Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYB   Type   Machinery gene
Locus tag   PY824_RS00815 Genome accession   NZ_CP119172
Coordinates   117542..118627 (+) Length   361 a.a.
NCBI ID   WP_014293764.1    Uniprot ID   A0AA47FE98
Organism   Streptococcus macedonicus strain SGM CIP105683     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 112542..123627
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PY824_RS00805 (PY824_00805) - 116280..116609 (+) 330 WP_014293762.1 DUF1033 family protein -
  PY824_RS00810 (PY824_00810) comYA 116719..117660 (+) 942 WP_014293763.1 competence type IV pilus ATPase ComGA Machinery gene
  PY824_RS00815 (PY824_00815) comYB 117542..118627 (+) 1086 WP_014293764.1 competence type IV pilus assembly protein ComGB Machinery gene
  PY824_RS00820 (PY824_00820) comYC 118627..118920 (+) 294 WP_039670352.1 competence type IV pilus major pilin ComGC Machinery gene
  PY824_RS00825 (PY824_00825) comYD 118904..119335 (+) 432 WP_014293766.1 competence type IV pilus minor pilin ComGD Machinery gene
  PY824_RS00830 (PY824_00830) comGE 119289..119582 (+) 294 WP_044562943.1 competence type IV pilus minor pilin ComGE -
  PY824_RS00835 (PY824_00835) comYF 119536..120003 (+) 468 WP_014293768.1 competence type IV pilus minor pilin ComGF Machinery gene
  PY824_RS00840 (PY824_00840) comYG 119975..120280 (+) 306 WP_014293769.1 competence type IV pilus minor pilin ComGG Machinery gene
  PY824_RS00845 (PY824_00845) comYH 120362..121318 (+) 957 WP_014293770.1 class I SAM-dependent methyltransferase Machinery gene
  PY824_RS00850 (PY824_00850) - 121371..122570 (+) 1200 WP_014293771.1 acetate kinase -
  PY824_RS00855 (PY824_00855) - 122732..122932 (+) 201 WP_012961332.1 helix-turn-helix transcriptional regulator -
  PY824_RS00860 (PY824_00860) - 122942..123151 (+) 210 WP_014293772.1 hypothetical protein -
  PY824_RS00865 (PY824_00865) - 123164..123616 (+) 453 WP_014293773.1 hypothetical protein -

Sequence

Protein


Download         Length: 361 a.a.        Molecular weight: 41359.34 Da        Isoelectric Point: 10.1651

>NTDB_id=798020 PY824_RS00815 WP_014293764.1 117542..118627(+) (comYB) [Streptococcus macedonicus strain SGM CIP105683]
MLKKTLKVTNQSAGIQKLKVLLKTDISQLNKQKSKKLPFKKQRKVIQLFNNLFESGFNLTEIVSFLRRSQLLLDIYVERM
QESLLNGARLATMMADLGFSDSIVTQIALADVHGNSQKSLLKIESYLSSMTVVRKKLIEVATYPLILLLFLILIMLGMKN
YLLPQLESQNVATQIITHFPTIFLLSIFSIGVLLVCATFYARRLSQIDLYSRISRIPLVGNYVRLYLTAYYAREWGNLIG
QGIELMAIVKIMQKQKPLLFQEIGKDMEEALLSGQAFHQKVLGYPFFLRELSLMIEYGEVKSKLGRELDIYAEETWQRFF
GKLTQATQLIQPLVFVFVALIIVLIYVAMLLPMYQNMGGNF

Nucleotide


Download         Length: 1086 bp        

>NTDB_id=798020 PY824_RS00815 WP_014293764.1 117542..118627(+) (comYB) [Streptococcus macedonicus strain SGM CIP105683]
TTGCTAAAGAAAACTTTGAAAGTCACCAACCAGAGTGCTGGAATACAAAAATTGAAAGTCTTGTTAAAGACGGACATATC
ACAGCTGAACAAGCAAAAATCGAAAAAATTGCCATTTAAAAAACAGCGCAAGGTTATTCAACTCTTTAATAATCTTTTTG
AAAGTGGGTTTAATTTAACAGAAATCGTGTCTTTTCTCCGAAGAAGTCAATTGTTGTTAGACATTTATGTTGAGAGGATG
CAAGAAAGTTTGTTAAATGGTGCTAGACTAGCAACAATGATGGCAGATTTAGGGTTTTCAGACAGTATTGTCACACAAAT
TGCTTTGGCTGATGTTCATGGAAACAGTCAGAAAAGTCTGCTGAAGATTGAGTCTTACCTTTCTAGCATGACTGTCGTCA
GAAAAAAGTTGATTGAAGTTGCAACGTATCCATTGATTTTGTTGTTGTTTCTTATTTTGATTATGCTGGGGATGAAGAAT
TATCTACTGCCACAGCTGGAAAGCCAAAATGTGGCAACGCAGATTATTACGCATTTTCCAACGATTTTTTTGCTAAGTAT
TTTCTCGATTGGAGTCTTGCTTGTTTGTGCGACATTTTATGCTAGGCGTTTATCGCAGATTGATTTATATAGTCGCATAA
GCCGGATTCCACTTGTGGGAAACTATGTTAGGTTATATTTGACAGCTTACTATGCGCGTGAATGGGGAAATTTGATTGGG
CAAGGTATTGAATTAATGGCAATCGTGAAAATCATGCAAAAGCAAAAGCCGCTCTTATTTCAAGAGATTGGAAAGGATAT
GGAAGAAGCGTTGCTTTCAGGGCAAGCTTTTCATCAAAAAGTTCTAGGCTATCCATTCTTTTTGCGAGAATTGAGCCTAA
TGATTGAATATGGTGAGGTCAAATCGAAGCTGGGACGTGAGTTAGACATTTATGCTGAGGAAACATGGCAGAGATTCTTT
GGCAAATTGACTCAAGCAACACAGCTCATTCAACCACTTGTTTTTGTTTTTGTGGCTTTGATTATTGTGTTAATTTATGT
GGCAATGTTGTTGCCAATGTATCAAAATATGGGAGGAAATTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(232-352)

Type II secretion system (T2SS), protein F (T2SSF)

(47-164)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYB Streptococcus mutans UA140

66.139

87.535

0.579
  comYB Streptococcus mutans UA159

65.823

87.535

0.576
  comGB/cglB Streptococcus mitis NCTC 12261

59.587

93.906

0.56
  comYB Streptococcus gordonii str. Challis substr. CH1

59.821

93.075

0.557
  comGB/cglB Streptococcus mitis SK321

56.973

93.352

0.532
  comGB/cglB Streptococcus pneumoniae Rx1

56.083

93.352

0.524
  comGB/cglB Streptococcus pneumoniae D39

56.083

93.352

0.524
  comGB/cglB Streptococcus pneumoniae R6

56.083

93.352

0.524
  comGB/cglB Streptococcus pneumoniae TIGR4

56.083

93.352

0.524
  comGB Lactococcus lactis subsp. cremoris KW2

49.123

94.737

0.465