Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   P0M04_RS03200 Genome accession   NZ_CP119520
Coordinates   731589..733322 (+) Length   577 a.a.
NCBI ID   WP_259448789.1    Uniprot ID   -
Organism   Telluria mixta strain ACM 1762     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 726589..738322
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P0M04_RS03180 (P0M04_03180) - 727000..727401 (+) 402 WP_259448792.1 hypothetical protein -
  P0M04_RS03185 (P0M04_03185) - 727470..729041 (+) 1572 WP_281042368.1 methyl-accepting chemotaxis protein -
  P0M04_RS03190 (P0M04_03190) - 729228..729911 (+) 684 WP_259448791.1 hypothetical protein -
  P0M04_RS03195 (P0M04_03195) - 730035..731342 (+) 1308 WP_259448790.1 HlyC/CorC family transporter -
  P0M04_RS03200 (P0M04_03200) pilB 731589..733322 (+) 1734 WP_259448789.1 type IV-A pilus assembly ATPase PilB Machinery gene
  P0M04_RS03205 (P0M04_03205) pilC 733332..734570 (+) 1239 WP_259448788.1 type II secretion system F family protein Machinery gene
  P0M04_RS03210 (P0M04_03210) - 735123..735407 (+) 285 WP_259448787.1 hypothetical protein -
  P0M04_RS03215 (P0M04_03215) - 735478..736350 (+) 873 WP_259448786.1 hypothetical protein -
  P0M04_RS03220 (P0M04_03220) - 736457..737644 (-) 1188 WP_259448785.1 phospholipase D-like domain-containing protein -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 61883.26 Da        Isoelectric Point: 5.0856

>NTDB_id=799410 P0M04_RS03200 WP_259448789.1 731589..733322(+) (pilB) [Telluria mixta strain ACM 1762]
MAAVLPNTTPGTALPGLARALVQAGRLTLPQADTLQKKASADKAGFIDTLLGSSSIDAKSLAAFCAETFGYPLLDLGTFS
PDALPVNAIDPKLMQAQRVVALARRGNKLSVALSDPTNTQALDQIKFQSESTVEPVIVAHDTLLALLATIAKSAEQDLSE
LAGEEGDIEFAEDEPQTQAAEVAAAAAEVEDAPIVRFLNKVLMDAITLGASDIHFEPYEKQYRIRLRVDGVLRDHLAPPL
SIRDKLVSRIKVLAKLDISEKRVPQDGRMRLVLSPTKTIDFRVSTLPTLFGEKTVMRILDATQAQMGIDSLGYDPDQKAL
LIDAITRPYGMVLVTGPTGSGKTVSLYSCLNLLNKPGINISTAEDPAEINLPGVNQVNVNEKAGLTFPIALKAFLRQDPD
IIMVGEIRDLETADIAIKAAQTGHMVFSTLHTNDAPSTLTRLMNMGVAPFNIASSVILITAQRLARRLCTCKQPLDIGRD
TLKAAGFRDTDFDGDWKPYGPVGCERCLGSGYKGRVGIYQIMPITPAIEALILGHGNAMEIAAQAEAEGVNSLRRSGLLK
VKQGLTSLEEVLGCTNE

