Detailed information NTG0798023

Bioinformatically predicted

Overview

• Name: comYF
• Type: Machinery gene
• Locus tag: PY824_RS00835
• Genome accession: NZ_CP119172
• Coordinates: 119536..120003 (+)
• Length: 155 a.a.
• NCBI ID: WP_014293768.1
• Uniprot ID: A0A380K3J6
• Organism: Streptococcus macedonicus strain SGM CIP105683
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 114536..125003

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PY824_RS00805 (PY824_00805)	-	116280..116609 (+)	330	WP_014293762.1	DUF1033 family protein	-
PY824_RS00810 (PY824_00810)	comYA	116719..117660 (+)	942	WP_014293763.1	competence type IV pilus ATPase ComGA	Machinery gene
PY824_RS00815 (PY824_00815)	comYB	117542..118627 (+)	1086	WP_014293764.1	competence type IV pilus assembly protein ComGB	Machinery gene
PY824_RS00820 (PY824_00820)	comYC	118627..118920 (+)	294	WP_039670352.1	competence type IV pilus major pilin ComGC	Machinery gene
PY824_RS00825 (PY824_00825)	comYD	118904..119335 (+)	432	WP_014293766.1	competence type IV pilus minor pilin ComGD	Machinery gene
PY824_RS00830 (PY824_00830)	comGE	119289..119582 (+)	294	WP_044562943.1	competence type IV pilus minor pilin ComGE	-
PY824_RS00835 (PY824_00835)	comYF	119536..120003 (+)	468	WP_014293768.1	competence type IV pilus minor pilin ComGF	Machinery gene
PY824_RS00840 (PY824_00840)	comYG	119975..120280 (+)	306	WP_014293769.1	competence type IV pilus minor pilin ComGG	Machinery gene
PY824_RS00845 (PY824_00845)	comYH	120362..121318 (+)	957	WP_014293770.1	class I SAM-dependent methyltransferase	Machinery gene
PY824_RS00850 (PY824_00850)	-	121371..122570 (+)	1200	WP_014293771.1	acetate kinase	-
PY824_RS00855 (PY824_00855)	-	122732..122932 (+)	201	WP_012961332.1	helix-turn-helix transcriptional regulator	-
PY824_RS00860 (PY824_00860)	-	122942..123151 (+)	210	WP_014293772.1	hypothetical protein	-
PY824_RS00865 (PY824_00865)	-	123164..123616 (+)	453	WP_014293773.1	hypothetical protein	-
PY824_RS00870 (PY824_00870)	-	123628..124263 (+)	636	WP_014293774.1	CPBP family intramembrane glutamic endopeptidase	-

Sequence

Protein

• Length: 155 a.a.
• Molecular weight: 17564.08 Da
• Isoelectric Point: 9.2496

>NTDB_id=798023 PY824_RS00835 WP_014293768.1 119536..120003(+) (comYF) [Streptococcus macedonicus strain SGM CIP105683]
MFMRGKRKYYTLKKTSLEAFSLIECLVSLLVISGAILVYNGLTQYISANVHYLSENQEENWLLFSQQLRAELANCQLDKV
ENNKLYVTKSSQKLAFGQSKADDFRKTNASGQGYQPMIFGVKSSAISRDGQKVTMTLNLENGLERTFVYTFETAS

Nucleotide

• Length: 468 bp

>NTDB_id=798023 PY824_RS00835 WP_014293768.1 119536..120003(+) (comYF) [Streptococcus macedonicus strain SGM CIP105683]
ATGTTTATGAGGGGAAAACGGAAATATTACACGTTAAAAAAGACTAGTTTAGAGGCATTCAGCCTCATAGAATGTTTGGT
TTCTTTATTAGTAATTTCGGGTGCTATTCTTGTTTACAATGGCTTAACGCAATATATTTCTGCAAATGTGCATTATTTGT
CGGAAAATCAAGAGGAAAACTGGCTTTTGTTTTCACAACAGCTGCGTGCGGAGCTTGCCAATTGTCAATTAGATAAGGTT
GAAAATAACAAACTATATGTGACGAAGTCCAGTCAAAAGTTGGCATTTGGACAGTCAAAGGCTGATGATTTTCGTAAAAC
AAACGCATCTGGTCAAGGCTATCAGCCAATGATATTTGGAGTAAAATCTTCTGCTATTTCTAGAGATGGTCAGAAGGTGA
CAATGACATTGAATTTAGAGAATGGTTTGGAGAGGACATTTGTTTACACTTTTGAAACGGCAAGTTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (49-144)

3D structure

• AlphaFold DB: A0A380K3J6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comYF	Streptococcus mutans UA140	56.835	89.677	0.51
comYF	Streptococcus mutans UA159	56.115	89.677	0.503
comGF/cglF	Streptococcus pneumoniae Rx1	44.286	90.323	0.4
comGF/cglF	Streptococcus pneumoniae D39	44.286	90.323	0.4
comGF/cglF	Streptococcus pneumoniae R6	44.286	90.323	0.4
comGF/cglF	Streptococcus pneumoniae TIGR4	44.286	90.323	0.4
comGF/cglF	Streptococcus mitis NCTC 12261	42.958	91.613	0.394
comGF/cglF	Streptococcus mitis SK321	42.254	91.613	0.387
comGF	Lactococcus lactis subsp. cremoris KW2	43.478	89.032	0.387