Detailed information NTG0795060

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: PWO55_RS12415
• Genome accession: NZ_CP118770
• Coordinates: 2520504..2521118 (-)
• Length: 204 a.a.
• NCBI ID: WP_003152867.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain IMD4036
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2515504..2526118

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PWO55_RS12385 (PWO55_12375)	rpsT	2515804..2516070 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
PWO55_RS12390 (PWO55_12380)	holA	2516087..2517128 (-)	1042	Protein_2420	DNA polymerase III subunit delta	-
PWO55_RS12395 (PWO55_12385)	-	2517168..2517322 (-)	155	Protein_2421	hypothetical protein	-
PWO55_RS12400 (PWO55_12390)	-	2517363..2517497 (+)	135	WP_003152870.1	YqzM family protein	-
PWO55_RS12405 (PWO55_12395)	comEC	2517516..2519867 (-)	2352	WP_041481887.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
PWO55_RS12410 (PWO55_12400)	-	2519868..2520437 (-)	570	WP_003152868.1	ComE operon protein 2	-
PWO55_RS12415 (PWO55_12405)	comEA	2520504..2521118 (-)	615	WP_003152867.1	helix-hairpin-helix domain-containing protein	Machinery gene
PWO55_RS12420 (PWO55_12410)	comER	2521177..2521998 (+)	822	WP_014305449.1	late competence protein ComER	-
PWO55_RS12425 (PWO55_12415)	-	2522067..2522804 (-)	738	WP_032856885.1	class I SAM-dependent methyltransferase	-
PWO55_RS12430 (PWO55_12420)	rsfS	2522801..2523157 (-)	357	WP_003152864.1	ribosome silencing factor	-
PWO55_RS12435 (PWO55_12425)	yqeK	2523175..2523735 (-)	561	WP_032856887.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
PWO55_RS12440 (PWO55_12430)	-	2523725..2524294 (-)	570	WP_003152860.1	nicotinate-nucleotide adenylyltransferase	-
PWO55_RS12445 (PWO55_12435)	yhbY	2524305..2524595 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
PWO55_RS12450 (PWO55_12440)	aroE	2524589..2525431 (-)	843	WP_003152856.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22269.48 Da
• Isoelectric Point: 6.4940

>NTDB_id=795060 PWO55_RS12415 WP_003152867.1 2520504..2521118(-) (comEA) [Bacillus velezensis strain IMD4036]
MTKAMMTERMRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAVGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMKVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=795060 PWO55_RS12415 WP_003152867.1 2520504..2521118(-) (comEA) [Bacillus velezensis strain IMD4036]
ATGACGAAAGCGATGATGACGGAGCGGATGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCAGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGTCAAACAGCCTCCGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGAGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGTTGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAAGGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	59.375	94.118	0.559
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA	Latilactobacillus sakei subsp. sakei 23K	31.897	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363