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=799410 P0M04_RS03200 WP_259448789.1 731589..733322(+) (pilB) [Telluria mixta strain ACM 1762]
ATGGCAGCAGTCCTTCCCAACACCACCCCTGGCACTGCGCTGCCCGGGCTGGCGCGTGCCCTGGTGCAGGCCGGACGGCT
GACCTTGCCTCAAGCGGACACCTTGCAAAAGAAGGCGTCCGCCGACAAGGCCGGTTTCATCGACACCTTGCTCGGCAGCA
GCTCCATCGATGCAAAGTCCCTGGCAGCCTTCTGCGCCGAGACGTTCGGCTACCCCCTGCTCGACCTCGGCACGTTCAGC
CCCGACGCCCTGCCCGTCAACGCGATCGACCCGAAGCTGATGCAGGCCCAGCGCGTCGTCGCGCTGGCACGCAGGGGCAA
CAAGTTGTCGGTGGCACTGTCCGACCCGACGAATACGCAGGCGCTCGACCAGATCAAGTTCCAGAGCGAGTCGACCGTCG
AGCCGGTCATCGTCGCGCACGACACCCTGCTGGCACTGCTGGCGACCATCGCCAAGAGCGCCGAACAGGACTTGAGCGAG
CTGGCCGGCGAGGAAGGCGACATCGAATTCGCCGAGGACGAACCGCAGACGCAGGCGGCGGAAGTGGCCGCGGCTGCGGC
GGAAGTCGAGGACGCGCCGATCGTCCGCTTCCTGAACAAGGTCCTGATGGATGCAATTACCTTGGGCGCGTCGGACATCC
ACTTCGAGCCCTACGAAAAGCAGTACCGTATCCGCCTGCGCGTGGACGGCGTGCTGCGCGACCATCTGGCGCCGCCGCTG
TCGATCCGCGACAAGCTCGTCTCGCGCATCAAGGTGCTCGCCAAGCTCGACATCTCGGAAAAGCGCGTGCCGCAGGACGG
CCGCATGCGCCTCGTGCTGTCGCCCACGAAGACGATCGATTTCCGCGTGAGCACCTTGCCGACCTTGTTCGGCGAAAAGA
CGGTCATGCGTATCCTCGACGCGACACAGGCCCAGATGGGCATCGACTCGCTCGGCTACGATCCCGACCAGAAGGCCCTC
TTGATCGACGCCATCACGCGGCCGTACGGCATGGTGCTCGTGACGGGCCCGACGGGTTCCGGTAAAACCGTGTCGCTGTA
CAGCTGCCTGAACCTGTTGAACAAGCCCGGGATCAATATCTCCACAGCGGAAGACCCGGCCGAGATCAACCTGCCCGGCG
TGAACCAGGTCAACGTCAACGAAAAAGCGGGCCTCACGTTCCCCATCGCGCTGAAAGCCTTCCTGCGCCAGGACCCGGAC
ATCATCATGGTCGGCGAGATCCGCGACCTGGAAACGGCCGACATCGCCATCAAGGCGGCGCAGACGGGCCACATGGTGTT
TTCCACCCTGCACACGAACGATGCGCCGTCGACCCTGACGCGTTTGATGAACATGGGCGTGGCGCCGTTCAACATCGCCT
CGTCCGTCATTTTGATTACCGCGCAACGTCTCGCGCGCCGCCTGTGCACGTGCAAGCAACCGCTCGATATCGGCCGCGAC
ACGCTGAAGGCCGCGGGCTTCCGCGACACCGATTTCGACGGCGACTGGAAACCGTACGGCCCCGTCGGCTGCGAGCGCTG
CCTCGGCTCCGGCTACAAGGGCCGCGTGGGCATCTACCAGATCATGCCGATCACGCCCGCGATCGAAGCGCTGATCCTCG
GGCACGGCAACGCGATGGAGATCGCCGCCCAGGCCGAGGCCGAGGGCGTGAACTCGCTGCGCCGTTCCGGCCTGCTGAAA
GTGAAACAAGGCCTCACCAGTCTCGAAGAAGTGCTCGGCTGTACGAACGAATAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(200-467)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(56-154)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

54.895

99.133

0.544
  pilB Acinetobacter baumannii D1279779

54.34

99.827

0.542
  pilF Neisseria gonorrhoeae MS11

53.982

97.92

0.529
  pilB Legionella pneumophila strain ERS1305867

49.02

97.227

0.477
  pilB Vibrio campbellii strain DS40M4

45.739

99.653

0.456
  pilB Vibrio cholerae strain A1552

45.503

98.267

0.447
  pilB Vibrio parahaemolyticus RIMD 2210633

45.745

97.747

0.447
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.572

97.227

0.385
  pilF Thermus thermophilus HB27

40.485

92.894

0.376