Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   P5V12_RS00375 Genome accession   NZ_CP120416
Coordinates   77795..79510 (-) Length   571 a.a.
NCBI ID   WP_316955255.1    Uniprot ID   A0AA96KIR0
Organism   Teredinibacter sp. KSP-S5-2     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 72795..84510
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P5V12_RS00345 (P5V12_00345) argJ 73254..74474 (+) 1221 WP_316955249.1 bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ -
  P5V12_RS00350 (P5V12_00350) mutT 74478..74873 (+) 396 WP_316955250.1 8-oxo-dGTP diphosphatase MutT -
  P5V12_RS00355 (P5V12_00355) yacG 74887..75042 (-) 156 WP_410483315.1 DNA gyrase inhibitor YacG -
  P5V12_RS00360 (P5V12_00360) coaE 75086..75703 (-) 618 WP_316955252.1 dephospho-CoA kinase -
  P5V12_RS00365 (P5V12_00365) - 75706..76572 (-) 867 WP_316955253.1 A24 family peptidase -
  P5V12_RS00370 (P5V12_00370) pilC 76578..77789 (-) 1212 WP_316955254.1 type II secretion system F family protein Machinery gene
  P5V12_RS00375 (P5V12_00375) pilB 77795..79510 (-) 1716 WP_316955255.1 type IV-A pilus assembly ATPase PilB Machinery gene
  P5V12_RS00380 (P5V12_00380) - 79631..80362 (-) 732 WP_316955256.1 hypothetical protein -
  P5V12_RS00385 (P5V12_00385) - 80462..81022 (-) 561 WP_316955257.1 pilin -
  P5V12_RS00390 (P5V12_00390) nadC 81186..82037 (-) 852 WP_316955258.1 carboxylating nicotinate-nucleotide diphosphorylase -
  P5V12_RS00395 (P5V12_00395) - 82054..82578 (-) 525 WP_316955259.1 TIGR02281 family clan AA aspartic protease -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 62439.11 Da        Isoelectric Point: 4.7895

>NTDB_id=805513 P5V12_RS00375 WP_316955255.1 77795..79510(-) (pilB) [Teredinibacter sp. KSP-S5-2]
MVLSGLARRLVRDEFLDEDTAHQAISQAEKQKTPLPVFLVENSSISPLDIANAAADEFGTPLIDLSALAKDITPKGLIDP
KLVHKHYAFPLFKRGNRLFVAVSDPTNLHALDEIKFNTGLATEGLLVEADKLQTAIEAYLKAEEESIGDALGNLDEDSLD
DLDIQAVDEDASPSDESSEADEAPIVRFVNKVLLDAIKTGASDIHFEPYEKSYRVRLRTDGILNEVAKPPQNLAGRLAAR
LKVMSQMDISERRVPQDGRIKMKISKTRAIDFRVNSLPTLFGEKIVLRILDPSSAKLGIDALGYEDEQKKLYMDALAQPQ
GMILVTGPTGSGKTVSLYTGLNILNTPERNISTAEDPVEINLEGINQVNVNTKVGLTFAEALRSFLRQDPDIVMVGEIRD
LETAEIGIKAAQTGHLVLSTLHTNSAPETLTRLLNMGVPAFNVATTVSLIIAQRLARRLCSNCKKPADDIPDQVLKEEGF
YEGIGIEKEDFTIFHPVGCNQCNNGYKGRVGVYEVVKITDSISRIIMEGGNAIQIADAARKEGFNNLRTSALRKVATGIT
SLEEANRVTKD

