Detailed information NTG0796500

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PX335_RS04810
• Genome accession: NZ_CP118933
• Coordinates: 988105..989223 (-)
• Length: 372 a.a.
• NCBI ID: WP_017386714.1
• Uniprot ID: A0A009I1Y6
• Organism: Acinetobacter pittii strain ML4
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 983105..994223

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PX335_RS04780 (PX335_04780)	-	983933..984781 (+)	849	WP_017386716.1	class II glutamine amidotransferase	-
PX335_RS04785 (PX335_04785)	-	985033..985488 (+)	456	WP_002121698.1	bacteriohemerythrin	-
PX335_RS04790 (PX335_04790)	-	985642..986709 (+)	1068	WP_029768342.1	membrane protein	-
PX335_RS04795 (PX335_04795)	-	986709..987023 (+)	315	WP_014205982.1	RnfH family protein	-
PX335_RS04800 (PX335_04800)	omlA	987088..987486 (-)	399	WP_002121740.1	outer membrane protein assembly factor BamE	-
PX335_RS04805 (PX335_04805)	fur	987598..988035 (+)	438	WP_002121758.1	ferric iron uptake transcriptional regulator	-
PX335_RS04810 (PX335_04810)	pilU	988105..989223 (-)	1119	WP_017386714.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
PX335_RS04815 (PX335_04815)	pilT	989243..990280 (-)	1038	WP_002121749.1	type IV pilus twitching motility protein PilT	Machinery gene
PX335_RS04820 (PX335_04820)	yggS	990407..991099 (+)	693	WP_017386713.1	YggS family pyridoxal phosphate-dependent enzyme	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41917.12 Da
• Isoelectric Point: 6.6404

>NTDB_id=796500 PX335_RS04810 WP_017386714.1 988105..989223(-) (pilU) [Acinetobacter pittii strain ML4]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEIIGQLLHSIMSEKQRKEFAETRECNFAIMNRDKSA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISYRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEVALKNTLRQAPDVILIGEIRTREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKSGQITYKDALKHADSPNDLRLTIKLAEEGPEQIAGTNQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=796500 PX335_RS04810 WP_017386714.1 988105..989223(-) (pilU) [Acinetobacter pittii strain ML4]
ATGGATTTTAATGACCTGCTCAACCTGATGGTTGAAAAAAAATCATCCGACCTCTTTATTACAGATGGCGTTGCACCATC
GATGAAGATTAACGGGCAAATTGTTCCCATTTCAAAAAATAGTCTTTCAGGCGAAATTATCGGGCAACTATTACACTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAGACTCGTGAATGTAACTTTGCGATTATGAACCGTGATAAAAGTGCG
CGCTTTCGTGTAAGTGCTTTTCAGCAACGCGACATGCCAGGCATGGTACTACGTCGAATTGAAACCAAAATTCCTTCAAT
TGACGACTTACAATTACCGCCAGTGCTTAAAGATTTATCGATGACCAAACGTGGCATCATTATTTTTGTAGGGGCAACAG
GTACAGGTAAATCGACATCATTGGCTTCAATGATTAGCTATCGTAACCATAACTCTAAAGGTCACATCATTACCATTGAA
GACCCTATCGAGTTTATTCATGAACATGCTGGCTGCATCATTACCCAACGTGAAGTCGGGATCGATACTGATTCATTTGA
AGTAGCTTTAAAGAATACTTTGCGACAAGCACCCGATGTAATCTTGATTGGTGAGATCCGTACGCGTGAAGTCATGGACT
ATGCGATTGGTTTTGCCGAAACGGGTCACTTGGTCTTAGCCACGATGCATGCGAACAACGCCAACCAAGCACTTGACCGT
ATTATTCACTTCTTTGAAAGTGACCGTCATAGCCAGCTCTACATGGATTTATCGCTTAATTTAAAGGCCATGATTGCACA
ACAACTTATTCCGACTCCAGATGGTAATTCGCGCCGTGCAGCGATTGAGATTCTCATTAACTCACCACTAATTTCAGACT
ATATTCGCAAAGGCGAAATTCATGAAATTAAAGATTTAATGAAACGCTCACGTGAACTCGGTATGCAAACTTTTGACCAA
GCACTGTTTGATCTCTATAAGTCAGGACAAATTACCTACAAAGATGCATTAAAACATGCCGACTCACCAAACGATTTACG
TTTAACAATTAAGCTTGCCGAAGAAGGTCCTGAGCAAATTGCAGGCACAAATCAGCATTTAACCTTCGACAGACAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (32-270)

3D structure

• AlphaFold DB: A0A009I1Y6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.132	100	0.763
pilU	Pseudomonas stutzeri DSM 10701	69.272	99.731	0.691
pilU	Vibrio cholerae strain A1552	54.054	99.462	0.538
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.934	97.849	0.401
pilT	Legionella pneumophila strain Lp02	39.941	90.86	0.363
pilT	Legionella pneumophila strain ERS1305867	39.941	90.86	0.363