTTCAGTAACGAGCAGAGCAA
GTCGGTGGAGAAGGTACCCTTCCTCGGCGAACTGCCTTACCTCGGGCGGTTGTTCCGGCGAGATACCGTCACCGACCGGA
AAAACGAACTTCTGGTTTTCCTGACACCGCGGATCATGAATAATCAGGCCATCGCAATCGGTCGCTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(382-450)

AMIN domain (AMIN)

(38-122)

Bacterial type II and III secretion system protein (Secretin)

(550-706)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

99.86

100

0.999
  pilQ Acinetobacter baumannii D1279779

40.642

100

0.407
  pilQ Acinetobacter baumannii strain A118

40.642

100

0.407
  comQ Acinetobacter baylyi ADP1

41.11

98.322

0.404
  pilQ Legionella pneumophila strain Lp02

38.047

95.944

0.365
  pilQ Legionella pneumophila strain ERS1305867

37.901

95.944

0.364