Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   PWF74_RS10180 Genome accession   NZ_CP118627
Coordinates   2038116..2039135 (+) Length   339 a.a.
NCBI ID   WP_270252906.1    Uniprot ID   A0AAX3NEB9
Organism   Lactococcus garvieae strain Pa-2     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2033116..2044135
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PWF74_RS10160 (PWF74_10160) - 2033495..2035096 (-) 1602 WP_165705509.1 GH25 family lysozyme -
  PWF74_RS10165 (PWF74_10165) guaB 2035265..2036746 (+) 1482 WP_165705510.1 IMP dehydrogenase -
  PWF74_RS10170 (PWF74_10170) rpsU 2036869..2037045 (+) 177 WP_003134539.1 30S ribosomal protein S21 -
  PWF74_RS10175 (PWF74_10175) comGA 2037233..2038177 (+) 945 WP_165705597.1 competence type IV pilus ATPase ComGA Machinery gene
  PWF74_RS10180 (PWF74_10180) comGB 2038116..2039135 (+) 1020 WP_270252906.1 competence type IV pilus assembly protein ComGB Machinery gene
  PWF74_RS10185 (PWF74_10185) comYC 2039154..2039459 (+) 306 WP_416274244.1 competence type IV pilus major pilin ComGC Machinery gene
  PWF74_RS10190 (PWF74_10190) comGD 2039434..2039850 (+) 417 WP_165705512.1 competence type IV pilus minor pilin ComGD -
  PWF74_RS10195 (PWF74_10195) comGE 2039882..2040115 (+) 234 WP_229031266.1 competence type IV pilus minor pilin ComGE -
  PWF74_RS10200 (PWF74_10200) comGF 2040096..2040536 (+) 441 WP_165705513.1 competence type IV pilus minor pilin ComGF -
  PWF74_RS10205 (PWF74_10205) - 2040769..2041614 (+) 846 WP_165705514.1 metal ABC transporter solute-binding protein, Zn/Mn family -
  PWF74_RS10210 (PWF74_10210) - 2041700..2042569 (-) 870 WP_003133725.1 RluA family pseudouridine synthase -

Sequence

Protein


Download         Length: 339 a.a.        Molecular weight: 39550.25 Da        Isoelectric Point: 9.8215

>NTDB_id=794069 PWF74_RS10180 WP_270252906.1 2038116..2039135(+) (comGB) [Lactococcus garvieae strain Pa-2]
MDLSQLLQLRTKKLSLAKQIKLIQLMNNLFKSGFHLSEIIDFLERSGLTEAYFISKMREGLLNGQSFSGILAKLKFNEDI
VTQLSLAESHGNVEYTLGLIEEKLRRVLNIRKKLIQVATYPFVLLVFLIFIMLGLKNYLLPQLDENRGLATYVIQNLPTL
FLGFLFSLLIVFYLGRYYWKKKTALEAMRLMVKIPFVGRFVQLYLTAYFSREWGNLIAQAVDLRQICFIMQEQKSRIFKE
FGFELLHILDRGQKFEDALRFYPIFTKELALIVEYGELKSKLGKELMVFSEESWSLFFERVERAMQLIQPIVFLLVALLI
ILIYAAMLLPIYSNMGNLI

Nucleotide


Download         Length: 1020 bp        

>NTDB_id=794069 PWF74_RS10180 WP_270252906.1 2038116..2039135(+) (comGB) [Lactococcus garvieae strain Pa-2]
ATGGATTTATCTCAGCTGTTACAGCTGCGTACGAAAAAATTGAGTTTAGCGAAGCAGATTAAGCTTATTCAACTTATGAA
TAATCTTTTCAAAAGTGGCTTTCACCTTTCTGAAATTATTGATTTTCTTGAACGGTCGGGTCTCACGGAAGCATACTTTA
TTTCCAAAATGCGTGAAGGTTTACTCAACGGGCAAAGTTTCTCTGGTATTTTAGCGAAGCTCAAATTTAATGAGGATATT
GTGACTCAGCTCTCTTTGGCCGAGTCTCATGGGAATGTTGAGTATACTTTAGGGTTGATTGAAGAAAAACTGAGACGCGT
TTTGAACATTCGCAAAAAGCTCATCCAAGTTGCCACTTACCCGTTCGTTCTTCTGGTATTTCTAATCTTTATCATGCTAG
GGTTAAAAAATTATTTACTGCCTCAACTGGACGAAAACAGAGGATTAGCAACTTATGTTATACAGAATCTTCCGACTCTC
TTTTTAGGTTTCCTCTTCAGTTTGCTTATTGTCTTTTATCTGGGCAGATACTATTGGAAGAAAAAAACTGCTTTAGAAGC
GATGAGGCTAATGGTCAAAATACCTTTTGTTGGCCGTTTTGTCCAGCTTTATTTGACAGCTTATTTTTCAAGAGAATGGG
GAAACTTAATTGCTCAAGCAGTCGATTTACGTCAGATTTGTTTTATTATGCAAGAACAAAAAAGTCGAATCTTCAAAGAA
TTTGGTTTTGAGCTCCTTCATATATTAGATAGGGGTCAGAAGTTTGAAGACGCCCTTCGATTTTACCCCATATTTACCAA
GGAGTTGGCATTAATTGTGGAATATGGTGAACTAAAGTCTAAGCTAGGGAAAGAGTTAATGGTTTTTTCTGAAGAAAGCT
GGTCGCTCTTTTTTGAAAGAGTTGAAAGAGCAATGCAGCTTATCCAGCCTATCGTTTTCTTGCTCGTGGCGCTGTTGATT
ATTTTAATTTATGCTGCAATGTTACTTCCTATATATAGCAATATGGGAAACTTGATTTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(209-330)

Type II secretion system (T2SS), protein F (T2SSF)

(23-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Lactococcus lactis subsp. cremoris KW2

53.571

99.115

0.531
  comGB/cglB Streptococcus mitis NCTC 12261

52.836

98.82

0.522
  comYB Streptococcus gordonii str. Challis substr. CH1

52.381

99.115

0.519
  comGB/cglB Streptococcus mitis SK321

52.239

98.82

0.516
  comGB/cglB Streptococcus pneumoniae R6

51.94

98.82

0.513
  comGB/cglB Streptococcus pneumoniae Rx1

51.94

98.82

0.513
  comGB/cglB Streptococcus pneumoniae D39

51.94

98.82

0.513
  comGB/cglB Streptococcus pneumoniae TIGR4

51.94

98.82

0.513
  comYB Streptococcus mutans UA140

51.429

92.92

0.478
  comYB Streptococcus mutans UA159

51.111

92.92

0.475