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=805513 P5V12_RS00375 WP_316955255.1 77795..79510(-) (pilB) [Teredinibacter sp. KSP-S5-2]
ATGGTACTTAGCGGATTAGCCCGAAGACTAGTAAGAGACGAGTTCCTTGATGAGGATACAGCCCATCAAGCCATATCCCA
GGCGGAAAAACAAAAAACGCCACTTCCTGTTTTTCTTGTTGAAAATAGCTCAATCAGCCCGTTGGACATTGCCAATGCTG
CTGCGGACGAGTTTGGTACTCCTCTCATAGATCTGTCCGCCCTGGCAAAAGACATTACTCCCAAAGGATTAATTGACCCT
AAACTTGTCCATAAACACTACGCATTCCCTCTTTTCAAAAGAGGTAACCGACTTTTTGTTGCTGTTTCCGACCCCACTAA
CCTTCATGCATTAGATGAGATCAAGTTCAATACAGGCTTGGCAACCGAAGGGCTGTTGGTTGAAGCGGATAAATTGCAAA
CTGCTATTGAAGCTTATCTGAAAGCAGAAGAGGAAAGCATTGGCGATGCGTTAGGAAATCTGGACGAAGATTCTCTGGAC
GACCTCGATATTCAAGCAGTTGACGAGGATGCCTCACCATCAGATGAATCATCGGAAGCCGATGAAGCTCCCATCGTTCG
CTTTGTAAACAAAGTATTACTCGACGCGATAAAAACCGGTGCTTCGGATATTCACTTTGAACCTTACGAAAAATCCTATC
GCGTAAGACTGCGCACAGACGGCATATTGAACGAAGTCGCCAAACCGCCACAAAACCTTGCAGGCAGACTTGCCGCCCGC
CTCAAAGTAATGTCACAAATGGATATTTCAGAGAGACGCGTCCCCCAGGACGGTCGTATTAAGATGAAAATATCCAAAAC
CCGGGCAATCGACTTTCGAGTAAACAGCCTACCAACACTTTTTGGCGAAAAAATTGTACTGCGTATTCTCGACCCATCCA
GTGCCAAGCTGGGTATTGATGCCCTAGGCTACGAAGATGAGCAGAAAAAGCTTTATATGGATGCGCTGGCCCAGCCCCAA
GGCATGATTTTGGTAACCGGGCCGACAGGCTCAGGTAAGACAGTCTCTTTGTACACAGGTTTAAACATACTCAACACGCC
AGAACGGAATATATCCACTGCGGAAGACCCCGTAGAAATCAACCTCGAAGGAATCAACCAGGTTAACGTAAATACCAAGG
TAGGGCTCACCTTTGCAGAGGCTTTACGATCATTTCTGCGACAAGACCCCGACATCGTCATGGTTGGGGAGATCCGTGAC
CTTGAAACAGCCGAGATTGGCATTAAGGCGGCACAAACCGGTCACTTGGTATTATCGACCCTTCATACTAATAGTGCTCC
CGAAACCCTCACCCGCTTGCTTAACATGGGCGTACCGGCTTTTAACGTCGCAACGACAGTAAGCCTGATTATTGCTCAAC
GTCTAGCAAGAAGGCTGTGCAGTAACTGTAAGAAACCTGCAGATGACATACCGGATCAGGTTCTCAAAGAGGAAGGCTTT
TACGAAGGAATTGGGATAGAGAAGGAAGATTTCACCATATTTCACCCTGTGGGCTGCAACCAGTGTAACAATGGCTACAA
AGGGCGGGTCGGGGTTTACGAAGTGGTAAAAATCACAGACTCAATTTCAAGAATAATTATGGAAGGCGGAAACGCTATTC
AGATCGCAGACGCGGCTAGAAAAGAAGGGTTTAATAACCTCCGCACTTCCGCTTTGCGTAAGGTCGCAACGGGCATTACC
AGCCTAGAAGAAGCAAACAGAGTGACTAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(39-145)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

62.456

99.825

0.623
  pilB Acinetobacter baylyi ADP1

61.471

100

0.615
  pilB Legionella pneumophila strain ERS1305867

55.342

100

0.553
  pilB Vibrio cholerae strain A1552

51.408

99.475

0.511
  pilB Vibrio parahaemolyticus RIMD 2210633

51.648

95.622

0.494
  pilF Neisseria gonorrhoeae MS11

48.421

99.825

0.483
  pilB Vibrio campbellii strain DS40M4

55.918

85.814

0.48
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.519

94.571

0.364
  pilB/pilB1 Synechocystis sp. PCC 6803

41.434

87.916

0.364