Detailed information NTG0799532

Bioinformatically predicted

Overview

• Name: pepF
• Type: Regulator
• Locus tag: P0083_RS12515
• Genome accession: NZ_CP119528
• Coordinates: 2510426..2512237 (-)
• Length: 603 a.a.
• NCBI ID: WP_002387169.1
• Uniprot ID: A0A855UCR5
• Organism: Enterococcus faecalis strain 3143
• Function: degradation of XIP; competence shut-off (predicted from homology) Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
ICE	2503366..2522678	2510426..2512237	within	0

Gene organization within MGE regions

Location: 2503366..2522678

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
P0083_RS12480 (P0083_12480)	cutC	2504312..2504941 (+)	630	WP_002356490.1	copper homeostasis protein CutC	-
P0083_RS12485 (P0083_12485)	mgtE	2505059..2506420 (-)	1362	WP_002362548.1	magnesium transporter	-
P0083_RS12490 (P0083_12490)	-	2506451..2507344 (-)	894	WP_002356487.1	RluA family pseudouridine synthase	-
P0083_RS12495 (P0083_12495)	-	2507352..2508149 (-)	798	WP_002356486.1	NAD kinase	-
P0083_RS12500 (P0083_12500)	-	2508162..2508836 (-)	675	WP_002356484.1	GTP pyrophosphokinase family protein	-
P0083_RS12505 (P0083_12505)	-	2509033..2509611 (+)	579	WP_002356483.1	CYTH domain-containing protein	-
P0083_RS12510 (P0083_12510)	-	2509675..2510334 (+)	660	WP_002368840.1	ClpXP adapter SpxH family protein	-
P0083_RS12515 (P0083_12515)	pepF	2510426..2512237 (-)	1812	WP_002387169.1	oligoendopeptidase F	Regulator
P0083_RS12520 (P0083_12520)	-	2512285..2513463 (-)	1179	WP_002387170.1	competence protein CoiA family protein	-
P0083_RS12525 (P0083_12525)	-	2513553..2514215 (-)	663	WP_002356478.1	adaptor protein MecA	-
P0083_RS12530 (P0083_12530)	spxA	2514557..2514955 (-)	399	WP_002356477.1	transcriptional regulator SpxA	-
P0083_RS12535 (P0083_12535)	dfrF	2515425..2515919 (-)	495	WP_002322211.1	trimethoprim-resistant dihydrofolate reductase DfrF	-
P0083_RS12540 (P0083_12540)	-	2516227..2516706 (-)	480	WP_002322212.1	MGMT family protein	-
P0083_RS12545 (P0083_12545)	-	2516865..2517005 (-)	141	WP_171042677.1	hypothetical protein	-
P0083_RS12550 (P0083_12550)	-	2517017..2517688 (-)	672	WP_002322214.1	helix-turn-helix transcriptional regulator	-
P0083_RS12555 (P0083_12555)	-	2518236..2519246 (+)	1011	WP_009274733.1	replication initiation factor domain-containing protein	-
P0083_RS12560 (P0083_12560)	-	2519246..2519449 (+)	204	WP_002607397.1	excisionase	-
P0083_RS12565 (P0083_12565)	-	2519528..2520721 (+)	1194	WP_010706472.1	site-specific integrase	-
P0083_RS12570 (P0083_12570)	trpS	2521075..2522085 (+)	1011	WP_002378619.1	tryptophan--tRNA ligase	-

Sequence

Protein

• Length: 603 a.a.
• Molecular weight: 68848.16 Da
• Isoelectric Point: 4.6861

>NTDB_id=799532 P0083_RS12515 WP_002387169.1 2510426..2512237(-) (pepF) [Enterococcus faecalis strain 3143]
MSEIKQLPTRDEVPTPLTWDLTKIFKDDAAFDVAYNQLIEELNQAESFKGTLGDGAEAFLAALEYVLDVYRKVETLYVYS
HLKNDQDTTNTAYQALYARASSLYAQVSEAVSWFDPEVLTLSDEQIWGYFEEQPKLAVYRHYIQNILDERPHVLSMEQEA
LLAGASEIFGASSNTFSILNNADLEFPTVQNAEGETIQLSHGVYGQLMESVDPSVREAAFKGLYKVYKQFRNTLASTLGA
HVKTHNYKAKIRNYDSARAASLASNHIPESVHETLVAVVNKHLPLLHRYVKLRKKLLNVEELHMYDLYAPLLGEAPIRYS
YEEAKEKAIEALKPLGEDYLSIVKEAFSSRWIDVIENQGKRSGAYSSGAYDTAPYILMNWHDSLDQLFTLVHEMGHSVHS
YYTRNNQPYVYGDYSIFLAEIASTTNENILTEYLLQTETDPKVRAYVLNHYLDGFKGTIFRQTQFAEFEHFIHTEDAKGT
PLTSEYLSEYYGELNAKYYGPEVVRDEEISYEWARIPHFYYNYYVYQYATGFSAASALSKHILAGEEGALENYLNYLKAG
SSDFPIEVMKKAGVDMTQAAYIEDAMKVFEERLTELEALVEKL

