Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PXX05_RS06040 Genome accession   NZ_CP119078
Coordinates   1399889..1401577 (+) Length   562 a.a.
NCBI ID   WP_275090163.1    Uniprot ID   -
Organism   Legionella cardiaca strain H63     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1394889..1406577
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PXX05_RS06025 (PXX05_06025) - 1397501..1398715 (-) 1215 WP_275090160.1 MFS transporter -
  PXX05_RS06030 (PXX05_06030) - 1398717..1399466 (-) 750 WP_275090161.1 methyltransferase domain-containing protein -
  PXX05_RS06035 (PXX05_06035) - 1399549..1399782 (-) 234 WP_275090162.1 hypothetical protein -
  PXX05_RS06040 (PXX05_06040) pilB 1399889..1401577 (+) 1689 WP_275090163.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PXX05_RS06045 (PXX05_06045) pilC 1401585..1402799 (+) 1215 WP_275090164.1 type II secretion system F family protein Machinery gene
  PXX05_RS06050 (PXX05_06050) - 1402805..1403671 (+) 867 WP_275090165.1 prepilin peptidase -
  PXX05_RS06055 (PXX05_06055) - 1403752..1404546 (+) 795 WP_275090166.1 F-box protein -
  PXX05_RS06060 (PXX05_06060) - 1404536..1405435 (-) 900 WP_275090167.1 aminoglycoside phosphotransferase family protein -
  PXX05_RS06065 (PXX05_06065) - 1405540..1406127 (-) 588 WP_275090168.1 hypothetical protein -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 63357.37 Da        Isoelectric Point: 8.0648

>NTDB_id=797541 PXX05_RS06040 WP_275090163.1 1399889..1401577(+) (pilB) [Legionella cardiaca strain H63]
MERIYAEHPLPTIAHLMVKANLISNVKINEYQKNAQSSKQTLLEYLTANKLFSARQLALLLSEHFGVPYLDLDSINLTLM
PWQLLNEKWVYNLRLIPLFIRGNHLFLATDDPSKHATIKEIQFYTQFYPLVIVVERDKLNGLIEKINQHKSNPGSYEIAT
TEKFTERLSDPGFKEDAPIVKLVHDILADAVKRKASDVHFELYEDQYRVRYRLDGVLVEAVAPPTSLANQITSRIKILAN
LDISERRIPQDGRFRMEMAERFTMDFRVNVCPTVSGEKIAIRILNPNISTVSIEELGFNSFQQQLFLRSLEKPQGMILIT
GPTGSGKTASLYTALTLLNTPERNILTAEDPVEIKISGINQININPKVGLTFANALRSFLRQDPDIIMVGEIRDLETAEI
AIKASHTGHLVLSTLHTNSAAEALTRLRNMGVPSFNIASSLNLLIAQRLVRRLCSYCKKVTKILTASELIQLGFEKEIIN
PVELYSADGCKHCLNGYKGRIGLFEILPVSPTIADLILSNVNSIDILRQAKQEGMLTLRQTGFEKVRDGITTIEELNRVT
IN

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=797541 PXX05_RS06040 WP_275090163.1 1399889..1401577(+) (pilB) [Legionella cardiaca strain H63]
ATGGAAAGAATTTACGCAGAACACCCATTACCAACCATTGCTCATTTAATGGTTAAAGCCAATCTAATCAGCAACGTTAA
AATAAATGAGTACCAAAAAAATGCTCAATCTTCTAAGCAAACATTATTAGAATACCTAACTGCAAATAAACTTTTTTCGG
CGCGGCAATTAGCATTGTTATTATCGGAGCATTTTGGTGTTCCTTACCTTGATCTTGATTCGATTAATTTAACCTTAATG
CCTTGGCAATTATTGAATGAAAAATGGGTATACAATCTTCGATTGATTCCTCTATTCATTCGTGGAAATCATTTATTTTT
AGCCACTGATGATCCAAGCAAGCACGCGACAATAAAAGAAATTCAATTCTACACACAATTTTATCCTCTGGTGATCGTCG
TTGAAAGGGACAAATTAAATGGTTTAATTGAGAAAATTAACCAACATAAATCTAATCCTGGTTCTTACGAAATAGCAACG
ACTGAAAAATTTACTGAAAGATTGAGCGATCCGGGGTTTAAAGAGGATGCACCTATTGTTAAATTGGTACATGATATTCT
TGCGGACGCTGTTAAAAGGAAGGCATCGGATGTTCATTTTGAGCTTTATGAAGATCAATATCGGGTTCGCTATCGTTTAG
ATGGTGTTTTAGTCGAAGCTGTTGCACCGCCGACCAGTTTAGCAAATCAAATTACTTCTCGTATTAAAATTCTAGCTAAT
CTTGATATTTCCGAACGCCGTATACCTCAGGATGGACGATTTAGAATGGAGATGGCAGAAAGATTTACAATGGATTTTAG
AGTAAATGTCTGCCCTACTGTAAGTGGAGAAAAAATAGCTATCCGTATTCTTAATCCTAATATTTCTACGGTTAGTATTG
AAGAGCTCGGTTTTAATTCTTTTCAACAACAGTTATTTTTAAGATCCCTTGAGAAGCCTCAAGGTATGATTCTAATAACT
GGGCCTACGGGGAGTGGTAAAACAGCCTCTTTATATACAGCCCTAACTTTATTAAATACGCCTGAAAGAAATATTTTAAC
TGCCGAAGATCCGGTCGAAATAAAAATCTCTGGTATCAATCAAATAAATATTAATCCTAAAGTAGGCTTAACCTTTGCAA
ATGCACTTCGCTCTTTTTTACGTCAAGACCCAGATATCATTATGGTTGGGGAAATTCGAGATCTGGAAACAGCTGAAATT
GCAATTAAAGCATCTCATACAGGGCATCTTGTTCTCTCAACATTACACACTAATAGTGCAGCAGAAGCTTTAACACGCTT
AAGGAACATGGGAGTTCCCTCTTTTAACATTGCCAGTTCACTTAATCTTCTAATTGCTCAGCGTTTAGTTAGACGTTTAT
GTAGCTATTGCAAAAAAGTGACAAAGATTTTAACAGCTTCAGAACTTATTCAATTAGGATTTGAAAAAGAAATAATTAAC
CCTGTCGAGCTTTATAGCGCAGACGGTTGTAAACATTGTCTCAATGGTTACAAAGGCCGCATTGGCTTATTTGAAATACT
ACCGGTTTCTCCAACAATAGCCGATTTAATTCTCTCGAACGTTAATTCTATTGATATACTTCGACAAGCCAAACAGGAAG
GCATGTTGACTCTACGCCAGACAGGCTTTGAAAAAGTCAGAGATGGCATCACAACCATTGAAGAGTTAAATCGTGTTACC
ATTAACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(186-452)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

54.754

100

0.553
  pilB Acinetobacter baumannii D1279779

47.842

98.932

0.473
  pilB Acinetobacter baylyi ADP1

47.662

98.932

0.472
  pilB Vibrio cholerae strain A1552

44.602

100

0.448
  pilF Neisseria gonorrhoeae MS11

43.59

97.153

0.423
  pilB Vibrio campbellii strain DS40M4

41.519

100

0.418
  pilB Vibrio parahaemolyticus RIMD 2210633

42.674

97.153

0.415
  pilF Thermus thermophilus HB27

36.625

99.11

0.363