Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PVE35_RS03735 Genome accession   NZ_CP120359
Coordinates   812089..813792 (-) Length   567 a.a.
NCBI ID   WP_277373844.1    Uniprot ID   -
Organism   Pseudomonas sp. AA-38     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 807089..818792
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PVE35_RS03715 - 807476..808288 (+) 813 WP_277373840.1 YdcF family protein -
  PVE35_RS03720 lnt 808374..809897 (-) 1524 WP_277373841.1 apolipoprotein N-acyltransferase -
  PVE35_RS03725 pilD 809993..810862 (-) 870 WP_277373842.1 A24 family peptidase Machinery gene
  PVE35_RS03730 pilC 810866..812086 (-) 1221 WP_277373843.1 type II secretion system F family protein Machinery gene
  PVE35_RS03735 pilB 812089..813792 (-) 1704 WP_277373844.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PVE35_RS03740 - 814018..814359 (+) 342 WP_277373845.1 pilin -
  PVE35_RS03745 - 814466..815782 (+) 1317 WP_277373846.1 sulfatase-like hydrolase/transferase -
  PVE35_RS03750 - 815764..816714 (+) 951 WP_277373847.1 O-antigen ligase family protein -
  PVE35_RS03755 nadC 817032..817880 (-) 849 WP_277373848.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62676.72 Da        Isoelectric Point: 5.4643

>NTDB_id=805187 PVE35_RS03735 WP_277373844.1 812089..813792(-) (pilB) [Pseudomonas sp. AA-38]
MNDTVSLSGLARQIVAAQLLDEKQAQQAQNQAKRNQLPLVTYLVQNKLVKSRALAELACEQFGVAFIDLHTLDKEGQPKD
LISEKLIRQHRALPLWRRGNKLFIGVSDPTNHQVITDIQFSTGLNTEAILVEDDKLGDAIDKFFESANSGMEDLADVDLD
GLDVEAVDDDKQVGEAGADADDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKTYRVRFRTDGILHEVARPPIQLAPRISAR
LKVMAALDISERRKPQDGRIKMRVSKNKAIDFRVNTLPTLWGEKIVMRILDPTSAQMGIDALGYEEEQKELYMNALKQPQ
GMILVTGPTGSGKTVSLYTGLNILNTPDVNISTAEDPVEINLEGINQVNVNPKQGMDFTQALRAFLRQDPDIIMVGEIRD
LDTASIAVKAAQTGHMVMSTLHTNSAAETLTRLRNMGVPSFNIATSVNLIIAQRLARKLCNQCKKEVDVPREALLAEGFP
EDKIGTFKIYGPVGCENCKGGYKGRVGIYEVVKNTPNLQRIIMEEGNSIDIATQMRKDGFNDLRTSALLKAMQGVTSLEE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=805187 PVE35_RS03735 WP_277373844.1 812089..813792(-) (pilB) [Pseudomonas sp. AA-38]
ATGAACGATACGGTTTCCCTTTCCGGCCTGGCCAGGCAAATCGTGGCGGCACAGCTACTGGATGAAAAACAGGCGCAGCA
AGCTCAAAACCAGGCCAAACGCAATCAGCTACCACTGGTGACCTACCTAGTACAGAACAAGCTGGTCAAAAGCCGCGCCC
TAGCGGAACTGGCCTGCGAGCAATTCGGTGTGGCCTTTATCGACCTGCATACGCTGGACAAGGAGGGCCAACCCAAAGAC
CTGATCAGCGAGAAACTGATTCGCCAGCACCGCGCCCTGCCCCTGTGGCGGCGCGGTAACAAGCTGTTTATCGGCGTTTC
CGACCCCACCAACCACCAGGTGATTACCGATATCCAGTTCAGCACCGGCCTGAATACCGAAGCCATTCTGGTCGAGGATG
ACAAGCTGGGCGATGCCATCGACAAGTTCTTCGAGTCAGCAAACAGTGGCATGGAAGATCTGGCCGATGTCGATCTGGAT
GGTCTGGATGTGGAAGCGGTCGACGATGACAAGCAGGTAGGCGAGGCCGGCGCCGATGCCGATGACGCCCCGGTGGTTCG
CTTCGTCAACAAGATGCTGCTGGATGCCATCAAGGGCGGCTCTTCCGACCTGCACTTCGAGCCCTATGAGAAGACCTATC
GCGTGCGCTTTCGCACCGACGGCATCCTGCATGAAGTAGCGCGCCCGCCAATCCAGTTGGCACCACGCATTTCCGCGCGC
CTCAAGGTGATGGCGGCGCTGGATATCTCCGAGCGGCGCAAGCCGCAGGATGGCCGGATCAAGATGCGTGTATCGAAGAA
CAAGGCCATCGACTTTCGCGTCAATACACTGCCCACCCTGTGGGGCGAGAAGATCGTGATGCGGATTCTCGACCCCACCA
GCGCACAGATGGGTATCGATGCCCTCGGCTACGAAGAAGAGCAGAAAGAACTCTATATGAACGCCCTCAAACAACCGCAG
GGAATGATTCTGGTCACCGGGCCCACCGGCTCGGGCAAGACGGTATCGCTCTATACCGGCCTGAACATCCTAAATACCCC
GGACGTGAACATTTCCACCGCAGAAGATCCGGTGGAGATCAACCTTGAAGGTATCAACCAAGTCAACGTCAACCCGAAGC
AGGGCATGGACTTCACCCAGGCGCTACGCGCCTTCCTACGCCAGGATCCGGATATCATCATGGTGGGTGAGATTCGCGAC
CTGGATACCGCCTCCATCGCCGTCAAGGCCGCGCAGACCGGGCATATGGTGATGTCCACCCTGCACACCAACAGTGCCGC
AGAAACCCTTACACGCCTGCGCAATATGGGTGTGCCCTCCTTCAATATTGCCACCTCGGTGAACCTTATCATCGCCCAGC
GCCTGGCGCGCAAGCTGTGCAACCAATGCAAGAAGGAAGTGGACGTACCACGCGAAGCGCTGCTGGCTGAAGGCTTCCCC
GAAGACAAGATCGGCACCTTCAAGATCTACGGCCCGGTTGGCTGCGAAAATTGCAAGGGCGGTTACAAGGGCCGTGTCGG
TATTTATGAAGTGGTTAAAAACACGCCCAACCTGCAGCGCATTATCATGGAGGAAGGCAACTCCATCGATATCGCCACAC
AGATGCGTAAAGACGGCTTCAATGATCTGCGCACCTCCGCTTTGCTCAAGGCCATGCAAGGGGTTACCAGCCTTGAGGAA
GTCAACCGCGTAACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-147)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.07

100

0.584
  pilB Acinetobacter baylyi ADP1

58.437

99.295

0.58
  pilB Legionella pneumophila strain ERS1305867

54.69

99.647

0.545
  pilB Vibrio cholerae strain A1552

50.089

99.295

0.497
  pilF Neisseria gonorrhoeae MS11

49.204

99.647

0.49
  pilB Vibrio parahaemolyticus RIMD 2210633

49.283

98.413

0.485
  pilB Vibrio campbellii strain DS40M4

47.544

100

0.478
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.752

93.298

0.362