Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PY254_RS06570 Genome accession   NZ_CP119392
Coordinates   1545549..1547273 (-) Length   574 a.a.
NCBI ID   WP_281014676.1    Uniprot ID   -
Organism   Rhodanobacter sp. AS-Z3     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1540549..1552273
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PY254_RS06545 (PY254_06545) - 1541008..1541496 (+) 489 WP_281014671.1 dihydrofolate reductase -
  PY254_RS06550 (PY254_06550) - 1541526..1542461 (+) 936 WP_281014672.1 Nudix family hydrolase -
  PY254_RS06555 (PY254_06555) coaE 1542523..1543143 (-) 621 WP_281014673.1 dephospho-CoA kinase -
  PY254_RS06560 (PY254_06560) pilD 1543140..1543997 (-) 858 WP_281014674.1 A24 family peptidase Machinery gene
  PY254_RS06565 (PY254_06565) pilC 1544135..1545406 (-) 1272 WP_281014675.1 type II secretion system F family protein Machinery gene
  PY254_RS06570 (PY254_06570) pilB 1545549..1547273 (-) 1725 WP_281014676.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PY254_RS06575 (PY254_06575) - 1547703..1548557 (+) 855 WP_281014677.1 glycosyltransferase family 2 protein -
  PY254_RS06580 (PY254_06580) - 1548550..1549710 (+) 1161 WP_281014678.1 glycosyltransferase family 4 protein -
  PY254_RS06585 (PY254_06585) - 1549703..1550473 (+) 771 WP_281014679.1 class I SAM-dependent methyltransferase -
  PY254_RS06590 (PY254_06590) - 1550520..1551569 (-) 1050 WP_281014680.1 glycosyltransferase family 4 protein -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 62522.08 Da        Isoelectric Point: 5.7843

>NTDB_id=798792 PY254_RS06570 WP_281014676.1 1545549..1547273(-) (pilB) [Rhodanobacter sp. AS-Z3]
MSSQSQPSLTGLSGIARRLVSDGIMAEEVVRQAMLDSSQKKASLGTWLLDKNLVDGAELTRVSSIEFGMPMMDVASLNPA
TMPLDLISEALITKHQALPLFRRGKRLYVGIADPMQSHALDEIKFNSNCMVEPILVERASLQKVIEGLLNNMRNTMPDMG
GGDFDELDIEAGDDEAESSSGVDATGTDDAPVVKFVNKVLVDAIRRGASDIHFEPFETQYRVRVRLDGILSTVANPPMKM
ANRISSRIKVMSQLDIAEKRVPQDGRIKLNLSKTRAVDFRVSVLPTLFGEKIVMRILDGSSAKIGIEKLGYEPDQQKLYV
DAIHKPYGLVLVTGPTGSGKTVSLYTGLNMLNTPERNISTVEDPVEIRVEGINQVQQNVKRGMTFASALRSFLRQDPDVI
MVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAAQTVSRLMNMGIAPYNITSSVSLIIAQRLARRLHDCKKVSDLPPQVL
LAAGFSQADVDAGMTVYEAVGCDGCNDGYKGRVGIYQVMPMLEEIQRIILEGGNTMQIAAAAKAAGVNDLRASALLKVKQ
GLTSLIEIDRVTKE

