Detailed information NTG0791010

Bioinformatically predicted

Overview

• Name: rapF
• Type: Regulator
• Locus tag: PUW21_RS20335
• Genome accession: NZ_CP118167
• Coordinates: 3878819..3879964 (+)
• Length: 381 a.a.
• NCBI ID: WP_086344441.1
• Uniprot ID: -
• Organism: Bacillus subtilis strain 21855
• Function: inhibit the DNA-binding function of ComA (predicted from homology) Competence regulation

Genomic Context

Location: 3873819..3884964

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PUW21_RS20315 (PUW21_20315)	albF	3873835..3875115 (+)	1281	WP_003242974.1	pitrilysin family protein	-
PUW21_RS20320 (PUW21_20320)	albG	3875112..3875813 (+)	702	WP_003243391.1	antilisterial bacteriocin subtilosin biosynthesis protein AlbG	-
PUW21_RS20325 (PUW21_20325)	ywhL	3875819..3877195 (-)	1377	WP_110109508.1	YncE family protein	-
PUW21_RS20330 (PUW21_20330)	ywhK	3877234..3878589 (-)	1356	WP_015250986.1	YncE family protein	-
PUW21_RS22260	-	3878586..3878816 (-)	231	WP_024572000.1	hypothetical protein	-
PUW21_RS20335 (PUW21_20335)	rapF	3878819..3879964 (+)	1146	WP_086344441.1	response regulator aspartate phosphatase RapF	Regulator
PUW21_RS20340 (PUW21_20340)	phrF	3879948..3880067 (+)	120	WP_041334633.1	phosphatase RapF inhibitor PhrF	Regulator
PUW21_RS20345 (PUW21_20345)	-	3880165..3880627 (+)	463	Protein_3951	YbaK/EbsC family protein	-
PUW21_RS20350 (PUW21_20350)	speB	3880659..3881531 (-)	873	WP_003227545.1	agmatinase	-
PUW21_RS20355 (PUW21_20355)	speE	3881592..3882422 (-)	831	WP_003227543.1	spermidine synthase	-
PUW21_RS20360 (PUW21_20360)	pbpG	3882624..3884699 (+)	2076	WP_003227540.1	transglycosylase domain-containing protein	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 45631.11 Da
• Isoelectric Point: 5.1503

>NTDB_id=791010 PUW21_RS20335 WP_086344441.1 3878819..3879964(+) (rapF) [Bacillus subtilis strain 21855]
MTGVISSSSIGEKINEWYMYIRRFSIPDAEYLRREIKQELDQMEEDQDLHLYYSLMEFRHNLMLEYLEPLEKMRIEEQPR
LSDLLLEIDKKQARLTGLLEYYFNFFRGMYELDQREYLSAIKFFKKAESKLIFVKDRIEKAEFFFKMSESYYYMKQTYFS
MDYARQAYEIYKEHEAYNIRLLQCHSLFATNFLDLKQYEDAISHFQKAYSMAEAEKQPQLMGRTLYNIGLCKNSQSQYED
AITYFKRAIAVFEESNILPSLPQAYFLITQIHYKLGKMDKAHEYHSKGMAYSQKAGDVIYLSEFEFLKSLYLSGPDEEAI
QGFFDFLESKMLYADLEDFAIDVAKYYHERKNFQKASAYFLKVEQVRQLIQGGVSLYEIEV

Nucleotide

• Length: 1146 bp

>NTDB_id=791010 PUW21_RS20335 WP_086344441.1 3878819..3879964(+) (rapF) [Bacillus subtilis strain 21855]
GTGACAGGTGTCATATCTTCTTCTTCCATCGGAGAAAAGATTAACGAATGGTATATGTACATACGCCGATTCAGCATACC
CGATGCAGAATATTTGCGACGAGAAATCAAGCAAGAGCTGGATCAAATGGAAGAAGATCAAGACCTTCATTTGTACTATT
CACTGATGGAGTTTCGGCACAACCTGATGCTTGAGTACCTTGAACCGTTAGAAAAAATGAGGATTGAGGAACAGCCGAGA
CTGTCTGATCTGCTGCTTGAGATTGATAAAAAACAGGCTCGTTTAACTGGTCTGCTTGAGTACTATTTTAATTTCTTCAG
AGGCATGTATGAGCTGGACCAGCGGGAATATCTGTCGGCTATTAAATTTTTCAAAAAAGCCGAAAGCAAGCTGATATTCG
TTAAGGATCGGATAGAGAAAGCTGAGTTTTTCTTTAAGATGTCTGAATCTTATTACTATATGAAACAAACGTATTTTTCA
ATGGACTATGCACGGCAAGCATATGAAATATACAAAGAACATGAAGCTTATAATATAAGATTGCTGCAGTGTCATTCTTT
ATTTGCCACCAATTTTTTAGATTTAAAACAGTATGAGGATGCCATCTCACATTTTCAAAAAGCTTATTCTATGGCAGAAG
CTGAAAAGCAGCCCCAATTAATGGGGAGAACTTTGTACAATATCGGGCTTTGTAAAAACAGCCAAAGCCAATATGAGGAT
GCCATAACTTATTTCAAAAGAGCAATAGCTGTTTTTGAAGAATCAAATATTCTTCCTTCCTTACCTCAAGCGTATTTTTT
AATTACACAGATCCATTATAAATTAGGAAAAATGGATAAAGCTCATGAATATCATAGTAAGGGAATGGCTTATTCACAAA
AGGCCGGAGATGTAATATATTTATCAGAGTTTGAATTTTTGAAATCTTTATACTTATCAGGCCCGGATGAAGAAGCAATT
CAAGGATTTTTTGATTTTCTCGAAAGTAAAATGTTGTATGCTGATCTTGAAGATTTCGCTATTGATGTGGCAAAATATTA
TCATGAACGTAAAAATTTTCAAAAAGCTTCTGCTTATTTTTTGAAGGTGGAACAAGTAAGGCAACTTATTCAAGGAGGAG
TGAGTTTGTATGAAATTGAAGTCTAA

Domains

Predicted by InterproScan.

• Tetratricopeptide repeat (TPR_12) (223-290)

• response regulator aspartate phosphatase H, N terminal (RapH_N) (4-65)

• Anaphase-promoting complex, cyclosome, subunit 3 (ANAPC3) (132-208)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rapF	Bacillus subtilis subsp. subtilis str. 168	99.475	100	0.995
rapC	Bacillus subtilis subsp. subtilis str. 168	57.368	99.738	0.572