Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   PY772_RS20345 Genome accession   NZ_CP118939
Coordinates   4327695..4328804 (+) Length   369 a.a.
NCBI ID   WP_275058877.1    Uniprot ID   -
Organism   Aeromonas caviae strain K524     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4322695..4333804
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PY772_RS20310 (PY772_20310) - 4322731..4323561 (+) 831 WP_042864458.1 CPBP family intramembrane glutamic endopeptidase -
  PY772_RS20315 (PY772_20315) - 4323637..4324056 (-) 420 WP_049636470.1 DUF4426 domain-containing protein -
  PY772_RS20320 (PY772_20320) yggU 4324075..4324374 (-) 300 WP_010673054.1 DUF167 family protein YggU -
  PY772_RS20325 (PY772_20325) - 4324374..4324925 (-) 552 WP_042014922.1 YggT family protein -
  PY772_RS20330 (PY772_20330) proC 4324949..4325773 (-) 825 WP_124814125.1 pyrroline-5-carboxylate reductase -
  PY772_RS20335 (PY772_20335) - 4325896..4326597 (-) 702 WP_042864460.1 YggS family pyridoxal phosphate-dependent enzyme -
  PY772_RS20340 (PY772_20340) pilT 4326637..4327671 (+) 1035 WP_010673050.1 type IVa pilus ATPase TapT Machinery gene
  PY772_RS20345 (PY772_20345) pilU 4327695..4328804 (+) 1110 WP_275058877.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PY772_RS20350 (PY772_20350) yaaA 4328837..4329610 (+) 774 WP_042882155.1 peroxide stress protein YaaA -
  PY772_RS20355 (PY772_20355) srmB 4329798..4331021 (-) 1224 WP_124814121.1 ATP-dependent RNA helicase SrmB -
  PY772_RS20360 (PY772_20360) - 4331149..4331859 (+) 711 WP_124814119.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  PY772_RS20365 (PY772_20365) brnQ 4332050..4333357 (+) 1308 WP_039040015.1 branched-chain amino acid transport system II carrier protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 40897.05 Da        Isoelectric Point: 6.3155

>NTDB_id=796570 PY772_RS20345 WP_275058877.1 4327695..4328804(+) (pilU) [Aeromonas caviae strain K524]
MNMDVLLAALVEQKGSDLFITVDAPPPLKVNGRLVSLGPAPLDKTTALALIRESLDETHFERFLHTREANYAIQRGSLGR
FRVSAFWQQDMPGMVLRRIETRIPTFDELVLPPILQEVAMAKRGLVLFVGATGAGKSTTQAAMIGFRNQHGDGHILTVED
PVEFVHQHDRCLVTQREVGIDTASFDVALKNSLRQAPDVILIGEIRSQETMEFAIQFAETGHLCLATLHANNANQALDRI
LHLVPQEKHRQFLFDLSFNLRAIVAQQLLPSAHGQRRVAAFEILLNTPLVTDIIRKGEMHRLKEVMTKSGELGMQTFDQA
LFTLFCAGQIGYSEALAHADSANDLRLLIKLSGREQLGAGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=796570 PY772_RS20345 WP_275058877.1 4327695..4328804(+) (pilU) [Aeromonas caviae strain K524]
ATGAACATGGATGTTCTGCTCGCCGCACTGGTTGAACAAAAGGGCTCGGATCTCTTCATCACGGTCGATGCTCCCCCGCC
CCTCAAGGTCAACGGGCGTCTGGTGTCGCTCGGACCAGCCCCCCTCGACAAGACGACAGCGCTGGCGCTGATCAGGGAGA
GCCTGGACGAGACCCACTTCGAGCGCTTCCTGCACACCCGCGAGGCCAACTACGCCATCCAGCGAGGCTCGCTGGGGCGC
TTTCGGGTCAGCGCCTTCTGGCAACAGGATATGCCGGGCATGGTGCTGCGCCGCATCGAGACCCGCATCCCCACCTTTGA
CGAGCTGGTGCTGCCTCCCATCCTGCAAGAGGTCGCCATGGCCAAACGCGGGCTGGTGCTCTTCGTCGGCGCCACCGGTG
CCGGCAAGTCCACCACCCAGGCGGCCATGATCGGCTTTCGCAACCAGCACGGTGACGGCCACATCCTGACGGTGGAGGAT
CCGGTGGAGTTCGTCCATCAGCACGATCGCTGCCTGGTGACCCAGCGGGAGGTGGGGATCGACACCGCATCCTTCGATGT
CGCCCTCAAAAATTCCCTGCGCCAGGCCCCGGATGTCATCCTCATCGGTGAAATTCGCTCCCAGGAGACCATGGAGTTCG
CCATCCAGTTCGCCGAGACGGGGCACCTCTGCCTCGCCACCTTGCACGCCAACAATGCCAACCAGGCGCTGGATCGCATC
CTGCATCTGGTCCCCCAGGAGAAGCACCGCCAGTTCCTGTTCGATCTCTCCTTCAACCTGCGCGCCATCGTCGCCCAGCA
GCTGCTCCCCTCCGCTCATGGGCAGCGCCGGGTCGCCGCCTTCGAGATCCTGCTCAACACCCCGCTCGTCACCGACATCA
TCCGCAAGGGAGAGATGCACAGACTCAAGGAGGTGATGACCAAATCCGGCGAGCTTGGCATGCAGACCTTCGATCAGGCG
CTGTTCACCCTGTTCTGCGCCGGCCAAATTGGCTACAGTGAGGCCCTTGCCCATGCCGACTCCGCCAACGACCTGCGACT
GCTCATCAAGCTGTCGGGTCGCGAGCAGCTGGGCGCAGGCACTCTCGACAACGTGACCCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.598

99.729

0.604
  pilU Pseudomonas stutzeri DSM 10701

58.974

95.122

0.561
  pilU Acinetobacter baylyi ADP1

57.79

95.664

0.553
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.857

91.057

0.39
  pilT Legionella pneumophila strain Lp02

40.816

92.954

0.379
  pilT Legionella pneumophila strain ERS1305867

40.816

92.954

0.379