Nucleotide

• Length: 1812 bp

>NTDB_id=799532 P0083_RS12515 WP_002387169.1 2510426..2512237(-) (pepF) [Enterococcus faecalis strain 3143]
ATGAGCGAAATTAAACAATTACCAACACGTGATGAAGTTCCAACTCCATTGACTTGGGATTTAACCAAAATTTTTAAAGA
TGATGCTGCTTTTGATGTTGCGTATAATCAGTTGATAGAAGAGCTAAATCAAGCAGAATCATTTAAAGGTACCTTAGGGG
ATGGTGCAGAAGCTTTTCTAGCTGCGTTAGAATACGTTTTAGATGTCTATCGAAAAGTGGAAACATTATATGTCTATTCT
CATTTAAAAAATGATCAAGACACTACAAATACAGCTTACCAAGCTTTATATGCCAGAGCTAGTTCTTTATATGCACAAGT
TAGTGAAGCTGTTTCTTGGTTTGACCCAGAGGTTTTAACATTAAGTGATGAACAAATCTGGGGATATTTTGAAGAGCAGC
CGAAATTAGCTGTTTATCGTCACTATATTCAAAATATTTTAGATGAACGTCCCCATGTTTTATCGATGGAACAAGAAGCT
TTACTGGCGGGGGCTAGTGAAATTTTTGGTGCCTCAAGTAATACATTTTCAATTTTAAATAATGCAGACTTAGAATTTCC
AACTGTGCAAAATGCTGAAGGCGAAACGATTCAACTTTCTCATGGCGTTTATGGTCAGTTAATGGAAAGTGTCGATCCAT
CTGTTCGTGAAGCAGCATTTAAAGGTTTGTACAAAGTATACAAACAATTTAGAAATACATTAGCCTCAACTTTAGGTGCA
CATGTTAAAACACATAATTATAAAGCAAAAATTAGAAATTATGATTCTGCTCGAGCAGCCTCTTTAGCAAGTAATCATAT
CCCTGAAAGTGTTCACGAAACATTAGTAGCTGTAGTAAATAAACATTTACCTTTGCTACATCGTTATGTAAAATTAAGAA
AAAAATTATTAAACGTAGAAGAATTACACATGTACGACTTGTATGCGCCTTTGCTAGGTGAAGCACCAATTCGTTACAGC
TATGAAGAAGCAAAAGAAAAAGCAATTGAGGCTTTAAAACCACTAGGTGAAGACTATTTATCTATTGTTAAAGAAGCTTT
TTCAAGTCGCTGGATTGATGTGATTGAAAATCAAGGAAAACGAAGCGGCGCATATTCTTCAGGAGCTTATGATACAGCCC
CATACATTTTAATGAATTGGCATGATAGTTTGGATCAACTATTTACATTAGTCCATGAGATGGGCCATAGTGTTCATAGT
TACTATACAAGAAATAATCAGCCGTATGTTTATGGCGACTATTCAATTTTCTTAGCTGAGATTGCTTCAACCACAAATGA
AAATATTTTAACAGAATATTTATTACAAACAGAAACAGATCCTAAAGTACGTGCGTATGTCTTAAATCACTATTTAGACG
GCTTTAAGGGAACCATTTTCCGTCAAACGCAATTTGCGGAATTTGAACATTTTATTCACACGGAAGATGCTAAAGGCACG
CCATTAACAAGTGAATATTTGAGTGAGTATTATGGCGAGCTCAATGCTAAATATTATGGACCAGAAGTAGTTAGAGACGA
AGAAATCAGTTACGAATGGGCGAGAATTCCACATTTTTATTACAATTACTATGTTTATCAATATGCAACTGGCTTCTCAG
CCGCTTCGGCATTGTCTAAACATATTTTAGCTGGAGAAGAGGGAGCTTTAGAGAATTATCTTAACTACTTGAAAGCAGGA
AGTAGTGACTTCCCAATTGAAGTGATGAAAAAAGCGGGCGTGGATATGACACAAGCCGCGTATATCGAAGATGCAATGAA
AGTTTTTGAAGAACGTTTAACGGAATTAGAAGCTTTGGTTGAAAAATTATAG

Domains

Predicted by InterproScan.

• Peptidase family M3 (Peptidase_M3) (207-587)

• Oligopeptidase F (Peptidase_M3_N) (117-186)

3D structure

• AlphaFold DB: A0A855UCR5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pepF	Streptococcus salivarius strain HSISS4	54.806	98.342	0.539