Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   RGU78_RS15060 Genome accession   NZ_CP133712
Coordinates   3135058..3136548 (-) Length   496 a.a.
NCBI ID   WP_001166317.1    Uniprot ID   -
Organism   Acinetobacter baumannii strain CRAb1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3130058..3141548
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RGU78_RS15030 (RGU78_15055) gigA 3131052..3132020 (+) 969 WP_000273590.1 RsbU family protein phosphatase GigA -
  RGU78_RS15035 (RGU78_15060) gigB 3132123..3132644 (+) 522 WP_000103065.1 anti-anti-sigma factor GigB -
  RGU78_RS15040 (RGU78_15065) - 3132671..3133495 (+) 825 WP_000177091.1 carbon-nitrogen hydrolase family protein -
  RGU78_RS15045 (RGU78_15070) - 3133510..3133887 (+) 378 WP_000266123.1 DUF4951 domain-containing protein -
  RGU78_RS15050 (RGU78_15075) - 3134047..3134484 (+) 438 WP_000371736.1 MarR family transcriptional regulator -
  RGU78_RS15055 (RGU78_15080) - 3134572..3135003 (+) 432 WP_000051124.1 organic hydroperoxide resistance protein -
  RGU78_RS15060 (RGU78_15085) pilB 3135058..3136548 (-) 1491 WP_001166317.1 type II secretion system ATPase GspE Machinery gene
  RGU78_RS15065 (RGU78_15090) - 3136645..3137466 (+) 822 WP_000462729.1 hypothetical protein -
  RGU78_RS15070 (RGU78_15095) - 3137476..3138393 (+) 918 WP_000785635.1 mechanosensitive ion channel family protein -
  RGU78_RS15075 (RGU78_15100) - 3138383..3141190 (-) 2808 WP_000276874.1 DUF2339 domain-containing protein -

Sequence

Protein


Download         Length: 496 a.a.        Molecular weight: 55301.17 Da        Isoelectric Point: 6.2037

>NTDB_id=800353 RGU78_RS15060 WP_001166317.1 3135058..3136548(-) (pilB) [Acinetobacter baumannii strain CRAb1]
MQILKQIQVPYSFAKRHGVLFRYDGDQVFIVRRQNTEKIALQEARRILGKPAHYQLCTEQEFNSLLSTSYAGDTGESQQV
AAGLEDHPDLLSLADQVPETEDLMDQEDDAPIVRLINALLSEAIRVGASDIHIEAFEKKLSVRLRVDGQLREIVQPRREL
APLLVSRIKVMAKLDIAEKRVPQDGRISLRLAGREVDVRVSTLPSSHGERVVMRLLDKQAGRLNMTHLGLMANDYERLTQ
LVHRPHGIILVTGPTGSGKTTTLYAALSDLNDNTRNILTAEDPIEYQLEGIGQTQVNTKVDMTFARALKAMLRQDPDVVM
VGEIRDLETAEIAVQASLTGHLVLSTLHTNTAIGAVTRLKDMGIEPFLLSSSLIGAVAQRLVRTLCPHCMTWREADTFEK
QVFQHISHEPSLKLPEAQGCDQCSHLGFNGRTAIYEIVPIDEPMRRLIHGNAAEFELENHARQYSGSIRDDGLRKVLSGK
TTLEEVLRVTNEASEA

Nucleotide


Download         Length: 1491 bp        

>NTDB_id=800353 RGU78_RS15060 WP_001166317.1 3135058..3136548(-) (pilB) [Acinetobacter baumannii strain CRAb1]
ATGCAAATATTGAAACAAATACAAGTTCCATATAGTTTTGCCAAGCGTCATGGTGTTTTATTTCGTTATGACGGTGATCA
AGTTTTTATTGTTAGACGTCAAAATACAGAAAAAATTGCATTGCAGGAAGCAAGACGAATTTTGGGGAAACCTGCACACT
ATCAATTATGCACTGAACAAGAATTTAATAGTCTATTAAGTACCAGTTATGCAGGGGATACAGGCGAATCTCAACAAGTA
GCTGCGGGCTTAGAAGACCATCCGGATTTATTAAGCCTTGCAGATCAGGTGCCTGAAACAGAAGACTTAATGGATCAAGA
AGATGACGCGCCTATTGTTCGTTTGATCAATGCGCTTCTCTCTGAGGCAATTCGTGTTGGTGCTTCAGATATCCATATTG
AAGCCTTTGAGAAAAAGCTATCAGTTCGTTTACGAGTAGATGGACAATTGCGTGAGATTGTACAACCGCGCCGTGAACTA
GCCCCGTTACTTGTTTCGCGTATTAAAGTTATGGCGAAACTCGATATTGCCGAAAAGCGTGTACCGCAAGATGGGCGTAT
TTCTTTACGTCTTGCTGGTCGTGAAGTCGATGTCCGTGTTTCAACTTTGCCTTCTTCTCATGGTGAAAGGGTGGTCATGC
GTTTACTCGATAAACAAGCGGGTCGCCTTAATATGACTCACCTAGGTTTAATGGCAAATGACTATGAACGTTTAACTCAG
CTAGTACATCGTCCGCACGGTATTATTTTGGTCACAGGGCCTACTGGTTCGGGGAAAACGACGACTTTATATGCAGCCTT
ATCTGATCTAAATGATAATACCCGTAACATCTTAACTGCCGAAGATCCAATTGAGTATCAGCTTGAAGGTATTGGCCAAA
CTCAAGTGAATACCAAAGTAGATATGACTTTTGCTCGAGCTTTAAAAGCAATGTTACGTCAAGATCCAGATGTGGTCATG
GTGGGGGAGATCCGTGATCTAGAAACTGCTGAAATTGCGGTGCAGGCATCTTTAACAGGTCATTTGGTGTTATCAACACT
ACATACCAATACCGCTATTGGTGCAGTGACTCGACTTAAAGATATGGGGATTGAACCTTTCCTGCTTTCAAGCTCTTTAA
TTGGGGCGGTTGCGCAGCGTTTGGTTCGTACTTTATGTCCACATTGTATGACATGGCGTGAAGCCGATACTTTTGAGAAG
CAAGTTTTTCAGCATATTTCTCATGAACCAAGTTTAAAGTTACCAGAGGCTCAAGGGTGTGATCAGTGTTCTCATTTAGG
GTTTAATGGCCGTACAGCGATTTATGAAATTGTACCTATTGATGAACCTATGCGCCGTCTCATTCATGGTAATGCAGCTG
AATTTGAATTAGAAAACCATGCTCGTCAGTATTCTGGTTCTATTCGCGATGATGGTTTACGTAAGGTGCTCTCAGGTAAA
ACTACACTTGAAGAAGTGCTTCGTGTAACCAATGAAGCAAGTGAAGCATAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(118-384)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

41.598

98.387

0.409
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.854

99.194

0.405
  pilB Vibrio campbellii strain DS40M4

48.371

80.444

0.389
  pilF Thermus thermophilus HB27

39.148

99.395

0.389
  pilB Vibrio cholerae strain A1552

48

80.645

0.387
  pilB Vibrio parahaemolyticus RIMD 2210633

48

80.645

0.387
  pilB/pilB1 Synechocystis sp. PCC 6803

39.834

97.177

0.387
  pilF Neisseria gonorrhoeae MS11

48.477

79.435

0.385
  pilB Acinetobacter baumannii D1279779

46.898

81.25

0.381
  pilB Acinetobacter baylyi ADP1

46.53

78.427

0.365