Detailed information NTG0800167

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: PX690_RS03860
• Genome accession: NZ_CP119675
• Coordinates: 687715..688329 (-)
• Length: 204 a.a.
• NCBI ID: WP_003152867.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain 160
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 682715..693329

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PX690_RS03830 (PX690_03830)	rpsT	683015..683281 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
PX690_RS03835 (PX690_03835)	holA	683298..684339 (-)	1042	Protein_761	DNA polymerase III subunit delta	-
PX690_RS03840 (PX690_03840)	-	684379..684533 (-)	155	Protein_762	hypothetical protein	-
PX690_RS03845 (PX690_03845)	-	684574..684708 (+)	135	WP_003152870.1	YqzM family protein	-
PX690_RS03850 (PX690_03850)	comEC	684727..687078 (-)	2352	WP_041481887.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
PX690_RS03855 (PX690_03855)	-	687079..687648 (-)	570	WP_003152868.1	ComE operon protein 2	-
PX690_RS03860 (PX690_03860)	comEA	687715..688329 (-)	615	WP_003152867.1	helix-hairpin-helix domain-containing protein	Machinery gene
PX690_RS03865 (PX690_03865)	comER	688388..689209 (+)	822	WP_115996552.1	late competence protein ComER	-
PX690_RS03870 (PX690_03870)	-	689278..690015 (-)	738	WP_014305450.1	class I SAM-dependent methyltransferase	-
PX690_RS03875 (PX690_03875)	rsfS	690012..690368 (-)	357	WP_014305451.1	ribosome silencing factor	-
PX690_RS03880 (PX690_03880)	yqeK	690365..690946 (-)	582	WP_014305452.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
PX690_RS03885 (PX690_03885)	-	690936..691505 (-)	570	WP_014305453.1	nicotinate-nucleotide adenylyltransferase	-
PX690_RS03890 (PX690_03890)	yhbY	691516..691806 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
PX690_RS03895 (PX690_03895)	aroE	691800..692642 (-)	843	WP_003152856.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22269.48 Da
• Isoelectric Point: 6.4940

>NTDB_id=800167 PX690_RS03860 WP_003152867.1 687715..688329(-) (comEA) [Bacillus velezensis strain 160]
MTKAMMTERMRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAVGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMKVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=800167 PX690_RS03860 WP_003152867.1 687715..688329(-) (comEA) [Bacillus velezensis strain 160]
ATGACGAAAGCGATGATGACGGAGCGGATGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCAGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGTCAAACAGCCTCCGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGAGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGTTGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAAGGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	59.375	94.118	0.559
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA	Latilactobacillus sakei subsp. sakei 23K	31.897	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363