Detailed information NTG0079208

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: HMSLTHF_RS01790
• Genome accession: NZ_AP022821
• Coordinates: 356945..358108 (+)
• Length: 387 a.a.
• NCBI ID: WP_083602229.1
• Uniprot ID: -
• Organism: Vreelandella aquamarina strain Slthf1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 351945..363108

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HMSLTHF_RS01765 (HMSLTHF_03450)	-	352266..353462 (-)	1197	WP_044629494.1	homoserine O-acetyltransferase	-
HMSLTHF_RS01770 (HMSLTHF_03460)	-	353536..354129 (-)	594	WP_172415842.1	YggT family protein	-
HMSLTHF_RS01775 (HMSLTHF_03470)	proC	354229..355056 (-)	828	WP_044629496.1	pyrroline-5-carboxylate reductase	-
HMSLTHF_RS01780 (HMSLTHF_03480)	-	355145..355843 (-)	699	WP_044629497.1	YggS family pyridoxal phosphate-dependent enzyme	-
HMSLTHF_RS01785 (HMSLTHF_03490)	pilT	355877..356920 (+)	1044	WP_074211079.1	type IV pilus twitching motility protein PilT	Machinery gene
HMSLTHF_RS01790 (HMSLTHF_03500)	pilU	356945..358108 (+)	1164	WP_083602229.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
HMSLTHF_RS01795 (HMSLTHF_03510)	-	358121..359263 (-)	1143	WP_074211639.1	anhydro-N-acetylmuramic acid kinase	-
HMSLTHF_RS01800 (HMSLTHF_03520)	tyrS	359380..360579 (+)	1200	WP_172415843.1	tyrosine--tRNA ligase	-

Sequence

Protein

• Length: 387 a.a.
• Molecular weight: 42135.21 Da
• Isoelectric Point: 6.8279

>NTDB_id=79208 HMSLTHF_RS01790 WP_083602229.1 356945..358108(+) (pilU) [Vreelandella aquamarina strain Slthf1]
MSEPASGMTVTQWLHQLLGIMVEQQASDLLISVGAPPSLKTPKGLMPLGRQPLSSSQVRSLVLNAVPEKQRERFQEEHEA
NFALSLESAGRFRVSAFQQRNEPAMVVRRIAQTIPTLSALGVPSQLATLANAKRGLVLVVGGTGTGKSTTLAAMIQERNA
SVGGHIITIEDPIEYLHPHQRGIVNQREVGVDTESFEVALKNTLRQAPDVILIGEVRTRETMEHALTFAETGHLCLATLH
ANNANQALERILHFFPHERHEQVLMDLSLNLRAVVAQQLLPTQQGGRCAAIEILLSSPRVVDLIRKGRIDELKQAMAGSR
DAGMQTFDQALYALYQAGQVSQQVALAHADSANDLRLMIKQGDAVASGKPLDAQVPPHLALRDGDDY

Nucleotide

• Length: 1164 bp

>NTDB_id=79208 HMSLTHF_RS01790 WP_083602229.1 356945..358108(+) (pilU) [Vreelandella aquamarina strain Slthf1]
ATGAGTGAGCCAGCCAGCGGTATGACCGTGACCCAGTGGCTGCACCAACTGCTGGGTATTATGGTCGAGCAGCAGGCATC
AGACCTGCTGATTTCGGTGGGCGCACCGCCCAGTTTGAAAACGCCCAAGGGGCTAATGCCGCTGGGGCGTCAGCCGCTCT
CCTCTAGCCAGGTGCGTAGCCTGGTTCTGAATGCGGTTCCCGAAAAGCAGCGGGAGCGATTTCAAGAGGAGCACGAGGCG
AACTTTGCCCTAAGCCTGGAGAGTGCGGGGCGGTTTCGGGTAAGCGCCTTTCAACAGCGCAACGAGCCCGCCATGGTGGT
GCGCCGCATTGCGCAGACCATTCCGACCCTTAGCGCGCTGGGCGTGCCTAGCCAGTTGGCGACGTTGGCCAATGCCAAGC
GCGGGCTGGTGCTAGTGGTAGGCGGCACGGGAACGGGGAAATCCACTACGCTGGCGGCGATGATTCAGGAGCGTAACGCC
TCAGTCGGTGGGCATATCATCACCATTGAAGACCCCATCGAGTACCTGCATCCGCATCAGCGAGGCATCGTTAATCAGCG
CGAGGTGGGGGTGGATACGGAGTCCTTCGAGGTGGCGCTGAAAAATACGCTACGCCAAGCGCCGGATGTGATCTTGATTG
GTGAAGTGCGTACCCGCGAGACCATGGAGCACGCCCTCACTTTCGCGGAAACCGGGCATCTCTGTTTGGCGACATTGCAC
GCCAACAACGCCAACCAAGCCTTAGAGCGTATCCTTCACTTCTTTCCCCATGAGCGGCACGAGCAGGTTCTGATGGATCT
GTCGCTGAATCTGCGTGCCGTGGTCGCGCAGCAGCTGCTACCTACTCAGCAGGGCGGCCGCTGCGCGGCGATCGAGATTC
TGCTCTCATCGCCGCGGGTGGTGGATCTGATTCGTAAAGGGCGTATTGATGAATTGAAGCAGGCCATGGCAGGCTCCCGC
GACGCGGGGATGCAAACCTTTGATCAGGCGCTTTATGCGCTTTATCAAGCGGGCCAAGTCAGCCAGCAAGTGGCGCTGGC
CCATGCGGACTCCGCCAACGACCTGCGGCTGATGATCAAGCAGGGCGACGCCGTCGCCAGCGGTAAGCCGTTAGATGCGC
AGGTGCCGCCCCATCTTGCTCTGCGCGACGGTGACGATTATTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (50-281)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	62.857	90.439	0.568
pilU	Vibrio cholerae strain A1552	58.939	92.506	0.545
pilU	Acinetobacter baylyi ADP1	55.679	93.282	0.519
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.091	96.382	0.406
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	42.988	84.755	0.364
pilT	Vibrio cholerae strain A1552	42.988	84.755	0.364