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=798792 PY254_RS06570 WP_281014676.1 1545549..1547273(-) (pilB) [Rhodanobacter sp. AS-Z3]
ATGTCATCTCAATCGCAACCATCGCTCACGGGACTGTCCGGCATAGCGCGCCGATTGGTGTCTGATGGAATCATGGCCGA
AGAAGTGGTCCGCCAGGCCATGCTGGACAGCAGTCAGAAAAAGGCTTCGTTAGGCACCTGGTTGCTGGACAAAAACCTGG
TCGACGGCGCCGAACTGACGCGGGTTTCTTCCATCGAATTCGGCATGCCGATGATGGACGTGGCATCACTGAACCCGGCC
ACGATGCCGTTGGACCTGATCAGTGAAGCGCTGATCACCAAGCATCAGGCGTTGCCCTTGTTCCGCCGCGGCAAGCGCTT
GTATGTGGGCATTGCCGACCCGATGCAGTCGCACGCGCTGGACGAGATCAAGTTCAACTCCAACTGCATGGTCGAGCCCA
TCCTGGTGGAGCGCGCCTCGCTGCAGAAGGTGATCGAAGGCCTGCTCAACAATATGCGCAACACCATGCCGGACATGGGT
GGTGGCGACTTCGACGAGCTCGATATCGAAGCGGGCGATGACGAGGCCGAGTCGAGCAGCGGCGTCGATGCCACCGGAAC
CGACGACGCGCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTCGACGCGATCCGTCGCGGTGCCTCGGATATTCACTTCG
AGCCGTTTGAGACCCAGTACCGCGTGCGTGTACGCCTGGATGGCATCCTCAGCACGGTGGCCAACCCACCGATGAAGATG
GCCAACCGCATCTCCTCGCGCATCAAGGTGATGTCGCAGTTGGACATCGCCGAGAAGCGCGTGCCGCAGGATGGCCGCAT
CAAGCTCAACCTGTCCAAGACCCGCGCGGTCGACTTCCGCGTCAGCGTGCTGCCGACGCTGTTCGGCGAAAAGATCGTGA
TGCGTATTCTGGACGGCTCCTCGGCCAAGATCGGCATCGAGAAGCTGGGCTACGAGCCGGACCAGCAGAAGCTCTATGTG
GACGCGATCCACAAGCCCTACGGCCTGGTGCTGGTCACCGGCCCGACCGGCTCCGGCAAAACGGTGTCGCTGTACACCGG
CCTCAACATGCTCAACACGCCGGAGCGCAACATCTCCACGGTGGAGGACCCGGTCGAAATTCGCGTCGAAGGGATCAATC
AGGTCCAGCAGAACGTCAAGCGCGGCATGACCTTCGCCAGCGCACTGCGCTCGTTCCTGCGCCAGGATCCGGACGTGATC
ATGGTCGGCGAAATCCGCGATCTGGAAACCGCCGAGATTGCCATCAAGGCCGCGCAAACCGGCCACATGGTGCTGTCCAC
CCTGCACACCAACGACGCGGCGCAGACGGTTTCGCGCCTGATGAACATGGGCATCGCGCCCTACAACATCACCTCGTCGG
TCAGCCTGATCATTGCCCAGCGTCTGGCGCGACGCCTGCACGACTGCAAAAAAGTGTCGGACCTGCCGCCGCAAGTGCTG
TTGGCGGCCGGCTTCAGCCAGGCCGACGTCGATGCCGGCATGACCGTCTACGAGGCGGTCGGCTGCGACGGCTGCAACGA
TGGCTACAAGGGCCGCGTGGGCATTTATCAGGTCATGCCGATGCTGGAAGAAATCCAGAGAATCATCCTGGAAGGCGGCA
ACACCATGCAGATTGCCGCAGCGGCCAAGGCTGCCGGGGTGAATGACTTGCGCGCCTCAGCCTTGCTGAAGGTCAAGCAA
GGTCTGACCAGCCTGATCGAGATTGACCGTGTCACCAAGGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(198-465)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(48-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

54.035

99.303

0.537
  pilB Acinetobacter baylyi ADP1

53.125

100

0.533
  pilB Legionella pneumophila strain ERS1305867

50.352

98.955

0.498
  pilB Vibrio cholerae strain A1552

49.016

97.387

0.477
  pilB Vibrio campbellii strain DS40M4

47.321

97.561

0.462
  pilF Neisseria gonorrhoeae MS11

46.691

97.387

0.455
  pilB Vibrio parahaemolyticus RIMD 2210633

48.134

93.38

0.449
  pilF Thermus thermophilus HB27

37.882

97.038

0.368
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.574

95.296

0.368