Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PY772_RS20900 Genome accession   NZ_CP118939
Coordinates   4445801..4447507 (+) Length   568 a.a.
NCBI ID   WP_223917248.1    Uniprot ID   A0AAV4YQ37
Organism   Aeromonas caviae strain K524     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
IScluster/Tn 4440753..4445365 4445801..4447507 flank 436


Gene organization within MGE regions


Location: 4440753..4447507
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PY772_RS20875 (PY772_20875) - 4440753..4441703 (-) 951 WP_005342491.1 IS1595-like element ISKpn3 family transposase -
  PY772_RS20880 (PY772_20880) - 4441760..4442920 (-) 1161 WP_275058888.1 ISL3-like element ISAeme19 family transposase -
  PY772_RS20885 (PY772_20885) - 4443329..4444279 (+) 951 WP_005342491.1 IS1595-like element ISKpn3 family transposase -
  PY772_RS20890 (PY772_20890) - 4444415..4445365 (+) 951 WP_103261601.1 IS1595-like element ISAeca5 family transposase -
  PY772_RS20895 (PY772_20895) - 4445381..4445797 (+) 417 WP_223917246.1 pilin -
  PY772_RS20900 (PY772_20900) pilB 4445801..4447507 (+) 1707 WP_223917248.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62602.94 Da        Isoelectric Point: 5.6747

>NTDB_id=796573 PY772_RS20900 WP_223917248.1 4445801..4447507(+) (pilB) [Aeromonas caviae strain K524]
MTSSPNSGLALSLAASSLISEEESQRYLSQAKAQRKPFVTFLIENDILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEESKLTAAIGKLMESEQDTLGIDHIDEAEI
SELEVSDESSRLDESVNTADDDAPIVKYIHKIMMDAIKRGASDLHFEPYETKYRIRFRVDGILHEVATPPVNLANRFAAR
LKVMARLDIAERRLPQDGRIKLKISRNRSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIDQLGFDDRQKTQYLHALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTSEVNISTAEDPVEINLPGVNQVQINPKAGLTFASALRSFLRQDPDIVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIIAQRLARKLCDHCKTPEVVPEAELLELGFT
QQQLAAGLRLFKPVGCKECSGGYRGRVGIYEIMLMSDNIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVTSLA
EINRITTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=796573 PY772_RS20900 WP_223917248.1 4445801..4447507(+) (pilB) [Aeromonas caviae strain K524]
ATGACTTCTAGCCCTAATAGCGGCCTGGCGTTAAGCCTGGCCGCCTCTTCACTGATAAGTGAAGAGGAGTCCCAGCGCTA
TCTAAGCCAGGCGAAGGCCCAGCGCAAACCCTTTGTCACCTTTCTGATAGAAAACGACATCCTCGACAGCAAGGCGCTCG
CCGATTTCTGCGAGCTGGAGTATGGGGTACCTCTGCTGGATCTGGCGGCCTTCGATCTCGCCGAGATCCCGCAGAAATAC
CTCAATCAAAAGCTGATTGAAAAGCACCATGTCCTGCCCATCTACACCCAGGGGCACACCCTCTATATCGCCATGTCGGA
CCCGACCAATGTGTCGGCTCTGGAGGATTTTGGTTTCAGTTTTGGCCTGCACACGGAAGCCCTGCTGGTCGAAGAGAGCA
AGCTGACCGCCGCCATCGGCAAGCTGATGGAGAGCGAGCAGGATACCCTGGGCATAGATCATATCGACGAGGCCGAGATC
TCCGAACTCGAGGTCTCCGACGAGAGTTCCCGCCTCGACGAGAGCGTCAACACCGCAGACGATGACGCCCCCATCGTCAA
ATACATCCACAAGATCATGATGGATGCCATCAAGCGCGGGGCCTCCGACCTGCATTTCGAACCCTACGAGACCAAGTACC
GGATCCGCTTTCGGGTAGACGGCATATTGCACGAAGTCGCCACGCCGCCGGTCAACCTGGCCAACCGCTTCGCGGCTCGC
CTCAAGGTGATGGCGCGGCTCGACATTGCCGAACGGCGCCTGCCCCAGGATGGCCGGATCAAGCTCAAGATCTCACGCAA
CAGATCCATGGACATGCGGGTCAACACCCTGCCCACCATGTGGGGCGAAAAGATCGTGATCCGGTTGCTGGACTCCTCGG
CCGCCCGGCTCAATATCGATCAACTCGGCTTTGATGACCGGCAGAAGACGCAATACCTGCACGCCCTCTCCAAACCTCAG
GGCATGATCCTGGTGACCGGCCCGACGGGGTCAGGCAAGACGGTCTCACTCTATACCGGCCTCAACATCCTCAACACCAG
CGAGGTCAATATCTCCACCGCCGAAGATCCGGTCGAGATAAACCTGCCCGGCGTCAACCAGGTGCAGATCAATCCCAAGG
CGGGGCTCACCTTCGCCAGCGCTCTGCGCTCCTTCCTGCGCCAGGATCCCGACATCGTCATGGTGGGGGAGATACGGGAT
CTGGAAACCGCCGAGATTGCTATCAAGGCCGCCCAGACCGGTCACCTGGTGCTCTCCACCCTGCACACCAACTCGGCGGC
AGAAACGCTCACCCGGATGATGAACATGGGGGTGCCCGCCTTCAATATCGCCTCCTCGGTGACCCTCATCATAGCCCAAC
GCCTAGCCCGCAAGCTGTGCGATCACTGCAAGACGCCGGAAGTGGTGCCGGAAGCCGAGTTGCTGGAGCTGGGCTTCACC
CAGCAGCAACTGGCCGCCGGGTTGCGGCTGTTCAAGCCCGTGGGATGCAAGGAGTGCTCGGGAGGCTACAGAGGCCGGGT
CGGCATCTATGAGATCATGCTGATGTCCGACAACATCGCCAAACTCATCATGCAGGGAGCCAACTCGCTGCAGATTGCCG
CGATAGCCCAAAAGGAAGGGATGCGCACCCTGCGCACATCCGGCCTCGAGAAAGCGCGTCTTGGCGTCACCAGCCTGGCC
GAAATCAACAGGATCACCACCAACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.203

99.824

0.551
  pilB Acinetobacter baylyi ADP1

54.912

100

0.551
  pilB Legionella pneumophila strain ERS1305867

56.458

95.423

0.539
  pilB Vibrio cholerae strain A1552

54.159

99.472

0.539
  pilB Vibrio campbellii strain DS40M4

51.805

97.535

0.505
  pilB Vibrio parahaemolyticus RIMD 2210633

50

98.944

0.495
  pilF Neisseria gonorrhoeae MS11

46.809

99.296

0.465
  pilF Thermus thermophilus HB27

40.152

92.958

0.373
  pilB/pilB1 Synechocystis sp. PCC 6803

38.267

97.535

0.373