Detailed information NTG0792801

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: PVT72_RS12010
• Genome accession: NZ_CP118495
• Coordinates: 2543975..2544589 (-)
• Length: 204 a.a.
• NCBI ID: WP_043867297.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain FC02
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2538975..2549589

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PVT72_RS11980 (PVT72_11980)	rpsT	2539275..2539541 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
PVT72_RS11985 (PVT72_11985)	holA	2539558..2540599 (-)	1042	Protein_2314	DNA polymerase III subunit delta	-
PVT72_RS11990 (PVT72_11990)	-	2540639..2540793 (-)	155	Protein_2315	hypothetical protein	-
PVT72_RS11995 (PVT72_11995)	-	2540834..2540968 (+)	135	WP_003152870.1	YqzM family protein	-
PVT72_RS12000 (PVT72_12000)	comEC	2540987..2543338 (-)	2352	WP_239819197.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
PVT72_RS12005 (PVT72_12005)	-	2543339..2543908 (-)	570	WP_003152868.1	ComE operon protein 2	-
PVT72_RS12010 (PVT72_12010)	comEA	2543975..2544589 (-)	615	WP_043867297.1	helix-hairpin-helix domain-containing protein	Machinery gene
PVT72_RS12015 (PVT72_12015)	comER	2544648..2545469 (+)	822	WP_115996552.1	late competence protein ComER	-
PVT72_RS12020 (PVT72_12020)	-	2545538..2546275 (-)	738	WP_014305450.1	class I SAM-dependent methyltransferase	-
PVT72_RS12025 (PVT72_12025)	rsfS	2546272..2546628 (-)	357	WP_239819198.1	ribosome silencing factor	-
PVT72_RS12030 (PVT72_12030)	yqeK	2546625..2547206 (-)	582	WP_003152863.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
PVT72_RS12035 (PVT72_12035)	-	2547196..2547765 (-)	570	WP_003152860.1	nicotinate-nucleotide adenylyltransferase	-
PVT72_RS12040 (PVT72_12040)	yhbY	2547776..2548066 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
PVT72_RS12045 (PVT72_12045)	aroE	2548060..2548902 (-)	843	WP_003152856.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22178.37 Da
• Isoelectric Point: 6.9685

>NTDB_id=792801 PVT72_RS12010 WP_043867297.1 2543975..2544589(-) (comEA) [Bacillus velezensis strain FC02]
MTKAMMTERMRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEGNHPKTAVGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMKVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=792801 PVT72_RS12010 WP_043867297.1 2543975..2544589(-) (comEA) [Bacillus velezensis strain FC02]
ATGACGAAAGCGATGATGACGGAGCGGATGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCAGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGTCAAACAGCCTCCGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGAGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGGGAATCATCCGAAAACAG
CAGTTGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAAGGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA	Latilactobacillus sakei subsp. sakei 23K	31.897	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363